STAT5A Antibody | Affinity Biosciences LTD|亲科生物官网

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Figure 1: Western blot analysis using STAT5A mAb against human STAT5A recombinant protein.

产品:	STAT5A 抗体
货号:	BF0022
描述:	Mouse monoclonal antibody to STAT5A
应用:	WB IHC IF/ICC ELISA FACS
反应:	Human
分子量:	90.6kDa; 91kD(Calculated).
蛋白号:	P42229
RRID:	AB_2834061

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规格	价格	库存
50ul	RMB¥ 1800	现货
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实验方案

产品描述

来源:

Mouse

应用:

ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human

克隆:

Monoclonal [AFB2039]

特异性:

STAT5A antibody detects endogenous levels of total STAT5A.

RRID:

AB_2834061
引用格式: Affinity Biosciences Cat# BF0022, RRID:AB_2834061.

偶联:

Unconjugated.

纯化:

Affinity-chromatography.

保存:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Mammary gland factor; MGF; Signal transducer and activator of transcription 5A; STA5A_HUMAN; STAT 5; STAT 5A; STAT5; STAT5A;

抗原和靶标

免疫原:

Purified recombinant fragment of human STAT5A expressed in E. Coli.

Uniprot:

P42229(STA5A_HUMAN) >>Visit HPA database.

基因/基因ID:

STAT5A(6776)

描述:

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.

序列:

P42229 STA5A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGWIQAQQLQGDALRQMQVLYGQHFPIEVRHYLAQWIESQPWDAIDLDNPQDRAQATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQKTYDRCPLELVRCIRHILYNEQRLVREANNCSSPAGILVDAMSQKHLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFAQLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNECSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRADRRGAESVTEEKFTVLFESQFSVGSNELVFQVKTLSLPVVVIVHGSQDHNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGWNYTFWQWFDGVMEVLKKHHKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSPERNLWNLKPFTTRDFSIRSLADRLGDLSYLIYVFPDRPKDEVFSKYYTPVLAKAVDGYVKPQIKQVVPEFVNASADAGGSSATYMDQAPSPAVCPQAPYNMYPQNPDHVLDQDGEFDLDETMDVARHVEELLRRPMDSLDSRLSPPAGLFTSARGSLS

翻译修饰 - P42229 作为底物

P42229

Site	PTM Type	Enzyme	Source
Y22	Phosphorylation		Uniprot
K70	Ubiquitination		Uniprot
K71	Ubiquitination		Uniprot
K84	Acetylation		Uniprot
K84	Ubiquitination		Uniprot
K86	Ubiquitination		Uniprot
Y90	Phosphorylation		Uniprot
T92	O-Glycosylation		Uniprot
T92	Phosphorylation		Uniprot
Y114	Phosphorylation		Uniprot
S127	Phosphorylation		Uniprot
S128	Phosphorylation		Uniprot
K163	Ubiquitination		Uniprot
K164	Ubiquitination		Uniprot
S193	Phosphorylation		Uniprot
K207	Ubiquitination		Uniprot
K232	Ubiquitination		Uniprot
K235	Ubiquitination		Uniprot
K242	Ubiquitination		Uniprot
T245	Phosphorylation		Uniprot
K256	Sumoylation		Uniprot
K256	Ubiquitination		Uniprot
K336	Sumoylation		Uniprot
K343	Ubiquitination		Uniprot
K359	Ubiquitination		Uniprot
K370	Ubiquitination		Uniprot
T372	Phosphorylation		Uniprot
S375	Phosphorylation		Uniprot
K380	Ubiquitination		Uniprot
K384	Acetylation		Uniprot
K384	Sumoylation		Uniprot
K384	Ubiquitination		Uniprot
S473	Phosphorylation		Uniprot
K516	Sumoylation		Uniprot
K516	Ubiquitination		Uniprot
K527	Ubiquitination		Uniprot
K586	Ubiquitination		Uniprot
K644	Ubiquitination		Uniprot
T647	Phosphorylation		Uniprot
T648	Phosphorylation		Uniprot
Y668	Phosphorylation		Uniprot
K675	Ubiquitination		Uniprot
K681	Ubiquitination		Uniprot
Y682	Phosphorylation		Uniprot
Y683	Phosphorylation		Uniprot
K689	Ubiquitination		Uniprot
Y694	Phosphorylation	Q06187 (BTK) , P12931 (SRC) , P52333 (JAK3) , P06239 (LCK) , P06213 (INSR) , P07948 (LYN) , P43405 (SYK) , Q13882 (PTK6) , O60674 (JAK2) , P08631 (HCK)	Uniprot
K696	Acetylation		Uniprot
K696	Sumoylation		Uniprot
K696	Ubiquitination		Uniprot
K700	Acetylation		Uniprot
Y720	Phosphorylation		Uniprot
S726	Phosphorylation		Uniprot
T757	Phosphorylation		Uniprot
S774	Phosphorylation		Uniprot
S777	Phosphorylation		Uniprot
S780	Phosphorylation	Q13153 (PAK1) , P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
S792	Phosphorylation		Uniprot
S794	Phosphorylation		Uniprot

研究背景

P42229_STA5A_HUMAN

功能:

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

翻译修饰:

Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO (By similarity). Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694.

ISGylated.

细胞定位:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.

亚基结构:

Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7. Interacts with ERBB4.

蛋白家族:

Belongs to the transcription factor STAT family.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)

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STAT5A Antibody - #BF0022

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