MAST2 Antibody - #AF0571

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产品: MAST2 抗体
货号: AF0571
描述: Rabbit polyclonal antibody to MAST2
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 196kDa; 196kD(Calculated).
蛋白号: Q6P0Q8
RRID: AB_2834288

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
MAST2 Antibody detects endogenous levels of total MAST2.
RRID:
AB_2834288
引用格式: Affinity Biosciences Cat# AF0571, RRID:AB_2834288.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 2.7.11.1 3; FLJ39200; KIAA0807; MAST 2; MAST 205; Mast2; MAST2_HUMAN; Microtubule associated serine/threonine kinase 2; Microtubule associated testis specific serine/threonine protein kinase; Microtubule-associated serine/threonine-protein kinase 2; MTSSK; OTTHUMP00000009697 2; OTTHUMP00000046616; RP4 533D7.1; RP4-533D7.1 2;

抗原和靶标

免疫原:
Uniprot:

Q6P0Q8(MAST2_HUMAN) >>Visit HPA database.

基因/基因ID:
MAST2(23139)
表达:
Q6P0Q8 MAST2_HUMAN:

Abundant in the testis.

描述:
MAST2 appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappaB activation. 2 isoforms of the human protein are produced by alternative splicing.
序列:
MKRSRCRDRPQPPPPDRREDGVQRAAELSQSLPPRRRAPPGRQRLEERTGPAGPEGKEQDVVTGVSPLLFRKLSNPDIFSSTGKVKLQRQLSQDDCKLWRGNLASSLSGKQLLPLSSSVHSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFVPARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADELHFLTKHFSTESVPDEEGRQSPAMRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEERLAEFISSNTPDSVLPLADGALSFIHHQVIEMARDCLDKSRSGLITSQYFYELQDNLEKLLQDAHERSESSEVAFVMQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLTRDPLEEMAQLSSCDSPDTPETDDSIEGHGASLPSKKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFDTKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDEALPPDAQDLTSKLLHQNPLERLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHMDSEDEEEVSEDGCLEIRQFSSCSPRFNKVYSSMERLSLLEERRTPPPTKRSLSEEKEDHSDGLAGLKGRDRSWVIGSPEILRKRLSVSESSHTESDSSPPMTVRRRCSGLLDAPRFPEGPEEASSTLRRQPQEGIWVLTPPSGEGVSGPVTEHSGEQRPKLDEEAVGRSSGSSPAMETRGRGTSQLAEGATAKAISDLAVRRARHRLLSGDSTEKRTARPVNKVIKSASATALSLLIPSEHHTCSPLASPMSPHSQSSNPSSRDSSPSRDFLPALGSMRPPIIIHRAGKKYGFTLRAIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVELILKSGNKVAISTTPLENTSIKVGPARKGSYKAKMARRSKRSRGKDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSHSLSPRSPTQGYRVTPDAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPPTASPQRSPSPLSGHVAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASEKKLATSRKHSLDLPHSELKKELPPREVSPLEVVGARSVLSGKGALPGKGVLQPAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTEEGPENSQGAQELSLAPHPEVSQSVAPKGAGESGEEDPFPSRDPRSLGPMVPSLLTGITLGPPRMESPSGPHRRLGSPQAIEEAASSSSAGPNLGQSGATDPIPPEGCWKAQHLHTQALTALSPSTSGLTPTSSCSPPSSTSGKLSMWSWKSLIEGPDRASPSRKATMAGGLANLQDLENTTPAQPKNLSPREQGKTQPPSAPRLAHPSYEDPSQGWLWESECAQAVKEDPALSITQVPDASGDRRQDVPCRGCPLTQKSEPSLRRGQEPGGHQKHRDLALVPDELLKQT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
91
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q6P0Q8 作为底物

Site PTM Type Enzyme
T63 Phosphorylation
S66 Phosphorylation
S74 Phosphorylation
S81 Phosphorylation
T82 Phosphorylation
S92 Phosphorylation
S108 Phosphorylation
S144 Phosphorylation
S148 Phosphorylation
S151 Phosphorylation
S160 Phosphorylation
R163 Methylation
R169 Methylation
S183 Phosphorylation
T185 Phosphorylation
S191 Phosphorylation
S204 Phosphorylation
S209 Phosphorylation
S230 Phosphorylation
T243 Phosphorylation
T274 Phosphorylation
S278 Phosphorylation
T279 Phosphorylation
S281 Phosphorylation
S290 Phosphorylation
S297 Phosphorylation
S299 Phosphorylation
S301 Phosphorylation
S305 Phosphorylation
K455 Ubiquitination
Y461 Phosphorylation
S519 Phosphorylation
S533 Phosphorylation
T534 Phosphorylation
K545 Ubiquitination
S657 Phosphorylation
K683 Ubiquitination
K797 Ubiquitination
S806 Phosphorylation
Y812 Phosphorylation
S825 Phosphorylation
S846 Phosphorylation
Y853 Phosphorylation
S854 Phosphorylation
S855 Phosphorylation
S860 Phosphorylation
T867 Phosphorylation
T871 Phosphorylation
S874 Phosphorylation
S876 Phosphorylation
S883 Phosphorylation
K890 Ubiquitination
S895 Phosphorylation
S900 Phosphorylation
S920 Phosphorylation
S931 Phosphorylation
S993 Phosphorylation
S995 Phosphorylation
S996 Phosphorylation
R1004 Methylation
S1007 Phosphorylation
S1032 Phosphorylation
S1035 Phosphorylation
S1180 Phosphorylation
K1183 Ubiquitination
S1195 Phosphorylation
K1197 Ubiquitination
K1203 Acetylation
K1207 Acetylation
K1209 Acetylation
K1237 Ubiquitination
S1240 Phosphorylation
S1245 Phosphorylation
S1247 Phosphorylation
S1250 Phosphorylation
S1256 Phosphorylation
S1264 Phosphorylation
S1275 Phosphorylation
S1337 Phosphorylation P28482 (MAPK1)
S1340 Phosphorylation
S1344 Phosphorylation
T1349 Phosphorylation
S1351 Phosphorylation
S1364 Phosphorylation
S1381 Phosphorylation
S1393 Phosphorylation
S1399 Phosphorylation
K1403 Methylation
S1407 Phosphorylation
S1425 Phosphorylation
S1429 Phosphorylation
S1435 Phosphorylation
S1447 Phosphorylation
S1503 Phosphorylation
S1504 Phosphorylation
T1508 Phosphorylation
S1541 Phosphorylation
S1554 Phosphorylation
T1564 Phosphorylation
T1567 Phosphorylation
S1585 Phosphorylation
S1631 Phosphorylation
S1642 Phosphorylation
K1659 Methylation
S1660 Phosphorylation
S1669 Phosphorylation
S1698 Phosphorylation
S1717 Phosphorylation
S1768 Phosphorylation
S1771 Phosphorylation
T1798 Phosphorylation

研究背景

功能:

Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation (By similarity).

翻译修饰:

Phosphorylated and ubiquitinated. N-terminal ubiquitination leads to degradation of MAST2 by proteasome-mediated proteolysis. N-terminal phosphorylation appears to be a prerequisite for ubiquitination (By similarity).

细胞定位:

Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Recruited to the sub-membranous area on interaction with CDHR2.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Abundant in the testis.

亚基结构:

Interacts with CDHR2.

蛋白家族:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

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