产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0611, RRID:AB_2834300.
展开/折叠
DAG kinase eta; DGK eta; Diacylglycerol kinase eta; Diglyceride kinase eta;
抗原和靶标
Isoform 2 is ubiquitously expressed. Isoform 1 is expressed only in testis, kidney and colon.
- Q86XP1 DGKH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGAGGQHHPPGAAGGAAAGAGAAVTSAAASAGPGEDSSDSEAEQEGPQKLIRKVSTSGQIRTKTSIKEGQLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAEASTKNANNSFTIITPFRRLMLCAENRKEMEDWISSLKSVQTREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWKCLWCKTMVHTACKDLYHPICPLGQCKVSIIPPIALNSTDSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLNLNKQCQLGVLPLGTGNDLARVLGWGGSYDDDTQLPQILEKLERASTKMLDRWSIMTYELKLPPKASLLPGPPEASEEFYMTIYEDSVATHLTKILNSDEHAVVISSAKTLCETVKDFVAKVEKTYDKTLENAVVADAVASKCSVLNEKLEQLLQALHTDSQAAPVLPGLSPLIVEEDAVESSSEESLGESKEQLGDDVTKPSSQKAVKPREIMLRANSLKKAVRQVIEEAGKVMDDPTVHPCEPANQSSDYDSTETDESKEEAKDDGAKESITVKTAPRSPDARASYGHSQTDSVPGPAVAASKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPFGATPFIDPDLDSVDGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFAAPSFDDKILEVVAIFDSMQMAVSRVIKLQHHRIAQCRTVKITIFGDEGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTHLYIHHAIDLATEEVSQMQLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANPRCPESLTRDTATEIAINVKALYNETESLLVGRVPLQLESPHEERVSNALHSVEVELQKLTEIPWLYYILHPNEDEEPPMDCTKRNNRSTVFRIVPKFKKEKVQKQKTSSQPVQKWGTEEVAAWLDLLNLGEYKDIFIRHDIRGAELLHLERRDLKDLGIPKVGHVKRILQGIKELGRSTPQSEV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q86XP1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S56 | Phosphorylation | Uniprot | |
| T57 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K266 | Methylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| K408 | Ubiquitination | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| T533 | Phosphorylation | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| Y656 | Phosphorylation | Uniprot | |
| T661 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| T681 | Phosphorylation | Uniprot | |
| S685 | Phosphorylation | Uniprot | |
| S691 | Phosphorylation | Uniprot | |
| T717 | Phosphorylation | Uniprot | |
| K822 | Ubiquitination | Uniprot | |
| K957 | Ubiquitination | Uniprot | |
| K1033 | Ubiquitination | Uniprot | |
| S1075 | Phosphorylation | Uniprot | |
| K1191 | Methylation | Uniprot |
研究背景
Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.
Phosphorylated; does not inhibit catalytic activity.
Cytoplasm. Cell membrane.
Note: Translocated from the cytoplasm to endosomes in response to stress stimuli (PubMed:12810723). Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association (PubMed:12810723). Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS (PubMed:19710016).
Isoform 2 is ubiquitously expressed. Isoform 1 is expressed only in testis, kidney and colon.
Isoform 1 forms homooligomers through the SAM domain. Isoform 1 is also able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization of isoform 1 inhibits its catalytic activity. Interacts with RAF1 and BRAF.
Belongs to the eukaryotic diacylglycerol kinase family.
研究领域
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Metabolism > Lipid metabolism > Glycerolipid metabolism.
· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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