His-Tag Antibody - #T0009
(15)
(4)
产品描述
来源:
Mouse
应用:
WB 1:3000-1:10000, IF/ICC: 1:200, IP 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
All
克隆:
Monoclonal [T113]
特异性:
His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal 6His -tagged proteins.
RRID:
AB_2839416
引用格式: Affinity Biosciences Cat# T0009, RRID:AB_2839416.
引用格式: Affinity Biosciences Cat# T0009, RRID:AB_2839416.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
his tag
抗原和靶标
免疫原:
A synthetic peptide HHHHHH coupled to KLH.
描述:
N/A
文献引用
1). Discovery of a potent allosteric activator of DGKQ that ameliorates obesity-induced insulin resistance via the sn-1,2-DAG-PKCε signaling axis. Cell metabolism
(PubMed: 36525963)
[IF=29.0]
2). Identification of human LDHC4 as a potential target for anticancer drug discovery. Acta Pharmaceutica Sinica B
(PubMed: 35646544)
[IF=14.5]
Application: WB Species: Mice Sample: A549 cells
Figure 2
Overexpression of LDHC promotes tumor growth in mice. (A) The nude mice inoculated with A549 cells overexpressing LDHC (upper) and with control A549 cells (lower), the picture was taken on the 45th day after inoculation. (B) Tumor growth curves of the LDHC-overexpression group (red) and the control group (black). The volumes of the tumors overexpressing LDHC are significantly higher than that of the control tumors (n = 5, data are mean ± SD, ∗∗∗P < 0.001). (C) The picture of the tumors from the LDHC-overexpression group and the control group with a ruler to show the diameters of the tumors. (D) Statistical analysis of tumor mass. The masses of the tumors overexpressing LDHC are significantly higher than that of the control tumors (n = 5, data are mean ± SD, ∗∗∗P < 0.001). (E) Detection of His6-tag of the recombinant LDHC in tumors by Western blot. Mice 1 to 5 were inoculated with the LDHC-overexpression A549 cells, while mice 6 to 10 were inoculated with the control A549 cells. HEK 293 cells were used as the positive control.
3). pH-responsive nanocomposite hydrogel for simultaneous prevention of postoperative adhesion and tumor recurrence. Acta Biomaterialia
(PubMed: 36563777)
[IF=9.7]
4). Multifunctional human serum albumin fusion protein as a docetaxel nanocarrier for chemo-photothermal synergetic therapy of ovarian cancer. ACS Applied Materials & Interfaces
(PubMed: 35441508)
[IF=9.5]
5). Staphylococcal superantigen-like protein 10 induces necroptosis through TNFR1 activation of RIPK3-dependent signal pathways. Communications Biology
(PubMed: 35962126)
[IF=5.9]
6). The 1316T> C missenses mutation in MTHFR contributes to MTHFR deficiency by targeting MTHFR to proteasome degradation. Aging-US
(PubMed: 33290257)
[IF=5.2]
7). Recombinant expression a novel fibronectin—collage fusion peptide modulating stem cell stemness via integrin β3. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(PubMed: 35590080)
[IF=5.0]
8). Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication. Veterinary Research
(PubMed: 32962745)
[IF=4.4]
Application: WB Species: Sample:
Figure 1| Identifcation of the interaction between the NDV M protein and chBRD2 protein.C A Coomassie stained gel showing the bacterial expression purifed proteins. The His-tagged chBRD2 (His-chBRD2), His tag,GST-tagged M (GST-M), and GST tag were expressed in E. coli BL21 (DE3), and the soluble His-chBRD2 and His tag or GST-M and GST tag were purifed on Ni–NTA His*Bind Resin or Glutathione-Sepharose 4B beads, respectively. The bacterial expression purifed proteins were detected by SDS-PAGE along with Coomassie blue staining. Identifcation of the interaction between the M protein and chBRD2 protein by His pull-down assay (D) and GST pull-down assay (E).
9). Molecular and Structural Basis of Receptor Binding and Signaling of a Fish Type I IFN with Three Disulfide Bonds. The Journal of Immunology
(PubMed: 35906001)
[IF=4.4]
10). Canine Parvovirus is diagnosed and neutralized by chicken IgY-scFv generated against the virus capsid protein. VETERINARY RESEARCH
(PubMed: 32883344)
[IF=4.4]
Application: WB Species: Sample: CRFK cells
Figure 7 |Neutralization efect of obtained scFv to CPV. A scFv gene was ligated pcMV-3 vector. B Immunofuorescence analysis of scFv transfected CRFK cells. control: CRFK cells of pcMV-3 without scFv, scFv: CRFK cells of pcMV-3 with scFv, DAPI: staining nuclei. C western blot detection of scFv transfected CRFK cells, control represent empty pcMV-3 transfected CRFK cells.
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