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| 产品: | MARK4 抗体 |
| 货号: | AF0693 |
| 描述: | Rabbit polyclonal antibody to MARK4 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Dog |
| 分子量: | 82kDa; 83kD(Calculated). |
| 蛋白号: | Q96L34 |
| RRID: | AB_2834302 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0693, RRID:AB_2834302.
展开/折叠
FLJ90097; KIAA1860; MAP / microtubule affinity regulating kinase 4; MAP / microtubule affinity regulating kinase 4L; MAP/microtubule affinity regulating kinase 4; MAP/microtubule affinity regulating kinase like 1; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase-like 1; MARK 4; MARK 4L; MARK L1; MARK4; MARK4 serine / threonine protein kinase; MARK4 serine/threonine protein kinase; MARK4_HUMAN; MARK4S; MARKL 1; MARKL1; Nbla00650; Putative protein product of Nbla00650;
抗原和靶标
Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).
- Q96L34 MARK4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSRTVLAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRNSIASCPEEQPHVGNYRLLRTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLVMEYASAGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLLDAEANIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDIWSLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFLVLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGMGYTREEIKESLTSQKYNEVTATYLLLGRKTEEGGDRGAPGLALARVRAPSDTTNGTSSSKGTSHSKGQRSSSSTYHRQRRHSDFCGPSPAPLHPKRSPTSTGEAELKEERLPGRKASCSTAGSGSRGLPPSSPMVSSAHNPNKAEIPERRKDSTSTPNNLPPSMMTRRNTYVCTERPGAERPSLLPNGKENSSGTPRVPPASPSSHSLAPPSGERSRLARGSTIRSTFHGGQVRDRRAGGGGGGGVQNGPPASPTLAHEAAPLPAGRPRPTTNLFTKLTSKLTRRVADEPERIGGPEVTSCHLPWDQTETAPRLLRFPWSVKLTSSRPPEALMAALRQATAAARCRCRQPQPFLLACLHGGAGGPEPLSHFEVEVCQLPRPGLRGVLFRRVAGTALAFRTLVTRISNDLEL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96L34 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S15 | Phosphorylation | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| T20 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| R60 | Methylation | Uniprot | |
| T64 | Phosphorylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| T214 | Phosphorylation | Q15831 (STK11) | Uniprot |
| S218 | Phosphorylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| S404 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| T440 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| T442 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S496 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| T507 | Phosphorylation | Uniprot | |
| T511 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| R561 | Methylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| T564 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot | |
| T568 | Phosphorylation | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| K622 | Ubiquitination | Uniprot |
研究背景
Serine/threonine-protein kinase. Phosphorylates the microtubule-associated protein MAPT/TAU. Also phosphorylates the microtubule-associated proteins MAP2 and MAP4. Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles. Required for the initiation of axoneme extension during cilium assembly. Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro. Plays a role in cell cycle progression, specifically in the G1/S checkpoint. Reduces neuronal cell survival. Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation.
Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylated throughout the cell cycle.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm>Cytoskeleton>Cilium axoneme. Cytoplasm. Cell projection>Dendrite.
Note: Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.
Ubiquitous. Isoform 2 is brain-specific. Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen.
Interacts with MAPT/TAU. Interacts with gamma-tubulin. Interacts with ODF2. Interacts with USP9X. Interacts with YWHAQ.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
文献引用
Application: IHC Species: Human Sample: gingival tissues
Application: WB Species: Human Sample: gingival tissues
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