(3)
(8)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0589, RRID:AB_2834263.
展开/折叠
AT225;Early growth response 1;Early growth response protein 1;EGR 1;EGR-1;EGR1;EGR1_HUMAN;G0S30;KROX 24;KROX24;Nerve growth factor-induced clone A;Nerve growth factor-induced protein A;NGFI-A;NGFIA;TIS8;Transcription factor ETR103;Transcription factor Zif268;ZIF 268;ZIF268;Zinc finger protein 225;Zinc finger protein Krox-24;ZNF225;
抗原和靶标
A synthesized peptide derived from human EGR1, corresponding to a region within the internal amino acids.
- P18146 EGR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAKAEMQLMSPLQISDPFGSFPHSPTMDNYPKLEEMMLLSNGAPQFLGAAGAPEGSGSNSSSSSSGGGGGGGGGSNSSSSSSTFNPQADTGEQPYEHLTAESFPDISLNNEKVLVETSYPSQTTRLPPITYTGRFSLEPAPNSGNTLWPEPLFSLVSGLVSMTNPPASSSSAPSPAASSASASQSPPLSCAVPSNDSSPIYSAAPTFPTPNTDIFPEPQSQAFPGSAGTALQYPPPAYPAAKGGFQVPMIPDYLFPQQQGDLGLGTPDQKPFQGLESRTQQPSLTPLSTIKAFATQSGSQDLKALNTSYQSQLIKPSRMRKYPNRPSKTPPHERPYACPVESCDRRFSRSDELTRHIRIHTGQKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACDICGRKFARSDERKRHTKIHLRQKDKKADKSVVASSATSSLSSYPSPVATSYPSPVTTSYPSPATTSYPSPVPTSFSSPGSSTYPSPVHSGFPSPSVATTYSSVPPAFPAQVSSFPSSAVTNSFSASTGLSDMTATFSPRTIEIC
翻译修饰 - P18146 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S120 | O-Glycosylation | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K317 | Acetylation | Uniprot | |
| S378 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| T389 | Phosphorylation | Uniprot | |
| T391 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| T526 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
研究背景
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (By similarity). Binds double-stranded target DNA, irrespective of the cytosine methylation status. Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (By similarity). Regulates the amplitude of the expression rhythms of clock genes: ARNTL/BMAL1, PER2 and NR1D1 in the liver via the activation of PER1 (clock repressor) transcription. Regulates the rhythmic expression of core-clock gene ARNTL/BMAL1 in the suprachiasmatic nucleus (SCN) (By similarity).
Nucleus. Cytoplasm.
Detected in neutrophils (at protein level).
Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction.
Binds to DNA motifs with the sequence 5'-GCG(T/G)GGGCG-3' via its C2H2-type zinc fingers (PubMed:25258363, PubMed:25999311). The first, most N-terminal zinc finger binds to the 3'-GCG motif, the middle zinc finger interacts with the central TGG motif, and the C-terminal zinc finger binds to the 5'-GCG motif. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Has reduced affinity for target DNA where the cytosines have been oxidized to 5-hydroxymethylcytosine. Does not bind target DNA where the cytosines have been oxidized to 5-formylcytosine or 5-carboxylcytosine (PubMed:25258363).
Belongs to the EGR C2H2-type zinc-finger protein family.
研究领域
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Neurodegenerative diseases > Prion diseases.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
文献引用
Application: WB Species: Human Sample: CAL-62 cells
Application: WB Species: human Sample: CAL-62 cells;BHT-101 cells
Application: WB Species: Human Sample: thyroid papillary carcinoma cells
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