产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0686, RRID:AB_2833926.
展开/折叠
AGM4; B cell adapter containing SH2 domain protein; B cell adapter containing Src homology 2 domain protein; B cell adaptor containing SH2 domain; B cell linker; B cell linker protein; B cell-specific adaptor protein; B-cell activation; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; B-cell linker protein; BASH; Bca; BLNK; BLNK s; BLNK_HUMAN; Cytoplasmic adapter protein; Ly 57; Ly57; Lymphocyte antigen 57; Lyw 57; Lyw57; MGC111051; SH2 domain-containing leukocyte protein, 65-KD; SLP 65; SLP-65; SLP65; Src homology [SH2] domain-containing leukocyte protein of 65 kD; Src homology 2 domain containing leukocyte protein of 65 kDa; Src homology 2 domain-containing leukocyte protein of 65 kDa;
抗原和靶标
Purified recombinant fragment of human BLNK expressed in E. Coli.
Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
- Q8WV28 BLNK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMNKIKKLKVKAPPSVPRRDYASESPADEEEQWSDDFDSDYENPDEHSDSEMYVMPAEENADDSYEPPPVEQETRPVHPALPFARGEYIDNRSSQRHSPPFSKTLPSKPSWPSEKARLTSTLPALTALQKPQVPPKPKGLLEDEADYVVPVEDNDENYIHPTESSSPPPEKAPMVNRSTKPNSSTPASPPGTASGRNSGAWETKSPPPAAPSPLPRAGKKPTTPLKTTPVASQQNASSVCEEKPIPAERHRGSSHRQEAVQSPVFPPAQKQIHQKPIPLPRFTEGGNPTVDGPLPSFSSNSTISEQEAGVLCKPWYAGACDRKSAEEALHRSNKDGSFLIRKSSGHDSKQPYTLVVFFNKRVYNIPVRFIEATKQYALGRKKNGEEYFGSVAEIIRNHQHSPLVLIDSQNNTKDSTRLKYAVKVS
翻译修饰 - Q8WV28 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y72 | Phosphorylation | P43405 (SYK) | Uniprot |
| Y84 | Phosphorylation | P43405 (SYK) | Uniprot |
| Y96 | Phosphorylation | P43405 (SYK) | Uniprot |
| S129 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| T152 | Phosphorylation | Uniprot | |
| Y178 | Phosphorylation | P43405 (SYK) | Uniprot |
| Y189 | Phosphorylation | P43405 (SYK) | Uniprot |
| T193 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| K405 | Acetylation | Uniprot |
研究背景
Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
Cytoplasm. Cell membrane.
Note: BCR activation results in the translocation to membrane fraction.
Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
Associates with PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with VAV3, PLCG2 and GRB2. Interacts through its SH2 domain with CD79A. Interacts (via SH2 domain) with SYK; phosphorylated and activated by SYK. Interacts (via SH2 domain) with SCIMP; this interaction is dependent on phosphorylation of SCIMP 'Tyr-131'.
研究领域
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
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