XRN2 Antibody - #AF0490

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产品: XRN2 抗体
货号: AF0490
描述: Rabbit polyclonal antibody to XRN2
应用: WB IHC IF/ICC
反应: Human, Mouse, Monkey
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 108kDa; 109kD(Calculated).
蛋白号: Q9H0D6
RRID: AB_2834320

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MS Report
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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Monkey
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
XRN2 Antibody detects endogenous levels of total XRN2.
RRID:
AB_2834320
引用格式: Affinity Biosciences Cat# AF0490, RRID:AB_2834320.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5' 3' exoribonuclease 2; DHM1 like protein; DHP protein; XRN 2;

抗原和靶标

免疫原:
Uniprot:

Q9H0D6(XRN2_HUMAN) >>Visit HPA database.

基因/基因ID:
XRN2(22803)
表达:
Q9H0D6 XRN2_HUMAN:

Expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Isoform 2 is expressed predominantly in peripheral blood leukocytes.

描述:
This gene shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The yeast gene is involved in homologous recombination and RNA metabolism, such as RNA synthesis and RNA trafficking. Complementation studies show that Dhm1 has a similar function in mouse as dhp1. The function of the human gene has not yet been determined. Transcript variants encoding different isoforms have been noted for this gene; however, their full-length nature is not known.
序列:
MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIHPCTHPEDKPAPKNEDEMMVAIFEYIDRLFSIVRPRRLLYMAIDGVAPRAKMNQQRSRRFRASKEGMEAAVEKQRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIADRLNNDPGWKNLTVILSDASAPGEGEHKIMDYIRRQRAQPNHDPNTHHCLCGADADLIMLGLATHEPNFTIIREEFKPNKPKPCGLCNQFGHEVKDCEGLPREKKGKHDELADSLPCAEGEFIFLRLNVLREYLERELTMASLPFTFDVERSIDDWVFMCFFVGNDFLPHLPSLEIRENAIDRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDSIFKKRKDDEDSFRRRQKEKRKRMKRDQPAFTPSGILTPHALGSRNSPGSQVASNPRQAAYEMRMQNNSSPSISPNTSFTSDGSPSPLGGIKRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQSYVEGLCWVLRYYYQGCASWKWYYPFHYAPFASDFEGIADMPSDFEKGTKPFKPLEQLMGVFPAASGNFLPPSWRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRAALEEVYPDLTPEETRRNSLGGDVLFVGKHHPLHDFILELYQTGSTEPVEVPPELCHGIQGKFSLDEEAILPDQIVCSPVPMLRDLTQNTVVSINFKDPQFAEDYIFKAVMLPGARKPAAVLKPSDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVMPRGSGTGIYSNAAPPPVTYQGNLYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQQRFDRGVGAEPLLPWNRMLQTQNAAFQPNQYQMLAGPGGYPPRRDDRGGRQGYPREGRKYPLPPPSGRYNWN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9H0D6 作为底物

Site PTM Type Enzyme
K40 Ubiquitination
Y99 Phosphorylation
R120 Methylation
K123 Ubiquitination
K132 Sumoylation
K132 Ubiquitination
K142 Ubiquitination
K152 Ubiquitination
K172 Ubiquitination
Y176 Phosphorylation
Y177 Phosphorylation
K189 Ubiquitination
K207 Ubiquitination
K286 Ubiquitination
K368 Ubiquitination
K373 Ubiquitination
T374 Phosphorylation
T379 Phosphorylation
T433 Phosphorylation
S435 Phosphorylation
T439 Phosphorylation P50750 (CDK9) , P50613 (CDK7) , P28482 (MAPK1)
S445 Phosphorylation
S448 Phosphorylation
S451 Phosphorylation
S455 Phosphorylation
R458 Methylation
Y462 Phosphorylation
S470 Phosphorylation
S471 Phosphorylation
S473 Phosphorylation
S475 Phosphorylation
T478 Phosphorylation
S479 Phosphorylation
T481 Phosphorylation
S485 Phosphorylation
S487 Phosphorylation
K493 Ubiquitination
S499 Phosphorylation
S501 Phosphorylation
K517 Ubiquitination
K524 Methylation
K524 Ubiquitination
K533 Ubiquitination
K537 Ubiquitination
K616 Ubiquitination
K640 Acetylation
K640 Ubiquitination
K641 Ubiquitination
Y666 Phosphorylation
S678 Phosphorylation
K756 Ubiquitination
Y764 Phosphorylation
K767 Ubiquitination
K776 Ubiquitination
K782 Ubiquitination
S789 Phosphorylation
S790 Phosphorylation
K796 Acetylation
K796 Ubiquitination
R806 Methylation
R816 Methylation
R824 Methylation
R847 Methylation
R851 Methylation
K858 Methylation
S861 Phosphorylation
R864 Methylation
R870 Methylation
R880 Methylation
R883 Methylation
R895 Methylation
R921 Methylation
R922 Methylation
R925 Methylation
R928 Methylation
Y931 Phosphorylation
R933 Methylation
R946 Methylation

研究背景

功能:

Possesses 5'->3' exoribonuclease activity (By similarity). May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Binds to RNA polymerase II (RNAp II) transcription termination R-loops formed by G-rich pause sites.

细胞定位:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Isoform 2 is expressed predominantly in peripheral blood leukocytes.

亚基结构:

Interacts with POLR2A and SMN1/SMN2. Interacts with CDKN2AIP and NKRF. Interacts with CDKN2AIPNL; the interaction is direct. Interacts with TRIM71 (via NHL repeats) in an RNA-dependent manner.

蛋白家族:

Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.

研究领域

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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