产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF10058, RRID:AB_2840638.
展开/折叠
2310075E07Rik; AW546441; Cavin 1; Cavin; Cavin-1; CAVIN1; CGL4; FKSG13; FLJ90031; MGC118550; OTTMUSP00000002049; Polymerase I and transcript release factor; Ptrf; PTRF_HUMAN; RNA polymerase I and transcript release factor; RP23-279L23.6; TTF I interacting peptide 12;
抗原和靶标
- Q6NZI2 CAVN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDPTLYIVERPLPGYPDAEAPEPSSAGAQAAEEPSGAGSEELIKSDQVNGVLVLSLLDKIIGAVDQIQLTQAQLEERQAEMEGAVQSIQGELSKLGKAHATTSNTVSKLLEKVRKVSVNVKTVRGSLERQAGQIKKLEVNEAELLRRRNFKVMIYQDEVKLPAKLSISKSLKESEALPEKEGEELGEGERPEEDAAALELSSDEAVEVEEVIEESRAERIKRSGLRRVDDFKKAFSKEKMEKTKVRTRENLEKTRLKTKENLEKTRHTLEKRMNKLGTRLVPAERREKLKTSRDKLRKSFTPDHVVYARSKTAVYKVPPFTFHVKKIREGQVEVLKATEMVEVGADDDEGGAERGEAGDLRRGSSPDVHALLEITEESDAVLVDKSDSD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q6NZI2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| T103 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K109 | Acetylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| K116 | Sumoylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K152 | Acetylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| K161 | Acetylation | Uniprot | |
| K161 | Sumoylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K165 | Acetylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| K170 | Sumoylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| K173 | Sumoylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| T269 | Phosphorylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| S300 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| T302 | Phosphorylation | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| T313 | Phosphorylation | Uniprot | |
| Y316 | Phosphorylation | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| K326 | Acetylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| K327 | Acetylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| T376 | Phosphorylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot |
研究背景
Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues. Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1). Essential for normal oligomerization of CAV1. Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop. Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template (By similarity). The caveolae biogenesis pathway is required for the secretion of proteins such as GASK1A (By similarity).
Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity. Phosphorylation at Tyr-156 is essential for its functionin the regulation of ribosomal transcriptional activity.
Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.
Monoubiquitinated.
Membrane>Caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm>Cytosol. Mitochondrion. Nucleus.
Note: Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Colocalizes with CAV1 in lipid rafts in adipocytes. Localizes in the caveolae in a caveolin-dependent manner (By similarity).
Component of the CAVIN complex composed of CAVIN1, CAVIN2, CAVIN3 and CAVIN4. Homotrimer (By similarity). Interacts with TTF1. Interacts with RNA polymerase I. Binds the 3' end of pre-rRNA. Interacts with transcription factor ZNF148 (By similarity). Interacts with LIPE in the adipocyte cytoplasm. Interacts with CAV1 and CAVIN3 (By similarity). Interacts with CAVIN2. Interacts with CAVIN4 and CAV3.
The leucine-zipper domain 1 is essential for its localization in the caveolae.
Belongs to the CAVIN family.
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