产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0499, RRID:AB_2833786.
展开/折叠
ADRBK1; Adrenergic beta receptor kinase 1; ARBK1_HUMAN; BARK; BARK1; Beta adrenergic receptor kinase 1; Beta ARK 1; Beta ARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; FLJ16718; G protein coupled receptor kinase 2; G-protein coupled receptor kinase 2; GRK2;
抗原和靶标
Purified recombinant fragment of human GRK2 expressed in E. Coli.
- P25098 ARBK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL
翻译修饰 - P25098 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y13 | Phosphorylation | P00533 (EGFR) , P12931 (SRC) | Uniprot |
| S29 | Phosphorylation | P17252 (PRKCA) , P05129 (PRKCG) , Q05655 (PRKCD) | Uniprot |
| K31 | Ubiquitination | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| R50 | Methylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| Y86 | Phosphorylation | P00533 (EGFR) , P12931 (SRC) | Uniprot |
| Y92 | Phosphorylation | P12931 (SRC) , P00533 (EGFR) | Uniprot |
| K94 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| Y206 | Phosphorylation | Uniprot | |
| T213 | Phosphorylation | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| Y356 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| K494 | Ubiquitination | Uniprot | |
| K497 | Ubiquitination | Uniprot | |
| S501 | Phosphorylation | Uniprot | |
| Y506 | Phosphorylation | Uniprot | |
| K545 | Ubiquitination | Uniprot | |
| K557 | Ubiquitination | Uniprot | |
| K628 | Ubiquitination | Uniprot | |
| K644 | Acetylation | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| S670 | Phosphorylation | P27361 (MAPK3) , P24941 (CDK2) | Uniprot |
| S676 | Phosphorylation | Uniprot | |
| K677 | Ubiquitination | Uniprot | |
| R683 | Methylation | Uniprot | |
| S685 | Phosphorylation | P17612 (PRKACA) | Uniprot |
翻译修饰 - P25098 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P05387 (RPLP2) | S102 | Uniprot |
| P05387 (RPLP2) | S105 | Uniprot |
| P07550 (ADRB2) | S355 | Uniprot |
| P07550 (ADRB2) | S356 | Uniprot |
| P07550 (ADRB2) | T384 | Uniprot |
| P07550 (ADRB2) | S396 | Uniprot |
| P07550 (ADRB2) | S401 | Uniprot |
| P07550 (ADRB2) | S407 | Uniprot |
| P07550 (ADRB2) | S411 | Uniprot |
| P08069 (IGF1R) | S1278 | Uniprot |
| P08172 (CHRM2) | S232 | Uniprot |
| P08172 (CHRM2) | S234 | Uniprot |
| P08172 (CHRM2) | S250 | Uniprot |
| P08172 (CHRM2) | S251 | Uniprot |
| P08172 (CHRM2) | T271 | Uniprot |
| P08172 (CHRM2) | S282 | Uniprot |
| P08172 (CHRM2) | S283 | Uniprot |
| P08172 (CHRM2) | S286 | Uniprot |
| P08172 (CHRM2) | T287 | Uniprot |
| P08172 (CHRM2) | S288 | Uniprot |
| P08172 (CHRM2) | S290 | Uniprot |
| P08172 (CHRM2) | S294 | Uniprot |
| P08172 (CHRM2) | T302 | Uniprot |
| P08172 (CHRM2) | T307 | Uniprot |
| P08172 (CHRM2) | S309 | Uniprot |
| P08172 (CHRM2) | T310 | Uniprot |
| P08172 (CHRM2) | S311 | Uniprot |
| P09497 (CLTB) | S205 | Uniprot |
| P15311 (EZR) | T567 | Uniprot |
| P18545 (PDE6G) | T62 | Uniprot |
| P21731-2 (TBXA2R) | S239 | Uniprot |
| P21731-2 (TBXA2R) | S357 | Uniprot |
| P26038 (MSN) | T558 | Uniprot |
| P30411 (BDKRB2) | S366 | Uniprot |
| P30411 (BDKRB2) | S373 | Uniprot |
| P30411 (BDKRB2) | S375 | Uniprot |
| P32245 (MC4R) | T312 | Uniprot |
| P32245 (MC4R) | S329 | Uniprot |
| P32245 (MC4R) | S330 | Uniprot |
| P35241 (RDX) | T564 | Uniprot |
| P35367 (HRH1) | S398 | Uniprot |
| P35372 (OPRM1) | T356 | Uniprot |
| P35372 (OPRM1) | S357 | Uniprot |
| P35372 (OPRM1) | S358 | Uniprot |
| P35372 (OPRM1) | S377 | Uniprot |
| P37840 (SNCA) | S129 | Uniprot |
| P41143 (OPRD1) | T358 | Uniprot |
| P41143 (OPRD1) | S363 | Uniprot |
| Q14940 (SLC9A5) | S702 | Uniprot |
| Q14940 (SLC9A5) | S709 | Uniprot |
| Q14940 (SLC9A5) | S711 | Uniprot |
| Q14940 (SLC9A5) | S712 | Uniprot |
| Q14940 (SLC9A5) | T714 | Uniprot |
| Q15796 (SMAD2) | T197 | Uniprot |
| Q16539 (MAPK14) | T123 | Uniprot |
| Q96A54 (ADIPOR1) | S7 | Uniprot |
| Q96A54 (ADIPOR1) | T24 | Uniprot |
| Q96A54 (ADIPOR1) | T53 | Uniprot |
| Q9Y2W7 (KCNIP3) | S95 | Uniprot |
研究背景
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity).
Cytoplasm. Cell membrane.
Expressed in peripheral blood leukocytes.
Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1. Interacts with LPAR1 and LPAR2. Interacts with RALA in response to LPAR1 activation. ADRBK1 and RALA mutually inhibit each other's binding to LPAR1. Interacts with ADRB2.
The PH domain binds anionic phospholipids and helps recruiting ADRBK1 from the cytoplasm to plasma membrane close to activated receptors. It mediates binding to G protein beta and gamma subunits, competing with G-alpha subunits and other G-betagamma effectors.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Morphine addiction.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Sensory system > Olfactory transduction.
限制条款
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