产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0596, RRID:AB_2834282.
展开/折叠
dJ862P8.3; KIAA1804; M3KL4_HUMAN; Mitogen activated protein kinase kinase kinase; Mitogen-activated protein kinase kinase kinase MLK4; Mixed lineage kinase 4; MLK 4; Mlk4; RP5 862P8.2;
抗原和靶标
- Q5TCX8 M3K21_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALRGAAGATDTPVSSAGGAPGGSASSSSTSSGGSASAGAGLWAALYDYEARGEDELSLRRGQLVEVLSQDAAVSGDEGWWAGQVQRRLGIFPANYVAPCRPAASPAPPPSRPSSPVHVAFERLELKELIGAGGFGQVYRATWQGQEVAVKAARQDPEQDAAAAAESVRREARLFAMLRHPNIIELRGVCLQQPHLCLVLEFARGGALNRALAAANAAPDPRAPGPRRARRIPPHVLVNWAVQIARGMLYLHEEAFVPILHRDLKSSNILLLEKIEHDDICNKTLKITDFGLAREWHRTTKMSTAGTYAWMAPEVIKSSLFSKGSDIWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIPSTCPEPFAKLMKECWQQDPHIRPSFALILEQLTAIEGAVMTEMPQESFHSMQDDWKLEIQQMFDELRTKEKELRSREEELTRAALQQKSQEELLKRREQQLAEREIDVLERELNILIFQLNQEKPKVKKRKGKFKRSRLKLKDGHRISLPSDFQHKITVQASPNLDKRRSLNSSSSSPPSSPTMMPRLRAIQLTSDESNKTWGRNTVFRQEEFEDVKRNFKKKGCTWGPNSIQMKDRTDCKERIRPLSDGNSPWSTILIKNQKTMPLASLFVDQPGSCEEPKLSPDGLEHRKPKQIKLPSQAYIDLPLGKDAQRENPAEAESWEEAASANAATVSIEMTPTNSLSRSPQRKKTESALYGCTVLLASVALGLDLRELHKAQAAEEPLPKEEKKKREGIFQRASKSRRSASPPTSLPSTCGEASSPPSLPLSSALGILSTPSFSTKCLLQMDSEDPLVDSAPVTCDSEMLTPDFCPTAPGSGREPALMPRLDTDCSVSRNLPSSFLQQTCGNVPYCASSKHRPSHHRRTMSDGNPTPTGATIISATGASALPLCPSPAPHSHLPREVSPKKHSTVHIVPQRRPASLRSRSDLPQAYPQTAVSQLAQTACVVGRPGPHPTQFLAAKERTKSHVPSLLDADVEGQSRDYTVPLCRMRSKTSRPSIYELEKEFLS
翻译修饰 - Q5TCX8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y47 | Phosphorylation | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K454 | Ubiquitination | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| S517 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| T592 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| T604 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| T622 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| Y669 | Phosphorylation | Uniprot | |
| T719 | Phosphorylation | Uniprot | |
| Y724 | Phosphorylation | Uniprot | |
| S768 | Phosphorylation | Uniprot | |
| S773 | Phosphorylation | Uniprot | |
| S895 | Phosphorylation | Uniprot | |
| S920 | Phosphorylation | Uniprot | |
| S932 | Phosphorylation | Uniprot | |
| S949 | Phosphorylation | Uniprot | |
| S1026 | Phosphorylation | Uniprot |
研究背景
Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
Homodimer (By similarity). Interacts with TLR4.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
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