产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0440, RRID:AB_2833844.
展开/折叠
Endothelial Kruppel like zinc finger protein; Epithelial zinc finger protein EZF; EZF; GKLF; gut Kruppel-like factor; Gut-enriched krueppel-like factor; KLF; KLF4; KLF4_HUMAN; Krueppel-like factor 4; Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor); Kruppel like factor 4 (gut);
抗原和靶标
Purified recombinant fragment of human KLF4 expressed in E. Coli.
- O43474 KLF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQGQSRGFVARAGEPCVCWPHFGTHGMMLTPPSSPLELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHF
翻译修饰 - O43474 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K32 | Ubiquitination | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| K278 | Sumoylation | Uniprot | |
| T316 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| K448 | Sumoylation | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot |
研究背景
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Nucleus.
Interacts with POU5F1/OCT4 and SOX2 (By similarity). Interacts with MUC1 (via the C-terminal domain). Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a. Interacts with ZNF296 (By similarity). Interacts with GLIS1.
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Belongs to the krueppel C2H2-type zinc-finger protein family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
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