产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0030, RRID:AB_2834054.
展开/折叠
59 kDa serine/threonine protein kinase; 59 kDa serine/threonine-protein kinase; DKFZp686F1765; Epididymis secretory protein Li 28; HEL S 28; ILK 1; ILK 2; ILK; ILK-1; ILK-2; ILK_HUMAN; ILK1; ILK2; Integrin linked kinase 2; Integrin linked Kinase; Integrin linked protein kinase; Integrin-linked protein kinase; p59; p59ILK;
抗原和靶标
Purified recombinant fragment of human ILK expressed in E. Coli.
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
- Q13418 ILK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDIFTQCREGNAVAVRLWLDNTENDLNQGDDHGFSPLHWACREGRSAVVEMLIMRGARINVMNRGDDTPLHLAASHGHRDIVQKLLQYKADINAVNEHGNVPLHYACFWGQDQVAEDLVANGALVSICNKYGEMPVDKAKAPLRELLRERAEKMGQNLNRIPYKDTFWKGTTRTRPRNGTLNKHSGIDFKQLNFLTKLNENHSGELWKGRWQGNDIVVKVLKVRDWSTRKSRDFNEECPRLRIFSHPNVLPVLGACQSPPAPHPTLITHWMPYGSLYNVLHEGTNFVVDQSQAVKFALDMARGMAFLHTLEPLIPRHALNSRSVMIDEDMTARISMADVKFSFQCPGRMYAPAWVAPEALQKKPEDTNRRSADMWSFAVLLWELVTREVPFADLSNMEIGMKVALEGLRPTIPPGISPHVCKLMKICMNEDPAKRPKFDMIVPILEKMQDK
翻译修饰 - Q13418 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K170 | Methylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| T173 | Phosphorylation | P17252 (PRKCA) , Q13153 (PAK1) | Uniprot |
| T181 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K184 | Ubiquitination | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K191 | Sumoylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| K341 | Ubiquitination | Uniprot | |
| S343 | Phosphorylation | Q13418 (ILK) | Uniprot |
| C346 | S-Nitrosylation | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| K426 | Acetylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K448 | Ubiquitination | Uniprot |
翻译修饰 - Q13418 作为激酶
| Substrate | Site | Source |
|---|---|---|
| E9PAV3 (NACA) | S1906 | Uniprot |
| O14950 (MYL12B) | T19 | Uniprot |
| O14950 (MYL12B) | S20 | Uniprot |
| O14974-1 (PPP1R12A) | T696 | Uniprot |
| O14974 (PPP1R12A) | T710 | Uniprot |
| P23528 (CFL1) | S3 | Uniprot |
| P31749 (AKT1) | S473 | Uniprot |
| Q13418-1 (ILK) | S343 | Uniprot |
| Q13765 (NACA) | S43 | Uniprot |
| Q8TAE6 (PPP1R14C) | T73 | Uniprot |
| Q96A00 (PPP1R14A) | T38 | Uniprot |
| Q96C90 (PPP1R14B) | T57 | Uniprot |
研究背景
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Autophosphorylated on serine residues.
Cell junction>Focal adhesion. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium. Cytoplasm>Myofibril>Sarcomere.
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Interacts with FERMT2 (By similarity). Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with PARVA and PARVB; these compete for the same binding site. Interacts probably also with TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Interacts with LIMD2; leading to activate the protein kinase activity.
A PH-like domain is involved in phosphatidylinositol phosphate binding.
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Cancers: Specific types > Endometrial cancer. (View pathway)
· Organismal Systems > Endocrine system > PPAR signaling pathway.
· Organismal Systems > Development > Axon guidance. (View pathway)
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