产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF10081, RRID:AB_2840661.
展开/折叠
Gar1; GAR1_HUMAN; H/ACA ribonucleoprotein complex subunit 1; nucleolar protein family A member 1 (H/ACA small nucleolar RNPs); Nucleolar protein family A member 1; snoRNP protein GAR1;
抗原和靶标
- Q9NY12 GAR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSFRGGGRGGFNRGGGGGGFNRGGSSNHFRGGGGGGGGGNFRGGGRGGFGRGGGRGGFNKGQDQGPPERVVLLGEFLHPCEDDIVCKCTTDENKVPYFNAPVYLENKEQIGKVDEIFGQLRDFYFSVKLSENMKASSFKKLQKFYIDPYKLLPLQRFLPRPPGEKGPPRGGGRGGRGGGRGGGGRGGGRGGGFRGGRGGGGGGFRGGRGGGFRGRGH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NY12 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R8 | Methylation | Uniprot | |
| R13 | Methylation | Uniprot | |
| R22 | Methylation | Uniprot | |
| R30 | Methylation | Uniprot | |
| R51 | Methylation | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| Y97 | Phosphorylation | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| K134 | Sumoylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| Y149 | Phosphorylation | Uniprot | |
| K150 | Acetylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| R185 | Methylation | Uniprot | |
| R189 | Methylation | Uniprot | |
| R194 | Methylation | Uniprot | |
| R197 | Methylation | Uniprot | |
| R205 | Methylation | Uniprot | |
| R208 | Methylation | Uniprot |
研究背景
Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Nucleus>Nucleolus. Nucleus>Cajal body.
Note: Also localized to Cajal bodies (coiled bodies).
Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which contains NHP2/NOLA2, GAR1/NOLA1, NOP10/NOLA3, and DKC1/NOLA4, which is presumed to be the catalytic subunit. The complex contains a stable core formed by binding of one or two NOP10-DKC1 heterodimers to NHP2; GAR1 subsequently binds to this core via DKC1. The complex binds a box H/ACA small nucleolar RNA (snoRNA), which may target the specific site of modification within the RNA substrate. The complex also interacts with TERC, which contains a 3'-terminal domain related to the box H/ACA snoRNAs. Specific interactions with snoRNAs or TERC are mediated by GAR1 and NHP2. Associates with NOLC1/NOPP140. H/ACA snoRNPs interact with the SMN complex, consisting of SMN1 or SMN2, GEMIN2/SIP1, DDX20/GEMIN3, and GEMIN4. This is mediated by interaction between GAR1 and SMN1 or SMN2. The SMN complex may be required for correct assembly of the H/ACA snoRNP complex. Component of the telomerase holoenzyme complex composed of one molecule of TERT, one molecule of WRAP53/TCAB1, two molecules of H/ACA ribonucleoprotein complex subunits DKC1, NOP10, NHP2 and GAR1, and a telomerase RNA template component (TERC). The telomerase holoenzyme complex is associated with TEP1, SMG6/EST1A and POT1.
Interaction with SMN1 requires at least one of the RGG-box regions.
Belongs to the GAR1 family.
研究领域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
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