Tubulin beta Antibody | Affinity Biosciences LTD|亲科生物官网

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FIGURE 2 Enhanced green fluorescent protein in the vmhcKI–EGFP zebrafish KI line recapitulates endogenous expression patterns of the vmhc gene.

产品:	Tubulin beta 抗体
货号:	T0034
描述:	Mouse monoclonal antibody to Tubulin beta
应用:	WB ELISA
反应:	Zebrafish
分子量:	55 KD; 50kD(Calculated).
蛋白号:	P07437
RRID:	AB_2839425

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规格	价格	库存
50ul	RMB¥ 450	现货
100ul	RMB¥ 750	现货
200ul	RMB¥ 1000	现货

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实验方案

产品描述

来源:

Mouse

应用:

WB 1:3000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Zebrafish

克隆:

Monoclonal [F301]

特异性:

Theβ-tubulin antibody can detects Zebrafish endogenousβ-tubulin protein.

RRID:

AB_2839425
引用格式: Affinity Biosciences Cat# T0034, RRID:AB_2839425.

偶联:

Unconjugated.

纯化:

affinity purification.

保存:

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C. Do not aliquot the antibody. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

TUBB, Beta Tubulin, Beta 5-tubulin, Beta-4 tubulin, Beta1-tubulin, M40, Tubulin beta-5 chain, Beta Ib tubulin, Class I beta-tubulin, OK/SW-cl.56, TUBB5, Tubulin beta chain, Tubulin beta polypeptide, Tubulin beta-1 chain, Tubulin, beta class I, Tubuli ...

抗原和靶标

免疫原:

Peptide.

Uniprot:

P07437(TBB5_HUMAN) >>Visit HPA database.

基因/基因ID:

TUBB(203068)

表达:

P07437 TBB5_HUMAN:

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

描述:

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

序列:

P07437 TBB5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA

翻译修饰 - P07437 作为底物

P07437

Site	PTM Type	Enzyme	Source
R2	Methylation		Uniprot
C12	S-Nitrosylation		Uniprot
K19	Acetylation		Uniprot
K19	Methylation		Uniprot
K19	Ubiquitination		Uniprot
S25	Phosphorylation		Uniprot
T33	Phosphorylation		Uniprot
T35	Phosphorylation		Uniprot
Y36	Phosphorylation		Uniprot
S40	Phosphorylation		Uniprot
R46	Methylation		Uniprot
S48	Phosphorylation		Uniprot
Y50	Phosphorylation		Uniprot
Y51	Phosphorylation		Uniprot
T55	Phosphorylation		Uniprot
K58	Acetylation		Uniprot
K58	Methylation		Uniprot
K58	Sumoylation		Uniprot
K58	Ubiquitination		Uniprot
Y59	Phosphorylation		Uniprot
T72	Phosphorylation		Uniprot
S75	Phosphorylation		Uniprot
S78	Phosphorylation		Uniprot
S95	Phosphorylation		Uniprot
K103	Acetylation		Uniprot
K103	Sumoylation		Uniprot
K103	Ubiquitination		Uniprot
Y106	Phosphorylation		Uniprot
T107	Phosphorylation		Uniprot
S115	Phosphorylation		Uniprot
K122	Ubiquitination		Uniprot
S126	Phosphorylation		Uniprot
T136	Phosphorylation		Uniprot
S138	Phosphorylation		Uniprot
T143	Phosphorylation		Uniprot
S145	Phosphorylation		Uniprot
T149	Phosphorylation		Uniprot
S153	Phosphorylation		Uniprot
K154	Ubiquitination		Uniprot
Y159	Phosphorylation		Uniprot
R162	Methylation		Uniprot
T166	Phosphorylation		Uniprot
S168	Phosphorylation		Uniprot
S172	Phosphorylation		Uniprot
K174	Ubiquitination		Uniprot
Y183	Phosphorylation		Uniprot
T196	Phosphorylation		Uniprot
T199	Phosphorylation		Uniprot
Y200	Phosphorylation		Uniprot
Y208	Phosphorylation		Uniprot
K216	Ubiquitination		Uniprot
T218	Phosphorylation		Uniprot
T219	Phosphorylation		Uniprot
T221	Phosphorylation		Uniprot
Y222	Phosphorylation		Uniprot
S234	Phosphorylation		Uniprot
C239	S-Nitrosylation		Uniprot
K252	Sumoylation		Uniprot
K252	Ubiquitination		Uniprot
T274	Phosphorylation		Uniprot
S275	Phosphorylation	Q5TCY1 (TTBK1)	Uniprot
R276	Methylation		Uniprot
S278	Phosphorylation		Uniprot
Y281	Phosphorylation		Uniprot
T285	Phosphorylation		Uniprot
T290	Phosphorylation		Uniprot
K297	Ubiquitination		Uniprot
C303	S-Nitrosylation		Uniprot
Y310	Phosphorylation		Uniprot
T312	Phosphorylation		Uniprot
R318	Methylation		Uniprot
S322	Phosphorylation		Uniprot
K324	Acetylation		Uniprot
K324	Sumoylation		Uniprot
K324	Ubiquitination		Uniprot
K336	Acetylation		Uniprot
K336	Ubiquitination		Uniprot
S338	Phosphorylation		Uniprot
S339	Phosphorylation		Uniprot
Y340	Phosphorylation		Uniprot
K350	Sumoylation		Uniprot
K350	Ubiquitination		Uniprot
T351	Phosphorylation		Uniprot
K362	Ubiquitination		Uniprot
T366	Phosphorylation		Uniprot
S371	Phosphorylation		Uniprot
K379	Acetylation		Uniprot
K379	Ubiquitination		Uniprot
S382	Phosphorylation		Uniprot
T386	Phosphorylation		Uniprot
K392	Ubiquitination		Uniprot
T399	Phosphorylation		Uniprot
Y422	Phosphorylation		Uniprot
Y425	Phosphorylation		Uniprot
T429	Phosphorylation		Uniprot

研究背景

P07437_TBB5_HUMAN

功能:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

翻译修饰:

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.

Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

细胞定位:

Cytoplasm>Cytoskeleton.

组织特异性:

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

亚基结构:

Heterodimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with PIFO. Interacts with DIAPH1. Interacts with MX1 (By similarity). May interact with RNABP10 (By similarity). Interacts with CFAP157 (By similarity).

蛋白家族:

The highly acidic C-terminal region may bind cations such as calcium.

Belongs to the tubulin family.

研究领域

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

文献引用

1). An EGFP Knock-in Zebrafish Experimental Model Used in Evaluation of the Amantadine Drug Safety During Early Cardiogenesis. Frontiers in Cardiovascular Medicine, 2022 (PubMed: 35449877) [IF=3.6]

Application: WB Species: Fish Sample: vmhcKI–EGFP KI fish line

FIGURE 2 Enhanced green fluorescent protein in the vmhcKI–EGFP zebrafish KI line recapitulates endogenous expression patterns of the vmhc gene. (A) EGFP patterns in the vmhcKI–EGFP KI fish line from 16 to 18 somite stage (SS) to 48 hpf. Scale bar, 100 μm. White arrows point to specific patterns of the EGFP signal. (B) Endogenous expression patterns of the vmhc gene from 16 to 18 somite stage (SS) to 48 hpf revealed by WISH. Black arrows point to the specific expression pattern of vmhc endogenous transcripts. The dotted line outlines the shape of the atrium. Scale bar, 100 μm. (C) Western blotting and quantification analysis of the Vmhc protein expression in the vmhcKI–EGFP KI fish line compared to wild-type (WT) control. N = 4. Unpaired 2-tailed Student’s t-test. V, ventricle. A, atrium.

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Tubulin beta Antibody - #T0034

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