NT5C3 Antibody - #AF0491

(3)  
产品: NT5C3 抗体
货号: AF0491
描述: Rabbit polyclonal antibody to NT5C3
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Horse, Sheep, Chicken
分子量: 38kDa; 38kD(Calculated).
蛋白号: Q9H0P0
RRID: AB_2834334

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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(90%), Horse(100%), Sheep(100%), Chicken(80%)
克隆:
Polyclonal
特异性:
NT5C3 Antibody detects endogenous levels of total NT5C3.
RRID:
AB_2834334
引用格式: Affinity Biosciences Cat# AF0491, RRID:AB_2834334.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5NT3A_HUMAN; cN III; cN-III; cNIII; Cytosolic 5' nucleotidase 3; Cytosolic 5' nucleotidase III; Cytosolic 5''-nucleotidase 3; Cytosolic 5''-nucleotidase 3A; Cytosolic 5''-nucleotidase III; Nt5c3a; p36; P5''N-1; P5'N 1; P5'N1; P5N 1; P5N-1; P5N1; PN I; PN-I; PNI; PSN1; Pyrimidine 5' nucleotidase 1; Pyrimidine 5''-nucleotidase 1; UMPH; UMPH1; Uridine 5' monophosphate hydrolase 1; Uridine 5''-monophosphate hydrolase 1;

抗原和靶标

免疫原:
Uniprot:

Q9H0P0(5NT3A_HUMAN) >>Visit HPA database.

基因/基因ID:
NT5C3A(51251)
表达:
Q9H0P0 5NT3A_HUMAN:

Isoforms 1, 3 and 4 are expressed in reticulocytes. Isoform 4 is hardly detectable in bone marrow and fetal liver.

描述:
Pyrimidine 5-prime-nucleotidase (P5N; EC 3.1.3.5), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2; MIM 191720). The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology.
序列:
MRAPSMDRAAVARVGAVASASVCALVAGVVLAQYIFTLKRKTGRKTKIIEMMPEFQKSSVRIKNPTRVEEIICGLIKGGAAKLQIITDFDMTLSRFSYKGKRCPTCHNIIDNCKLVTDECRKKLLQLKEKYYAIEVDPVLTVEEKYPYMVEWYTKSHGLLVQQALPKAKLKEIVAESDVMLKEGYENFFDKLQQHSIPVFIFSAGIGDVLEEVIRQAGVYHPNVKVVSNFMDFDETGVLKGFKGELIHVFNKHDGALRNTEYFNQLKDNSNIILLGDSQGDLRMADGVANVEHILKIGYLNDRVDELLEKYMDSYDIVLVQDESLEVANSILQKIL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Sheep
100
Pig
90
Chicken
80
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9H0P0 作为底物

Site PTM Type Enzyme
K47 Ubiquitination
K57 Ubiquitination
K77 Ubiquitination
K128 Ubiquitination
K130 Ubiquitination
K167 Ubiquitination
K225 Ubiquitination
S228 Phosphorylation
T236 Phosphorylation
K243 Ubiquitination
K252 Ubiquitination
T260 Phosphorylation
K267 Ubiquitination
S278 Phosphorylation
K296 Ubiquitination
R303 Methylation
K334 Ubiquitination

研究背景

功能:

Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP). CMP seems to be the preferred substrate.

细胞定位:

Cytoplasm.

Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoforms 1, 3 and 4 are expressed in reticulocytes. Isoform 4 is hardly detectable in bone marrow and fetal liver.

亚基结构:

Monomer.

蛋白家族:

Belongs to the pyrimidine 5'-nucleotidase family.

研究领域

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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