产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7228, RRID:AB_2839169.
展开/折叠
Dual specificity phosphatase 6; Dual specificity phosphatase 6 isoform a; Dual specificity protein phosphatase 6; Dual specificity protein phosphatase PYST1; DUS6_HUMAN; DUSP 6; DUSP 6a; Dusp6; DUSP6a; HH19; MAP kinase phosphatase 3; Mitogen activated protein kinase phosphatase 3; Mitogen-activated protein kinase phosphatase 3; MKP 3; MKP-3; MKP3; PYST 1; PYST1; Serine/threonine specific protein phosphatase;
抗原和靶标
- Q16828 DUS6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIDTLRPVPFASEMAISKTVAWLNEQLELGNERLLLMDCRPQELYESSHIESAINVAIPGIMLRRLQKGNLPVRALFTRGEDRDRFTRRCGTDTVVLYDESSSDWNENTGGESVLGLLLKKLKDEGCRAFYLEGGFSKFQAEFSLHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDIESDLDRDPNSATDSDGSPLSNSQPSFPVEILPFLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFIDEARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQQLYFTTPSNQNVYQVDSLQST
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q16828 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K68 | Ubiquitination | Uniprot | |
| S159 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S174 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S178 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| K254 | Ubiquitination | Uniprot | |
| Y255 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| K324 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot |
研究背景
Inactivates MAP kinases. Has a specificity for the ERK family. Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (By similarity). Promotes cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors.
Cytoplasm.
Expressed in keratinocytes (at protein level).
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)
限制条款
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