Phospho-LRP6 (Ser1490) Antibody - #AF8344

Fig. 5. BA promoted β-catenin phosphorylation and inhibited the combination of LRP6 and Wnt3a. Mlg and PPF cells were maintained in the presence of BA (5, 10 and 20 μM) for 2 h and then treated with Wnt3a (100 ng/ml) for another 2 h. (A-B) The expression of pS33/37/T41-β-catenin, pT41/S45-β-catenin and β-catenin in (A) Mlg and (B) PPF cells were evaluated using immunoblot assay. Densitometric analysis of p-β-catenin using β-catenin as the internal reference showed beside the blots and "Fold change" refers to the ratio of the gray analysis value of p-β-catenin to the gray analysis value of β-catenin multiplied by 100%. (C-D) Immunoblot analysis of DVL2, p-LRP6, total LRP6 and GAPDH in (C) Mlg and (D) PPF cells of different group. The immunoblots of p-LRP6 were quantified by densitometry using LRP6 as the internal reference. (E) Mlg cells were treated with BA (10 μM) for 2 h and then added Wnt3a (100 ng/ml) for additional 1 h. The protein levels of Wnt3a and LRP6 were detected by western blot. Results are shown as mean ± SEM, n = 3.

Figure 8.| H2S donor ameliorates bleomycin-induced LRP-6/β-catenin signaling in lung tissue. Protein levels of phosphorylated LRP-6 (p-LRP), LRP-6, phosphorylated GSK-3β (Ser9, p-GSK-3β), GSK-3β and β-catenin in lung tissues were determined in Control, BLM, H2S and BLM + H2S groups. Representative protein bands were presented on the top of the histograms. Data are expressed as mean ± SEM (n=7). * p<0.05, † p<0.01.