IRX2 Antibody - #AF0552

Fig. 1 IRX2 expression was increased in fibrotic hearts. A Relative mRNA levels of 6 Irx family members in adult mouse cardiac fibroblasts (CFs) after angiotensin II (Ang II) treatment for 24 h (n = 5). B Relative mRNA levels of 6 IRX family members in human CFs after Ang II treatment for 24 h (n = 5). C Irx2 mRNA levels in the heart after Ang II infusion by an osmotic minipump (n = 6). D Representative western blots and statistical analysis of IRX2 expression in the heart after Ang II infusion for 12 weeks (n = 6). E Representative western blots and statistical analysis of IRX2 expression in heart samples obtained from patients with dilated cardiomyopathy (DCM) and control (Con) donors (Con, n = 6; DCM, n = 7). F Irx2 mRNA levels in endothelial cells (ECs), cardiomyocytes (CMs) and CFs isolated from hearts infused with Ang II for 12 weeks (n = 6). G Representative western blots and quantification of IRX2 protein expression in CFs isolated from hearts infused with Ang II for 12 weeks (n = 6). Data are shown as the mean ± SEM, and analysed using an unpaired two-tailed Student′s t test. For the analysis in (C), one-way ANOVA with Tamhane’s T2 test was conducted. Source data are provided as a Source Data file.

Fig. 2 Conditional fibroblast-specific Irx2-deficient mice exhibited attenuated fibrotic remodelling in response to angiotensin II (Ang II) infusion. A Conditional fibroblast-specific Irx2-deficient mice (Irx2 cfKO) were bred by crossing mice with a conditional knockout allele of Irx2 (Irx2fl/fl) with Col1α2-Cre mice. Irx2 cfKO mice and littermate controls were subjected to Ang II infusion for 12 weeks. B Representative western blots and statistical analysis of IRX2 protein expression in CFs isolated from Irx2 cfKO mice and littermate controls (n = 5). C–E Irx2 cfKO mice exhibited an attenuated heart weight-to-tibia length (HW/TL) ratio (n = 10 mice, Col1α2-Cre+Saline; n = 12 mice, Irx2fl/fl+Saline; n = 12 mice, Irx2 cfKO+Saline; n = 12 mice, Col1α2-Cre+Ang II; n = 11 mice, Irx2fl/fl+Ang II; n = 12 mice, Irx2 cfKO+Ang II) (C), a reduced fibrosis area (n = 5) (D), and a decreased cell area of cardiomyocytes (n = 5) (E) after Ang II infusion. F Relative mRNA levels of Col1 and Col3 in hearts from Irx2 cfKO mice and littermate controls after Ang II infusion (n = 6). G Cardiac function (n = 6) was improved in Ang II-infused Irx2 cfKO mice. Data are shown as the mean ± SEM, and analysed using one-way ANOVA followed by Tukey post hoc test (E and G) or Tamhane’s T2 test (C, D and F). For the analysis in (B), an unpaired two-tailed Student′s t test was conducted. Source data are provided as a Source Data file.

Fig. 2 Conditional fibroblast-specific Irx2-deficient mice exhibited attenuated fibrotic remodelling in response to angiotensin II (Ang II) infusion. A Conditional fibroblast-specific Irx2-deficient mice (Irx2 cfKO) were bred by crossing mice with a conditional knockout allele of Irx2 (Irx2fl/fl) with Col1α2-Cre mice. Irx2 cfKO mice and littermate controls were subjected to Ang II infusion for 12 weeks. B Representative western blots and statistical analysis of IRX2 protein expression in CFs isolated from Irx2 cfKO mice and littermate controls (n = 5). C–E Irx2 cfKO mice exhibited an attenuated heart weight-to-tibia length (HW/TL) ratio (n = 10 mice, Col1α2-Cre+Saline; n = 12 mice, Irx2fl/fl+Saline; n = 12 mice, Irx2 cfKO+Saline; n = 12 mice, Col1α2-Cre+Ang II; n = 11 mice, Irx2fl/fl+Ang II; n = 12 mice, Irx2 cfKO+Ang II) (C), a reduced fibrosis area (n = 5) (D), and a decreased cell area of cardiomyocytes (n = 5) (E) after Ang II infusion. F Relative mRNA levels of Col1 and Col3 in hearts from Irx2 cfKO mice and littermate controls after Ang II infusion (n = 6). G Cardiac function (n = 6) was improved in Ang II-infused Irx2 cfKO mice. Data are shown as the mean ± SEM, and analysed using one-way ANOVA followed by Tukey post hoc test (E and G) or Tamhane’s T2 test (C, D and F). For the analysis in (B), an unpaired two-tailed Student′s t test was conducted. Source data are provided as a Source Data file.