产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0563, RRID:AB_2833890.
展开/折叠
AIS; Amplified in squamous cell carcinoma; B(p51A); B(p51B); Chronic ulcerative stomatitis protein; CUSP; DN p63 alpha 1; DNp63; EEC3; id:ibd3516; Keratinocyte transcription factor; Keratinocyte transcription factor KET; KET; LMS; MGC115972; MGC192897; NBP; OFC8; OTTHUMP00000209732; OTTHUMP00000209733; OTTHUMP00000209734; OTTHUMP00000209735; OTTHUMP00000209737; OTTHUMP00000209738; OTTHUMP00000209739; OTTHUMP00000209740; OTTHUMP00000209741; OTTHUMP00000209742; OTTHUMP00000209743; OTTHUMP00000209744; p40; p51; P51/P63; p53-related protein p63; p53CP; p63; P63_HUMAN; p73H; p73L; RHS; SHFM4; TAp63alpha; TP53CP; TP53L; TP63; TP73L; Transformation related protein 63; Transformation-related protein 63; Trp53rp1; Trp63; Tumor protein 63; Tumor protein p53-competing protein; Tumor protein p53-like; Tumor protein p63; Tumor protein p63 deltaN isoform delta; Tumor protein p73; Tumor protein p73-like;
抗原和靶标
Purified recombinant fragment of human p63alpha expressed in E. Coli.
Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
- Q9H3D4 P63_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNFETSRCATLQYCPDPYIQRFVETPAHFSWKESYYRSTMSQSTQTNEFLSPEVFQHIWDFLEQPICSVQPIDLNFVDEPSEDGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSTKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTIPDGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHEFSSPSHLLRTPSSASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDARRNKQQRIKEEGE
翻译修饰 - Q9H3D4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y13 | Phosphorylation | Uniprot | |
| Y18 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| T46 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S68 | Phosphorylation | P36897 (TGFBR1) | Uniprot |
| Y149 | Phosphorylation | P00519 (ABL1) | Uniprot |
| S160 | Phosphorylation | P36897 (TGFBR1) | Uniprot |
| S162 | Phosphorylation | Uniprot | |
| Y171 | Phosphorylation | P00519 (ABL1) | Uniprot |
| Y231 | Phosphorylation | Uniprot | |
| K232 | Acetylation | Uniprot | |
| K242 | Methylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| Y290 | Phosphorylation | P00519 (ABL1) | Uniprot |
| T326 | Phosphorylation | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | P23443 (RPS6KB1) , Q13315 (ATM) , P24941 (CDK2) | Uniprot |
| S479 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T491 | Phosphorylation | Q13315 (ATM) , P24941 (CDK2) , P23443 (RPS6KB1) , Q9H2X6 (HIPK2) | Uniprot |
| S560 | Phosphorylation | Q13315 (ATM) , P23443 (RPS6KB1) , P24941 (CDK2) | Uniprot |
| K588 | Sumoylation | Uniprot | |
| K599 | Ubiquitination | Uniprot | |
| K676 | Sumoylation | Uniprot |
研究背景
Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.
May be sumoylated.
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
Nucleus.
Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L.
The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.
Belongs to the p53 family.
研究领域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
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