产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0430, RRID:AB_2834339.
展开/折叠
2310016K04Rik; dihydrouridine synthase 2 like, SMM1 homolog (S. cerevisiae); DUS2; FLJ20399; SMM1; tRNA dihydrouridine synthase 2 like; Up regulated in lung cancer; URLC8;
抗原和靶标
Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).
- Q9NX74 DUS2L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MILNSLSLCYHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDDRVVFRTCEREQNRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKQYSTKGGMGAALLSDPDKIEKILSTLVKGTRRPVTCKIRILPSLEDTLSLVKRIERTGIAAIAVHGRKREERPQHPVSCEVIKAIADTLSIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQLESPQGRLLHAAQSSREICEAFGLGAFYEETTQELDAQQARLSAKTSEQTGEPAEDTSGVIKMAVKFDRRAYPAQITPKMCLLEWCRREKLAQPVYETVQRPLDRLFSSIVTVAEQKYQSTLWDKSKKLAEQAAAIVCLRSQGLPEGRLGEESPSLHKRKREAPDQDPGGPRAQELAQPGDLCKKPFVALGSGEESPLEGW
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NX74 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | Uniprot | |
| Y10 | Phosphorylation | Uniprot | |
| T24 | Phosphorylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| T234 | Phosphorylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| Y283 | Phosphorylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot |
研究背景
Dihydrouridine synthase. Catalyzes the NADPH-dependent synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR.
Cytoplasm. Endoplasmic reticulum.
Note: Mainly at the endoplasmic reticulum.
Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).
Interacts with EPRS1. Interacts (via DRBM domain) with PRKRA and EIF2AK2/PKR (via DRBM 1 domain).
Efficient dihydrouridine synthesis requires the presence of both the catalytic domain and the C-terminal RNA-binding DRBM domain.
Belongs to the Dus family. Dus2 subfamily.
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