| 产品: | ATP6V0A2 抗体 |
| 货号: | DF10006 |
| 描述: | Rabbit polyclonal antibody to ATP6V0A2 |
| 应用: | WB IHC |
| 反应: | Human |
| 预测: | Rat, Bovine, Horse, Sheep, Dog |
| 分子量: | 98 kDa; 98kD(Calculated). |
| 蛋白号: | Q9Y487 |
| RRID: | AB_2840586 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF10006, RRID:AB_2840586.
展开/折叠
a2; A2V ATPase; ARCL; ATP6a2; ATP6N1D; ATP6V0A2; ATPase, H+ transporting, lysosomal V0 subunit a isoform 2; ATPase, H+ transporting, lysosomal V0 subunit a2; Infantile malignant osteopetrosis; J6B7; Lysosomal H(+) transporting ATPase V0 subunit a2; Lysosomal H(+)-transporting ATPase V0 subunit a2; regeneration and tolerance factor; Stv1; TJ6; TJ6M; TJ6s; V ATPase 116 kDa isoform a2; V type proton ATPase 116 kDa subunit a; V type proton ATPase 116 kDa subunit a isoform 2; V-ATPase 116 kDa isoform a2; V-type proton ATPase 116 kDa subunit a isoform 2; Vacuolar proton translocating ATPase 116 kDa subunit a; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2; Vph1; VPP2_HUMAN; WSS;
抗原和靶标
- Q9Y487 VPP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGSLFRSETMCLAQLFLQSGTAYECLSALGEKGLVQFRDLNQNVSSFQRKFVGEVKRCEELERILVYLVQEINRADIPLPEGEASPPAPPLKQVLEMQEQLQKLEVELREVTKNKEKLRKNLLELIEYTHMLRVTKTFVKRNVEFEPTYEEFPSLESDSLLDYSCMQRLGAKLGFVSGLINQGKVEAFEKMLWRVCKGYTIVSYAELDESLEDPETGEVIKWYVFLISFWGEQIGHKVKKICDCYHCHVYPYPNTAEERREIQEGLNTRIQDLYTVLHKTEDYLRQVLCKAAESVYSRVIQVKKMKAIYHMLNMCSFDVTNKCLIAEVWCPEADLQDLRRALEEGSRESGATIPSFMNIIPTKETPPTRIRTNKFTEGFQNIVDAYGVGSYREVNPALFTIITFPFLFAVMFGDFGHGFVMFLFALLLVLNENHPRLNQSQEIMRMFFNGRYILLLMGLFSVYTGLIYNDCFSKSVNLFGSGWNVSAMYSSSHPPAEHKKMVLWNDSVVRHNSILQLDPSIPGVFRGPYPLGIDPIWNLATNRLTFLNSFKMKMSVILGIIHMTFGVILGIFNHLHFRKKFNIYLVSIPELLFMLCIFGYLIFMIFYKWLVFSAETSRVAPSILIEFINMFLFPASKTSGLYTGQEYVQRVLLVVTALSVPVLFLGKPLFLLWLHNGRSCFGVNRSGYTLIRKDSEEEVSLLGSQDIEEGNHQVEDGCREMACEEFNFGEILMTQVIHSIEYCLGCISNTASYLRLWALSLAHAQLSDVLWAMLMRVGLRVDTTYGVLLLLPVIALFAVLTIFILLIMEGLSAFLHAIRLHWVEFQNKFYVGAGTKFVPFSFSLLSSKFNNDDSVA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y487 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K92 | Ubiquitination | Uniprot | |
| K115 | Acetylation | Uniprot | |
| Y128 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| Y149 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| Y163 | Phosphorylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| K279 | Acetylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| S491 | Phosphorylation | Uniprot | |
| S492 | Phosphorylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| Y688 | Phosphorylation | Uniprot | |
| K693 | Ubiquitination | Uniprot | |
| S695 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | Uniprot |
研究背景
Part of the proton channel of V-ATPases. Essential component of the endosomal pH-sensing machinery. May play a role in maintaining the Golgi functions, such as glycosylation maturation, by controlling the Golgi pH. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation.
Cell membrane>Multi-pass membrane protein. Endosome membrane.
Note: In kidney proximal tubules, also detected in subapical vesicles.
The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''. Directly interacts with PSCD2 through its N-terminal cytosolic tail in an intra-endosomal acidification-dependent manner. Disruption of this interaction results in the inhibition of endocytosis. Interacts with SPAAR.
Belongs to the V-ATPase 116 kDa subunit family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Immune diseases > Rheumatoid arthritis.
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
· Organismal Systems > Excretory system > Collecting duct acid secretion.
限制条款
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