| 产品: | SETMAR 抗体 |
| 货号: | DF10118 |
| 描述: | Rabbit polyclonal antibody to SETMAR |
| 应用: | WB |
| 反应: | Human |
| 预测: | Bovine, Dog |
| 分子量: | 78 kDa; 78kD(Calculated). |
| 蛋白号: | Q53H47 |
| RRID: | AB_2840698 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF10118, RRID:AB_2840698.
展开/折叠
Histone lysine N methyltransferase; Histone lysine N methyltransferase SETMAR; Hsmar 1; Hsmar1; Mariner transposase Hsmar1; Metnase; SET domain and mariner transposase fusion; SET domain and mariner transposase fusion gene; SET domain and mariner transposase fusion gene containing protein; SET domain and mariner transposase fusion gene-containing protein; Setmar; SETMR_HUMAN;
抗原和靶标
Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle.
- Q53H47 SETMR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFAEAAKTTRPCGMAEFKEKPEAPTEQLDVACGQENLPVGAWPPGAAPAPFQYTPDHVVGPGADIDPTQITFPGCICVKTPCLPGTCSCLRHGENYDDNSCLRDIGSGGKYAEPVFECNVLCRCSDHCRNRVVQKGLQFHFQVFKTHKKGWGLRTLEFIPKGRFVCEYAGEVLGFSEVQRRIHLQTKSDSNYIIAIREHVYNGQVMETFVDPTYIGNIGRFLNHSCEPNLLMIPVRIDSMVPKLALFAAKDIVPEEELSYDYSGRYLNLTVSEDKERLDHGKLRKPCYCGAKSCTAFLPFDSSLYCPVEKSNISCGNEKEPSMCGSAPSVFPSCKRLTLETMKMMLDKKQIRAIFLFEFKMGRKAAETTRNINNAFGPGTANERTVQWWFKKFCKGDESLEDEERSGRPSEVDNDQLRAIIEADPLTTTREVAEELNVNHSTVVRHLKQIGKVKKLDKWVPHELTENQKNRRFEVSSSLILRNHNEPFLDRIVTCDEKWILYDNRRRSAQWLDQEEAPKHFPKPILHPKKVMVTIWWSAAGLIHYSFLNPGETITSEKYAQEIDEMNQKLQRLQLALVNRKGPILLHDNARPHVAQPTLQKLNELGYEVLPHPPYSPDLLPTNYHVFKHLNNFLQGKRFHNQQDAENAFQEFVESQSTDFYATGINQLISRWQKCVDCNGSYFD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q53H47 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K275 | Ubiquitination | Uniprot | |
| K335 | Methylation | Uniprot | |
| T338 | Phosphorylation | Uniprot | |
| T341 | Phosphorylation | Uniprot | |
| K343 | Acetylation | Uniprot | |
| K348 | Acetylation | Uniprot | |
| K391 | Acetylation | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| K448 | Acetylation | Uniprot | |
| K498 | Methylation | Uniprot | |
| S508 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| K523 | Ubiquitination | Uniprot | |
| K569 | Ubiquitination | Uniprot | |
| K637 | Ubiquitination | Uniprot | |
| K674 | Ubiquitination | Uniprot |
研究背景
Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Has also kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity. In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining. Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A.
Methylated. Methylation regulates activity in DNA decatenation.
Phosphorylated at Ser-508 by CHEK1 and dephosphorylated by protein phosphatase 2A/PP2A. Phosphorylation at Ser-508 is enhanced by DNA damage and promotes recruitment to damaged DNA. It stimulates DNA repair and impairs replication fork restart.
Nucleus. Chromosome.
Note: Recruited on damaged DNA at sites of double-strand breaks.
Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle.
Homodimer. Interacts with PRPF19; required for SETMAR recruitment to damaged DNA sites. Interacts with PCNA. Interacts with TOP2A; stimulates TOP2A topoisomerase activity. May interact with RAD9A and/or RAD9B.
The mariner transposase Hsmar1 region mediates DNA-binding. It has retained some of the nucleases activity but has lost its transposase activity because the active site contains an Asn in position 610 instead of an Asp residue.
In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.
In the N-terminal section; belongs to the class V-like SAM-binding methyltransferase superfamily.
In the C-terminal section; belongs to the mariner transposase family.
研究领域
· Metabolism > Amino acid metabolism > Lysine degradation.
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