VCAN Antibody - #DF10007

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产品: VCAN 抗体
货号: DF10007
描述: Rabbit polyclonal antibody to VCAN
应用: WB IHC
反应: Human
预测: Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 373 kDa; 373kD(Calculated).
蛋白号: P13611
RRID: AB_2840587

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Bovine(91%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
VCAN Antibody detects endogenous levels of total VCAN.
RRID:
AB_2840587
引用格式: Affinity Biosciences Cat# DF10007, RRID:AB_2840587.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Chondroitin sulfate proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; Chondroitin sulfate proteoglycan core protein, cartilage; CSPG2; CSPG2_HUMAN; ERVR; GHAP; Glial hyaluronate binding protein; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; PG-M; PGM; VCAN; Versican; Versican core protein; Versican proteoglycan; WGN 1; WGN; WGN1;

抗原和靶标

免疫原:
Uniprot:

P13611(CSPG2_HUMAN) >>Visit HPA database.

基因/基因ID:
VCAN(1462)
表达:
P13611 CSPG2_HUMAN:

Expressed in the retina (at protein level) (PubMed:29777959). Cerebral white matter and plasma (PubMed:2469524). Isoform V0: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas (PubMed:8627343). Isoform V1: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas (PubMed:8627343). Isoform V2: Restricted to normal brain and gliomas (PubMed:8627343). Isoform V3: Found in all these tissues except medulloblastomas (PubMed:8627343).

序列:
MFINIKSILWMCSTLIVTHALHKVKVGKSPPVRGSLSGKVSLPCHFSTMPTLPPSYNTSEFLRIKWSKIEVDKNGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEAVGDASLTVVKLLASDAGLYRCDVMYGIEDTQDTVSLTVDGVVFHYRAATSRYTLNFEAAQKACLDVGAVIATPEQLFAAYEDGFEQCDAGWLADQTVRYPIRAPRVGCYGDKMGKAGVRTYGFRSPQETYDVYCYVDHLDGDVFHLTVPSKFTFEEAAKECENQDARLATVGELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKPKEATTIDLSILAETASPSLSKEPQMVSDRTTPIIPLVDELPVIPTEFPPVGNIVSFEQKATVQPQAITDSLATKLPTPTGSTKKPWDMDDYSPSASGPLGKLDISEIKEEVLQSTTGVSHYATDSWDGVVEDKQTQESVTQIEQIEVGPLVTSMEILKHIPSKEFPVTETPLVTARMILESKTEKKMVSTVSELVTTGHYGFTLGEEDDEDRTLTVGSDESTLIFDQIPEVITVSKTSEDTIHTHLEDLESVSASTTVSPLIMPDNNGSSMDDWEERQTSGRITEEFLGKYLSTTPFPSQHRTEIELFPYSGDKILVEGISTVIYPSLQTEMTHRRERTETLIPEMRTDTYTDEIQEEITKSPFMGKTEEEVFSGMKLSTSLSEPIHVTESSVEMTKSFDFPTLITKLSAEPTEVRDMEEDFTATPGTTKYDENITTVLLAHGTLSVEAATVSKWSWDEDNTTSKPLESTEPSASSKLPPALLTTVGMNGKDKDIPSFTEDGADEFTLIPDSTQKQLEEVTDEDIAAHGKFTIRFQPTTSTGIAEKSTLRDSTTEEKVPPITSTEGQVYATMEGSALGEVEDVDLSKPVSTVPQFAHTSEVEGLAFVSYSSTQEPTTYVDSSHTIPLSVIPKTDWGVLVPSVPSEDEVLGEPSQDILVIDQTRLEATISPETMRTTKITEGTTQEEFPWKEQTAEKPVPALSSTAWTPKEAVTPLDEQEGDGSAYTVSEDELLTGSERVPVLETTPVGKIDHSVSYPPGAVTEHKVKTDEVVTLTPRIGPKVSLSPGPEQKYETEGSSTTGFTSSLSPFSTHITQLMEETTTEKTSLEDIDLGSGLFEKPKATELIEFSTIKVTVPSDITTAFSSVDRLHTTSAFKPSSAITKKPPLIDREPGEETTSDMVIIGESTSHVPPTTLEDIVAKETETDIDREYFTTSSPPATQPTRPPTVEDKEAFGPQALSTPQPPASTKFHPDINVYIIEVRENKTGRMSDLSVIGHPIDSESKEDEPCSEETDPVHDLMAEILPEFPDIIEIDLYHSEENEEEEEECANATDVTTTPSVQYINGKHLVTTVPKDPEAAEARRGQFESVAPSQNFSDSSESDTHPFVIAKTELSTAVQPNESTETTESLEVTWKPETYPETSEHFSGGEPDVFPTVPFHEEFESGTAKKGAESVTERDTEVGHQAHEHTEPVSLFPEESSGEIAIDQESQKIAFARATEVTFGEEVEKSTSVTYTPTIVPSSASAYVSEEEAVTLIGNPWPDDLLSTKESWVEATPRQVVELSGSSSIPITEGSGEAEEDEDTMFTMVTDLSQRNTTDTLITLDTSRIITESFFEVPATTIYPVSEQPSAKVVPTKFVSETDTSEWISSTTVEEKKRKEEEGTTGTASTFEVYSSTQRSDQLILPFELESPNVATSSDSGTRKSFMSLTTPTQSEREMTDSTPVFTETNTLENLGAQTTEHSSIHQPGVQEGLTTLPRSPASVFMEQGSGEAAADPETTTVSSFSLNVEYAIQAEKEVAGTLSPHVETTFSTEPTGLVLSTVMDRVVAENITQTSREIVISERLGEPNYGAEIRGFSTGFPLEEDFSGDFREYSTVSHPIAKEETVMMEGSGDAAFRDTQTSPSTVPTSVHISHISDSEGPSSTMVSTSAFPWEEFTSSAEGSGEQLVTVSSSVVPVLPSAVQKFSGTASSIIDEGLGEVGTVNEIDRRSTILPTAEVEGTKAPVEKEEVKVSGTVSTNFPQTIEPAKLWSRQEVNPVRQEIESETTSEEQIQEEKSFESPQNSPATEQTIFDSQTFTETELKTTDYSVLTTKKTYSDDKEMKEEDTSLVNMSTPDPDANGLESYTTLPEATEKSHFFLATALVTESIPAEHVVTDSPIKKEESTKHFPKGMRPTIQESDTELLFSGLGSGEEVLPTLPTESVNFTEVEQINNTLYPHTSQVESTSSDKIEDFNRMENVAKEVGPLVSQTDIFEGSGSVTSTTLIEILSDTGAEGPTVAPLPFSTDIGHPQNQTVRWAEEIQTSRPQTITEQDSNKNSSTAEINETTTSSTDFLARAYGFEMAKEFVTSAPKPSDLYYEPSGEGSGEVDIVDSFHTSATTQATRQESSTTFVSDGSLEKHPEVPSAKAVTADGFPTVSVMLPLHSEQNKSSPDPTSTLSNTVSYERSTDGSFQDRFREFEDSTLKPNRKKPTENIIIDLDKEDKDLILTITESTILEILPELTSDKNTIIDIDHTKPVYEDILGMQTDIDTEVPSEPHDSNDESNDDSTQVQEIYEAAVNLSLTEETFEGSADVLASYTQATHDESMTYEDRSQLDHMGFHFTTGIPAPSTETELDVLLPTATSLPIPRKSATVIPEIEGIKAEAKALDDMFESSTLSDGQAIADQSEIIPTLGQFERTQEEYEDKKHAGPSFQPEFSSGAEEALVDHTPYLSIATTHLMDQSVTEVPDVMEGSNPPYYTDTTLAVSTFAKLSSQTPSSPLTIYSGSEASGHTEIPQPSALPGIDVGSSVMSPQDSFKEIHVNIEATFKPSSEEYLHITEPPSLSPDTKLEPSEDDGKPELLEEMEASPTELIAVEGTEILQDFQNKTDGQVSGEAIKMFPTIKTPEAGTVITTADEIELEGATQWPHSTSASATYGVEAGVVPWLSPQTSERPTLSSSPEINPETQAALIRGQDSTIAASEQQVAARILDSNDQATVNPVEFNTEVATPPFSLLETSNETDFLIGINEESVEGTAIYLPGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDQCELDFDECHSNPCRNGATCVDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
91
Sheep
91
Pig
0
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P13611 作为底物

Site PTM Type Enzyme
K68 Acetylation
K79 Acetylation
K98 Acetylation
K98 Ubiquitination
Y208 Phosphorylation
S364 Phosphorylation
S368 Phosphorylation
S510 O-Glycosylation
T516 O-Glycosylation
T518 O-Glycosylation
T522 O-Glycosylation
T627 O-Glycosylation
T632 O-Glycosylation
S641 O-Glycosylation
T642 O-Glycosylation
T642 Phosphorylation
T643 O-Glycosylation
S647 Phosphorylation
T687 O-Glycosylation
T689 O-Glycosylation
T716 O-Glycosylation
T716 Phosphorylation
S727 Phosphorylation
T751 O-Glycosylation
T754 O-Glycosylation
T792 O-Glycosylation
T799 O-Glycosylation
S801 O-Glycosylation
T869 O-Glycosylation
T886 O-Glycosylation
T887 O-Glycosylation
S888 O-Glycosylation
T889 O-Glycosylation
S976 O-Glycosylation
T981 O-Glycosylation
T1092 O-Glycosylation
T1093 O-Glycosylation
S1101 O-Glycosylation
S1103 O-Glycosylation
T1110 O-Glycosylation
T1116 O-Glycosylation
T1121 O-Glycosylation
T1123 O-Glycosylation
S1131 O-Glycosylation
S1133 O-Glycosylation
T1191 O-Glycosylation
S1212 O-Glycosylation
T1219 O-Glycosylation
T1220 O-Glycosylation
S1221 O-Glycosylation
S1349 Phosphorylation
S1351 Phosphorylation
T1418 O-Glycosylation
T1419 O-Glycosylation
T1566 O-Glycosylation
T1569 O-Glycosylation
S1716 O-Glycosylation
T1719 O-Glycosylation
T1731 O-Glycosylation
T1734 O-Glycosylation
S1742 O-Glycosylation
S1743 O-Glycosylation
T1744 O-Glycosylation
T1763 O-Glycosylation
S1765 O-Glycosylation
T1769 O-Glycosylation
S1772 O-Glycosylation
S1775 O-Glycosylation
T1777 O-Glycosylation
T1778 O-Glycosylation
T1780 O-Glycosylation
S1909 O-Glycosylation
S1925 O-Glycosylation
T1926 O-Glycosylation
S1935 O-Glycosylation
S1942 O-Glycosylation
T1943 O-Glycosylation
S1945 O-Glycosylation
T2036 O-Glycosylation
T2050 Phosphorylation
S2058 O-Glycosylation
S2058 Phosphorylation
T2059 O-Glycosylation
T2059 Phosphorylation
T2063 O-Glycosylation
T2069 O-Glycosylation
T2083 O-Glycosylation
S2085 O-Glycosylation
T2086 O-Glycosylation
T2086 Phosphorylation
T2091 O-Glycosylation
S2099 Phosphorylation
T2115 Phosphorylation
S2116 Phosphorylation
T2152 O-Glycosylation
T2153 O-Glycosylation
T2159 O-Glycosylation
T2160 O-Glycosylation
T2163 Phosphorylation
T2194 O-Glycosylation
T2200 O-Glycosylation
S2203 O-Glycosylation
K2238 Methylation
T2331 Phosphorylation
S2337 Phosphorylation
T2416 O-Glycosylation
S2417 O-Glycosylation
S2422 O-Glycosylation
T2448 O-Glycosylation
S2455 O-Glycosylation
S2456 O-Glycosylation
T2457 O-Glycosylation
T2458 O-Glycosylation
S2473 O-Glycosylation
T2503 O-Glycosylation
T2505 O-Glycosylation
T2509 O-Glycosylation
S2515 O-Glycosylation
T2516 O-Glycosylation
S2530 O-Glycosylation
T2531 O-Glycosylation
T2540 O-Glycosylation
T2617 Phosphorylation
T2656 O-Glycosylation
S2661 O-Glycosylation
S2699 O-Glycosylation
T2701 O-Glycosylation
S2781 O-Glycosylation
T2784 O-Glycosylation
T2785 O-Glycosylation
S2941 Phosphorylation
T2950 O-Glycosylation
S3024 O-Glycosylation
T3025 O-Glycosylation
S3029 O-Glycosylation
T3205 Phosphorylation
S3206 Phosphorylation
S3209 Phosphorylation
K3312 Acetylation
S3363 Phosphorylation

研究背景

功能:

May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

翻译修饰:

Phosphorylated by FAM20C in the extracellular medium.

细胞定位:

Secreted>Extracellular space>Extracellular matrix. Cell projection>Cilium>Photoreceptor outer segment. Secreted>Extracellular space>Extracellular matrix>Interphotoreceptor matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the retina (at protein level). Cerebral white matter and plasma. Isoform V0: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V1: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2: Restricted to normal brain and gliomas. Isoform V3: Found in all these tissues except medulloblastomas.

亚基结构:

Interacts with FBLN1.

蛋白家族:

Belongs to the aggrecan/versican proteoglycan family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

文献引用

1). Establishment and functional characterization of immortalized rabbit dermal papilla cell lines. Animal biotechnology, 2023 (PubMed: 37652434) [IF=3.7]

Application: IF/ICC    Species: rabbit    Sample: DPCs and R-7 cell

Figure 2. Indirect immunofluorescence staining of primary DPCs and R-7. All the DPCs were incubated with antibodies against α-SMA, SV40LT and Versican (Scale Bar: 50 µm). Passage 5 of DPCs were used.
2). miR-543 impairs cell proliferation, migration, and invasion in breast cancer by suppressing VCAN. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 34293593) [IF=3.1]

Application: WB    Species: Human    Sample: BC cell

Fig. 4. The low expression of VCAN blocked BC cell malignancy caused by miR-543 inhibitor. (A) VCAN protein levels in MDA-MB-231 and MCF-7 treated with si-VCAN þ miR- 543 inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC were detected by western blotting. (B) Cell viability of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543 inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC were detected by CCK-8 assay. (C) Cell proliferation of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543 inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC were detected by BrdU assay. (D) Cell migration of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543 inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC was detected by wound healing assay. (E) Cell invasion of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543 inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC was detected by transwell assay. *P < 0.05, **P < 0.001 versus negative control group. #P < 0.05, ##P < 0.001 versus si- VCAN þ miR-543 inhibitor. Data are presented as the mean ± SD.
3). Exosomes Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation through the Wnt3a/β-Catenin Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 36388171)

Application: IF/ICC    Species: Mouse    Sample:

Figure 1 Morphology and indirect immunofluorescence staining of HFSCs and DPCs. (a) α-SMA and Versican antibodies were incubated with DPCs (passage 2) (scale bar: 50 μm). (b) Alkaline phosphatase staining was examined with DPCs (passage 8) (scale bar: 100 μm, 50 μm). (c) KRT14, KRT15, and KRT19 antibodies were incubated with HFSCs (passage 2) (scale bar: 50 μm).

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