产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0574, RRID:AB_2834285.
展开/折叠
alpha-induced protein 9; Metalloreductase STEAP4; six transmembrane prostate protein 2; Six-transmembrane epithelial antigen of prostate 4; SixTransMembrane protein of prostate 2; STAMP 2; STAMP2; STEA4_HUMAN; STEAP 4; STEAP family member 4; STEAP4; TIARP; TNFAIP 9; TNFAIP9; Tumor necrosis factor; tumor necrosis factor, alpha-induced protein 9; tumor necrosis-alpha-induced adipose-related protein;
抗原和靶标
Ubiquitous. Highly expressed in adipose tissue. Expressed in placenta, lung, heart and prostate. Detected at lower levels in liver, skeletal muscle, pancreas, testis and small intestine. Highly expressed in joints of patients with rheumatoid arthritis and localized with CD68 cells, a marker for macrophages.
- Q687X5 STEA4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEKTCIDALPLTMNSSEKQETVCIFGTGDFGRSLGLKMLQCGYSVVFGSRNPQKTTLLPSGAEVLSYSEAAKKSGIIIIAIHREHYDFLTELTEVLNGKILVDISNNLKINQYPESNAEYLAHLVPGAHVVKAFNTISAWALQSGALDASRQVFVCGNDSKAKQRVMDIVRNLGLTPMDQGSLMAAKEIEKYPLQLFPMWRFPFYLSAVLCVFLFFYCVIRDVIYPYVYEKKDNTFRMAISIPNRIFPITALTLLALVYLPGVIAAILQLYRGTKYRRFPDWLDHWMLCRKQLGLVALGFAFLHVLYTLVIPIRYYVRWRLGNLTVTQAILKKENPFSTSSAWLSDSYVALGILGFFLFVLLGITSLPSVSNAVNWREFRFVQSKLGYLTLILCTAHTLVYGGKRFLSPSNLRWYLPAAYVLGLIIPCTVLVIKFVLIMPCVDNTLTRIRQGWERNSKH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q687X5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K3 | Ubiquitination | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| Y225 | Phosphorylation | Uniprot | |
| N323 | N-Glycosylation | Uniprot | |
| S408 | Phosphorylation | Uniprot |
研究背景
Integral membrane protein that functions as NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane. Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate. Can also reduce Cu(2+) to Cu(1+) (By similarity). Plays a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses (By similarity). Associated with obesity and insulin-resistance. Involved in inflammatory arthritis, through the regulation of inflammatory cytokines. Inhibits anchorage-independent cell proliferation.
Cell membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Early endosome membrane>Multi-pass membrane protein.
Ubiquitous. Highly expressed in adipose tissue. Expressed in placenta, lung, heart and prostate. Detected at lower levels in liver, skeletal muscle, pancreas, testis and small intestine. Highly expressed in joints of patients with rheumatoid arthritis and localized with CD68 cells, a marker for macrophages.
Homotrimer. Interacts with PTK2/FAK1; the interaction may regulate PTK2 phosphorylation.
Belongs to the STEAP family.
限制条款
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