产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
ALPK2 Antibody detects endogenous levels of total ALPK2.
RRID:
AB_2834296
引用格式: Affinity Biosciences Cat# AF0666, RRID:AB_2834296.
引用格式: Affinity Biosciences Cat# AF0666, RRID:AB_2834296.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
Alpha-protein kinase 2; ALPK2; ALPK2_HUMAN; FLJ34875; FLJ43253; Gm549; HAK; Heart alpha-protein kinase; RGD1564431;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
表达:
描述:
AlphaK2 Kinases that recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation. Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily. 2 isoforms of the human protein are produced by alternative splicing.
序列:
- Q86TB3 ALPK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKDSEGPQRPPLCFLSTLLSQKVPEKSDAVLRCIISGQPKPEVTWYKNGQAIDGSGIISNYEFFENQYIHVLHLSCCTKNDAAVYQISAKNSFGMICCSASVEVECSSENPQLSPNLEDDRDRGWKHETGTHEEERANQIDEKEHPYKEEESISPGTPRSADSSPSKSNHSLSLQSLGNLDISVSSSENPLGVKGTRHTGEAYDPSNTEEIANGLLFLNSSHIYEKQDRCCHKTVHSMASKFTDGDLNNDGPHDEGLRSSQQNPKVQKYISFSLPLSEATAHIYPGDSAVANKQPSPQLSSEDSDSDYELCPEITLTYTEEFSDDDLEYLECSDVMTDYSNAVWQRNLLGTEHVFLLESDDEEMEFGEHCLGGCEHFLSGMGCGSRVSGDAGPMVATAGFCGHHSQPQEVGVRSSRVSKHGPSSPQTGMTLILGPHQDGTSSVTEQGRYKLPTAPEAAENDYPGIQGETRDSHQAREEFASDNLLNMDESVRETEMKLLSGESENSGMSQCWETAADKRVGGKDLWSKRGSRKSARVRQPGMKGNPKKPNANLRESTTEGTLHLCSAKESAEPPLTQSDKRETSHTTAAATGRSSHADARECAISTQAEQEAKTLQTSTDSVSKEGNTNCKGEGMQVNTLFETSQVPDWSDPPQVQVQETVRETISCSQMPAFSEPAGEESPFTGTTTISFSNLGGVHKENASLAQHSEVKPCTCGPQHEEKQDRDGNIPDNFREDLKYEQSISEANDETMSPGVFSRHLPKDARADFREPVAVSVASPEPTDTALTLENVCDEPRDREAVCAMECFEAGDQGTCFDTIDSLVGRPVDKYSPQEICSVDTELAEGQNKVSDLCSSNDKTLEVFFQTQVSETSVSTCKSSKDGNSVMSPLFTSTFTLNISHTASEGATGENLAKVENSTYPLASTVHAGQEQPSPSNSGGLDETQLLSSENNPLVQFKEGGDKSPSPSAADTTATPASYSSIVSFPWEKPTTLTANNECFQATRETEDTSTVTIATEVHPAKYLAVSIPEDKHAGGTEERFPRASHEKVSQFPSQVQLDHILSGATIKSTKELLCRAPSVPGVPHHVLQLPEGEGFCSNSPLQVDNLSGDKSQTVDRADFRSYEENFQERGSETKQGVQQQSLSQQGSLSAPDFQQSLPTTSAAQEERNLVPTAHSPASSREGAGQRSGWGTRVSVVAETAGEEDSQALSNVPSLSDILLEESKEYRPGNWEAGNKLKIITLEASASEIWPPRQLTNSESKASDGGLIIPDKVWAVPDSLKADAVVPELAPSEIAALAHSPEDAESALADSRESHKGEEPTISVHWRSLSSRGFSQPRLLESSVDPVDEKELSVTDSLSAASETGGKENVNNVSQDQEEKQLKMDHTAFFKKFLTCPKILESSVDPIDEISVIEYTRAGKPEPSETTPQGAREGGQSNDGNMGHEAEIQPAILQVPCLQGTILSENRISRSQEGSMKQEAEQIQPEEAKTAIWQVLQPSEGGERIPSGCSIGQIQESSDGSLGEAEQSKKDKAELISPTSPLSSCLPIMTHASLGVDTHNSTGQIHDVPENDIVEPRKRQYVFPVSQKRGTIENERGKPLPSSPDLTRFPCTSSPEGNVTDFLISHKMEEPKIEVLQIGETKPPSSSSSSAKTLAFISGERELEKAPKLLQDPCQKGTLGCAKKSREREKSLEARAGKSPGTLTAVTGSEEVKRKPEAPGSGHLAEGVKKKILSRVAALRLKLEEKENIRKNSAFLKKMPKLETSLSHTEEKQDPKKPSCKREGRAPVLLKKIQAEMFPEHSGNVKLSCQFAEIHEDSTICWTKDSKSIAQVQRSAGDNSTVSFAIVQASPKDQGLYYCCIKNSYGKVTAEFNLTAEVLKQLSSRQDTKGCEEIEFSQLIFKEDFLHDSYFGGRLRGQIATEELHFGEGVHRKAFRSTVMHGLMPVFKPGHACVLKVHNAIAYGTRNNDELIQRNYKLAAQECYVQNTARYYAKIYAAEAQPLEGFGEVPEIIPIFLIHRPENNIPYATVEEELIGEFVKYSIRDGKEINFLRRESEAGQKCCTFQHWVYQKTSGCLLVTDMQGVGMKLTDVGIATLAKGYKGFKGNCSMTFIDQFKALHQCNKYCKMLGLKSLQNNNQKQKQPSIGKSKVQTNSMTIKKAGPETPGEKKT
翻译修饰 - Q86TB3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K528 | Acetylation | Uniprot | |
| T617 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| S1078 | Phosphorylation | Uniprot | |
| K1260 | Ubiquitination | Uniprot | |
| K1419 | Ubiquitination | Uniprot | |
| S1602 | Phosphorylation | Uniprot | |
| K1697 | Ubiquitination | Uniprot | |
| K1712 | Ubiquitination | Uniprot | |
| K1728 | Ubiquitination | Uniprot | |
| K1750 | Ubiquitination | Uniprot | |
| K1826 | Ubiquitination | Uniprot | |
| K1861 | Ubiquitination | Uniprot | |
| T1868 | Phosphorylation | Uniprot | |
| Y1975 | Phosphorylation | Uniprot | |
| K1976 | Ubiquitination | Uniprot | |
| K2046 | Ubiquitination | Uniprot | |
| T2089 | Phosphorylation | Uniprot | |
| T2095 | Phosphorylation | Uniprot | |
| K2098 | Ubiquitination | Uniprot | |
| K2123 | Ubiquitination | Uniprot | |
| K2131 | Ubiquitination | Uniprot | |
| K2141 | Ubiquitination | Uniprot |
研究背景
功能:
Protein kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation. Regulates cardiac development and cardiomyocyte differentiation by negatively regulating Wnt/beta-catenin signaling.
细胞定位:
Basolateral cell membrane.
组织特异性:
Expressed in developing cardiac tissue and cardiomyocytes (at protein level).
蛋白家族:
Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
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