| 产品: | UBE2E3 抗体 |
| 货号: | DF10000 |
| 描述: | Rabbit polyclonal antibody to UBE2E3 |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 23 kDa; 23kD(Calculated). |
| 蛋白号: | Q969T4 |
| RRID: | AB_2840580 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF10000, RRID:AB_2840580.
展开/折叠
Homologous to yeast UBC4/5; UB2E3_HUMAN; UBC E4; Ubc M2; UBC4/5 homolog; UBCE 4; UBCE4; UBCH 9; UbcH9; UbcM2; UBE2 E3; UBE2E3; Ubiquitin carrier protein; Ubiquitin carrier protein E3; Ubiquitin conjugating enzyme E2 23 kDa; Ubiquitin conjugating enzyme E2 E3; Ubiquitin conjugating enzyme E2E 3 (homologous to yeast UBC4/5); Ubiquitin conjugating enzyme E2E 3 (UBC4/5 homolog, yeast; Ubiquitin conjugating enzyme E2E 3; Ubiquitin conjugating enzyme UBCH 9; Ubiquitin conjugating enzyme UBCH9; Ubiquitin protein ligase; Ubiquitin protein ligase E3; Ubiquitin-conjugating enzyme E2 E3; Ubiquitin-conjugating enzyme E2-23 kDa; Ubiquitin-protein ligase E3;
抗原和靶标
- Q969T4 UB2E3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSDRQRSDDESPSTSSGSSDADQRDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q969T4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| Y91 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot |
研究背景
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.
Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner.
Ubiquitously expressed at low levels. Highly expressed in skeletal muscle.
The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus. Interacts with NEDD4L.
Belongs to the ubiquitin-conjugating enzyme family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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