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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0055, RRID:AB_2833965.
展开/折叠
c-Jun N-terminal kinase kinase 2; Dual specificity mitogen activated protein kinase kinase 7; Dual specificity mitogen-activated protein kinase kinase 7; JNK activating kinase 2; JNK kinase 2; JNK-activating kinase 2; JNKK 2; Jnkk-2; Jnkk2; MAP kinase kinase 7; MAP2K7; MAPK/ERK kinase 7; MAPKK 7; MAPKK-7; MAPKK7; MEK 7; Mitogen Activated Protein Kinase kinase 7; MKK 7; MKK-7; MKK7; MP2K7_HUMAN; PRKMK 7; PRKMK-7; PRKMK7; SAPK kinase 4; SAPKK-4; SAPKK4; Sek 2; Sek-2; Sek2; SKK4; stress-activated protein kinase kinase 4;
抗原和靶标
Purified recombinant fragment of human MAP2K7 expressed in E. Coli.
Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
- O14733 MP2K7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASSLEQKLSRLEAKLKQENREARRRIDLNLDISPQRPRPTLQLPLANDGGSRSPSSESSPQHPTPPARPRHMLGLPSTLFTPRSMESIEIDQKLQEIMKQTGYLTIGGQRYQAEINDLENLGEMGSGTCGQVWKMRFRKTGHVIAVKQMRRSGNKEENKRILMDLDVVLKSHDCPYIVQCFGTFITNTDVFIAMELMGTCAEKLKKRMQGPIPERILGKMTVAIVKALYYLKEKHGVIHRDVKPSNILLDERGQIKLCDFGISGRLVDSKAKTRSAGCAAYMAPERIDPPDPTKPDYDIRADVWSLGISLVELATGQFPYKNCKTDFEVLTKVLQEEPPLLPGHMGFSGDFQSFVKDCLTKDHRKRPKYNKLLEHSFIKRYETLEVDVASWFKDVMAKTESPRTSGVLSQPHLPFFR
翻译修饰 - O14733 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| T66 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| T83 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| S271 | Phosphorylation | Q13233 (MAP3K1) , Q9Y2U5 (MAP3K2) | Uniprot |
| T275 | Phosphorylation | Q9Y2U5 (MAP3K2) , Q13233 (MAP3K1) | Uniprot |
| S277 | Phosphorylation | Uniprot | |
| Y283 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| T401 | Phosphorylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| T406 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot |
翻译修饰 - O14733 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P35568 (IRS1) | S312 | Uniprot |
| P45983-3 (MAPK8) | T183 | Uniprot |
| P45983 (MAPK8) | Y185 | Uniprot |
| P45984-2 (MAPK9) | T183 | Uniprot |
| P45984-4 (MAPK9) | Y185 | Uniprot |
| P45984-4 (MAPK9) | T404 | Uniprot |
| P45984 (MAPK9) | S407 | Uniprot |
| P53779 (MAPK10) | T221 | Uniprot |
| Q13158 (FADD) | S194 | Uniprot |
| Q16539 (MAPK14) | Y182 | Uniprot |
研究背景
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE.
Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks).
Nucleus. Cytoplasm.
Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
Interacts with isoform 1 of VRK2. Interacts (via its D domain) with its substrates MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3 (By similarity). Interacts (via its DVD domain) with MAP3Ks activators like MAP3K5/ASK1 and MAP3K1/MEKK1 (By similarity). Interacts with MAPK8IP1/JIP1, MAPK8IP2/JIP2 and MAPK8IP3/JIP3 scaffold proteins. Interacts with RASSF7, the interaction promotes phosphorylation. Found in a complex with SH3RF1, RAC1, MAP3K11/MLK3, MAPK8IP1/JIP1 and MAPK8/JNK1. Found in a complex with SH3RF1, RAC2, MAP3K7/TAK1, MAPK8IP1/JIP1, MAPK8/JNK1 and MAPK9/JNK2 (By similarity).
The DVD domain (residues 377-400) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation.
The D domain (residues 37-57) contains a conserved docking site and is required for the binding to MAPK substrates.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Relaxin signaling pathway.
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