SLC1A4 Antibody - #DF7178

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产品: SLC1A4 抗体
货号: DF7178
描述: Rabbit polyclonal antibody to SLC1A4
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 56kDa; 56kD(Calculated).
蛋白号: P43007
RRID: AB_2839130

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
克隆:
Polyclonal
特异性:
SLC1A4 Antibody detects endogenous levels of total SLC1A4.
RRID:
AB_2839130
引用格式: Affinity Biosciences Cat# DF7178, RRID:AB_2839130.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alanine/serine/cysteine/threonine transporter 1; ASCT-1; ASCT1; AW045657; Glutamate/neutral amino acid transporter; Neutral amino acid transporter A; OTTHUMP00000159933; OTTHUMP00000235138; SATT; SATT_HUMAN; SLC1A4; Solute carrier family 1 (glutamate/neutral amino acid transporter), member 4; Solute carrier family 1 member 4;

抗原和靶标

免疫原:
Uniprot:

P43007(SATT_HUMAN) >>Visit HPA database.

基因/基因ID:
SLC1A4(6509)
表达:
P43007 SATT_HUMAN:

Expressed mostly in brain, muscle, and pancreas but detected in all tissues examined.

描述:
SLC1A4, also known as ASCT1, is a neutral amino acid transporter. Its other name, ASCT1, was given because it mediates obligatory exchange of alanine, serine, cysteine, and threonine (1). SLC1A4 mediates the efflux of glutamate from the neuron into the synaptic junction via calcium-independent release, as well as mediating the efflux of L-serine from glial cells and its uptake by neurons (2). SLC1A4-mediated transport is shown to involve a symmetrical potassium-independent electroneutral exchange of neutral amino acids and sodium, such that the current activated during transport is carried only by chloride ions (3).
序列:
MEKSNETNGYLDSAQAGPAAGPGAPGTAAGRARRCAGFLRRQALVLLTVSGVLAGAGLGAALRGLSLSRTQVTYLAFPGEMLLRMLRMIILPLVVCSLVSGAASLDASCLGRLGGIAVAYFGLTTLSASALAVALAFIIKPGSGAQTLQSSDLGLEDSGPPPVPKETVDSFLDLARNLFPSNLVVAAFRTYATDYKVVTQNSSSGNVTHEKIPIGTEIEGMNILGLVLFALVLGVALKKLGSEGEDLIRFFNSLNEATMVLVSWIMWYVPVGIMFLVGSKIVEMKDIIVLVTSLGKYIFASILGHVIHGGIVLPLIYFVFTRKNPFRFLLGLLAPFATAFATCSSSATLPSMMKCIEENNGVDKRISRFILPIGATVNMDGAAIFQCVAAVFIAQLNNVELNAGQIFTILVTATASSVGAAGVPAGGVLTIAIILEAIGLPTHDLPLILAVDWIVDRTTTVVNVEGDALGAGILHHLNQKATKKGEQELAEVKVEAIPNCKSEEETSPLVTHQNPAGPVASAPELESKESVL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
92
Chicken
92
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P43007 作为底物

Site PTM Type Enzyme
M1 Acetylation
T7 Phosphorylation
Y10 Phosphorylation
K196 Ubiquitination
N201 N-Glycosylation
N206 N-Glycosylation
S242 Phosphorylation
K483 Ubiquitination
K484 Ubiquitination
K493 Ubiquitination
K501 Ubiquitination
S502 Phosphorylation
T506 Phosphorylation
S507 Phosphorylation
S521 Phosphorylation
S527 Phosphorylation
K528 Ubiquitination
S530 Phosphorylation

研究背景

功能:

Transporter for alanine, serine, cysteine, and threonine. Exhibits sodium dependence.

细胞定位:

Membrane>Multi-pass membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed mostly in brain, muscle, and pancreas but detected in all tissues examined.

蛋白家族:

Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A4 subfamily.

文献引用

1). Metformin attenuates angiotensin II‑induced cardiomyocyte hypertrophy by upregulating the MuRF1 and MAFbx pathway. Experimental and Therapeutic Medicine, 2021 (PubMed: 34539827) [IF=2.4]

Application: WB    Species: Rat    Sample: H9c2 cells

Figure 3 Effects of metformin on MuRF1 and MAFbx expression. (A) Western blot analysis measuring the expression of MuRF1 and MAFbx protein after the indicated treatments. (B) Densitometric analysis of MuRF1 and MAFbx expression in cardiomyocytes. n=3 for each group. *P<0.05. #P<0.05. MuRF1, muscle RING-finger protein-1; MAFbx, muscle atrophy F-box; Ang, angiotensin; Met, metformin.

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