DOK3 Antibody - #AF0600

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(1)   (5)  
产品: DOK3 抗体
货号: AF0600
描述: Rabbit polyclonal antibody to DOK3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Rabbit, Dog
分子量: 58kDa; 53kD(Calculated).
蛋白号: Q7L591
RRID: AB_2834292

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
DOK3 Antibody detects endogenous levels of total DOK3.
RRID:
AB_2834292
引用格式: Affinity Biosciences Cat# AF0600, RRID:AB_2834292.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Docking protein 3; DOK 3; DOK Like Protein; Dok3; DOK3_HUMAN; DOKL Pending; Downstream of tyrosine kinase 3; p62 DOK Like Protein;

抗原和靶标

免疫原:
Uniprot:

Q7L591(DOK3_HUMAN) >>Visit HPA database.

基因/基因ID:
DOK3(79930)
表达:
Q7L591 DOK3_HUMAN:

Expressed in spleen.

描述:
Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP. May modulate Abl function. On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP. 4 isoforms of the human protein are produced by alternative splicing.
序列:
MTRGARLRSDARAQLNQLSLDGGTGSGQKGKCEEFPSSLSSVSPGLEAAALLLAVTMDPLETPIKDGILYQQHVKFGKKCWRKVWALLYAGGPSGVARLESWEVRDGGLGAAGDRSAGPGRRGERRVIRLADCVSVLPADGESCPRDTGAFLLTTTERSHLLAAQHRQAWMGPICQLAFPGTGEASSGSTDAQSPKRGLVPMEENSIYSSWQEVGEFPVVVQRTEAATRCQLKGPALLVLGPDAIQLREAKGTQALYSWPYHFLRKFGSDKGVFSFEAGRRCHSGEGLFAFSTPCAPDLCRAVAGAIARQRERLPELTRPQPCPLPRATSLPSLDTPGELREMPPGPEPPTSRKMHLAEPGPQSLPLLLGPEPNDLASGLYASVCKRASGPPGNEHLYENLCVLEASPTLHGGEPEPHEGPGSRSPTTSPIYHNGQDLSWPGPANDSTLEAQYRRLLELDQVEGTGRPDPQAGFKAKLVTLLSRERRKGPAPCDRP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Chicken
70
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q7L591 作为底物

Site PTM Type Enzyme
S9 Phosphorylation
Y89 Phosphorylation
R122 Methylation
S194 Phosphorylation
Y208 Phosphorylation
T329 Phosphorylation
S330 Phosphorylation
S333 Phosphorylation
T336 Phosphorylation
T351 Phosphorylation
S352 Phosphorylation
S364 Phosphorylation
S378 Phosphorylation
Y381 Phosphorylation
S389 Phosphorylation
Y398 Phosphorylation
S407 Phosphorylation
Y432 Phosphorylation
Y453 Phosphorylation
K477 Acetylation
T480 Phosphorylation
S483 Phosphorylation

研究背景

功能:

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function (By similarity).

翻译修饰:

Constitutively tyrosine-phosphorylated.

On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase (By similarity).

细胞定位:

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in spleen.

亚基结构:

On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL1 through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP (By similarity).

蛋白家族:

PTB domain mediates receptor interaction.

Belongs to the DOK family. Type A subfamily.

文献引用

1). Dok3 is involved in cisplatin-induced acute kidney injury via regulation of inflammation and apoptosis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 34245977) [IF=3.1]

Application: IHC    Species: Mouse    Sample: Kidney tissues

Fig.1. Deletion of Dok3 attenuates cisplatin-induced AKI. (A) Level of BUN in the serum. (A) Level of creatinine in the serum; (C) Images of immunohistochemical staining of NGAL; 400 ? magnification. (D) Quantification of immunohistochemical staining of NGAL. (E) Analysis of survival rate of mice. All values are expressed as the mean ± standard error. n ¼ 6, **P < 0.01, and ***P < 0.001.

Application: WB    Species: Mouse    Sample: Kidney tissues

Fig. 4. Overexpression of DOK3 exacerbates apoptosis and inflammation in vitro. (A) Dok3 mRNA expression in mouse kidney tissues. (B) Dok3 protein levels in mouse kidney tissues. (C) Images of immunohistochemical (IHC) staining of DOK3 (400 ? magnification). (D) Images of immunofluorescence staining of DOK3/AQP1 (400 ? magnification). (E) Dok3 protein levels in HK2 cells. (F) Western blot analysis verifying pcDNA-Dok3 expression. (G) PI-Hochest staining of pcDNA-Dok3-transfected HK2 cells with/without cisplatin treatment. (H) LDH level in the cell culture supernatant. (I) TNF- a level in cell culture supernatant. (J) IL-6 level in cell culture supernatant. All values are expressed as the mean ± standard error, *P < 0.05, **P < 0.01, and ***P < 0.001.

Application: IF/ICC    Species: Mouse    Sample: Kidney tissues

Fig. 4. Overexpression of DOK3 exacerbates apoptosis and inflammation in vitro. (A) Dok3 mRNA expression in mouse kidney tissues. (B) Dok3 protein levels in mouse kidney tissues. (C) Images of immunohistochemical (IHC) staining of DOK3 (400 ? magnification). (D) Images of immunofluorescence staining of DOK3/AQP1 (400 ? magnification). (E) Dok3 protein levels in HK2 cells. (F) Western blot analysis verifying pcDNA-Dok3 expression. (G) PI-Hochest staining of pcDNA-Dok3-transfected HK2 cells with/without cisplatin treatment. (H) LDH level in the cell culture supernatant. (I) TNF- a level in cell culture supernatant. (J) IL-6 level in cell culture supernatant. All values are expressed as the mean ± standard error, *P < 0.05, **P < 0.01, and ***P < 0.001.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.