产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0568, RRID:AB_2833814.
展开/折叠
GATA 3; GATA binding factor 3; GATA binding protein 3; GATA-binding factor 3; Gata3; GATA3_HUMAN; HDR; HDRS; MGC2346; MGC5199; MGC5445; Trans acting T cell specific transcription factor GATA 3; Trans-acting T-cell-specific transcription factor GATA-3;
抗原和靶标
Purified recombinant fragment of human GATA3 expressed in E. Coli.
- P23771 GATA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSHSRGSMTALGGASSSTHHPITTYPPYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTGRECVNCGATSTPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTSCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNINRPLTMKKEGIQTRNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSSHMLTTPTPMHPPSSLSFGPHHPSSMVTAMG
翻译修饰 - P23771 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| S110 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| T156 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S162 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| Y186 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| R261 | Methylation | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| Y282 | Phosphorylation | Uniprot | |
| Y290 | Phosphorylation | Uniprot | |
| K292 | Acetylation | Uniprot | |
| K304 | Acetylation | Uniprot | |
| S308 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T315 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| Y344 | Phosphorylation | Uniprot | |
| K346 | Acetylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| K371 | Acetylation | Uniprot | |
| K373 | Acetylation | Uniprot | |
| K374 | Acetylation | Uniprot | |
| K376 | Acetylation | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| S381 | Phosphorylation | Uniprot |
研究背景
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
Nucleus.
T-cells and endothelial cells.
Interacts with TBX21 ('Tyr-530' phosphorylated form).
Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation.
The YxKxHxxxRP motif is critical for DNA-binding and function.
研究领域
· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
限制条款
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