Phospho-TRPV1(Ser502) Antibody - #AF8520

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产品: 磷酸化 TRPV1(Ser502) 抗体
货号: AF8520
描述: Rabbit polyclonal antibody to Phospho-TRPV1(Ser502)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q8NER1
RRID: AB_2840574

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-VR1 (Ser502) Antibody detects endogenous levels of VR1 only when phosphorylated at Ser502.
RRID:
AB_2840574
引用格式: Affinity Biosciences Cat# AF8520, RRID:AB_2840574.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Capsaicin receptor; DKFZp434K0220; osm 9 like TRP channel 1; Osm-9-like TRP channel 1; OTRPC1; Transient receptor potential cation channel subfamily V member 1; TRPV 1; Trpv1; TRPV1_HUMAN; Vanilloid receptor 1; Vanilloid receptor subtype 1; VR 1; VR1;

抗原和靶标

免疫原:

A synthesized peptide derived from human VR1 around the phosphorylation site of Ser502.

基因/基因ID:
TRPV1(7442)

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

文献引用

1). Selective activation of AKAP150/TRPV1 in ventrolateral periaqueductal gray GABAergic neurons facilitates conditioned place aversion in male mice. Communications Biology, 2023 (PubMed: 37460788) [IF=5.9]

Application: WB    Species: Mouse    Sample:

Fig. 5 TRPV1 in GABAergic neurons of the vlPAG in CPA mice. Expression of TRPV1 in the pinch pain CPA model (a) and morphine withdrawal CPA model (b). (two sample t test, n = 6). p-TRPV1 was upregulated in the pinch pain CPA model (c, d) and morphine withdrawal CPA model (e, f) [immunofluorescence image (c, e), scale bar, 50 μm (n = 3); western blotting (d, f), ***P 

Application: IF/ICC    Species: Mouse    Sample:

Fig. 5 TRPV1 in GABAergic neurons of the vlPAG in CPA mice. Expression of TRPV1 in the pinch pain CPA model (a) and morphine withdrawal CPA model (b). (two sample t test, n = 6). p-TRPV1 was upregulated in the pinch pain CPA model (c, d) and morphine withdrawal CPA model (e, f) [immunofluorescence image (c, e), scale bar, 50 μm (n = 3); western blotting (d, f), ***P 
2). Pingchong Jiangni recipe through nerve growth factor/transient receptor potential vanilloid 1 signaling pathway to relieve pain in endometriosis model rats. Journal of Ethnopharmacology, 2024 (PubMed: 37479067) [IF=4.8]
3). Esketamine alleviates hypoxia/reoxygenation injury of cardiomyocytes by regulating TRPV1 expression and inhibiting intracellular Ca2+ concentration. Clinics (Sao Paulo, Brazil), 2024 (PubMed: 38692008) [IF=2.2]
4). Activation of Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII) with Lidocaine Provokes Pyroptosis of Glioblastoma Cells. BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2021 (PubMed: 34302204) [IF=0.9]

Application: WB    Species: Human    Sample: U87-MG cells

Fig. 1. LA activated TRPV1 through CaMKII to induce Ca 2+ overload. a) CaMKII content in U87-MG cells (Western blotting): 1) negative control (NC); 2) CaMKII-silencing (CaMKIIA); b) phosphorylation of TRPV1 in U87-MG cells (Western blotting): 1) NC; 2) NC+lidocaine; 3) CaMKIIA+lidocaine. c) Effect of different concentrations of lidocaine on intracellular Ca 2+ concentration in control U87-MG cells and cells transfected with si-CaMKII (CaMKIIA) (flow cytometry). p<0.05 in comparison with *NC, + CaMKIIA+lidocaine.

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