产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0062, RRID:AB_2833855.
展开/折叠
CCNB 1; CCNB; ccnb1; CCNB1_HUMAN; Cyclin B1; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1;
抗原和靶标
Purified recombinant fragment of human CCNB1 expressed in E. Coli.
- P14635 CCNB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAYLRQLEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVPKKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRPLPLHFLRRASKIGEVDVEQHTLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV
翻译修饰 - P14635 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T6 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| K25 | Acetylation | Uniprot | |
| K25 | Ubiquitination | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| K73 | Acetylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| K111 | Sumoylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | P06493 (CDK1) , P28482 (MAPK1) , P53350 (PLK1) | Uniprot |
| S128 | Phosphorylation | P06493 (CDK1) , P53350 (PLK1) , P28482 (MAPK1) | Uniprot |
| S133 | Phosphorylation | P53350 (PLK1) , Q9H4B4 (PLK3) | Uniprot |
| S147 | Phosphorylation | P53350 (PLK1) | Uniprot |
| Y177 | Phosphorylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| T362 | Phosphorylation | Uniprot | |
| T395 | Phosphorylation | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| K428 | Ubiquitination | Uniprot |
翻译修饰 - P14635 作为激酶
研究背景
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3. Interacts with RFPL4A and UBE2A (By similarity). Interacts with INCA1.
Belongs to the cyclin family. Cyclin AB subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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