产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0176, RRID:AB_2833369.
展开/折叠
75 kDa glucose regulated protein; 75 kDa glucose-regulated protein; CSA; Glucose Regulated Protein; Grp 75; GRP-75; GRP75; GRP75_HUMAN; Heat shock 70 kDa protein 9; Heat shock 70kD protein 9; heat shock 70kDa protein 9; Heat shock 70kDa protein 9B; Heat shock protein 74 kDa A; Heat shock protein A; Heat shock protein cognate 74; Hsc74; Hsp74; Hsp74a; HSPA9; Hspa9a; HSPA9B; MGC4500; mitochondrial; Mortalin 2; Mortalin; Mortalin perinuclear; Mortalin2; MOT 2; MOT; MOT2; Mthsp70; p66 mortalin; P66 MOT; PBP74; Peptide binding protein 74; Peptide-binding protein 74; Stress 70 protein mitochondrial; Stress 70 protein mitochondrial precursor; Stress-70 protein;
抗原和靶标
- P38646 GRP75_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MISASRAAAARLVGAAASRGPTAARHQDSWNGLSHEAFRLVSRRDYASEAIKGAVVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGEDFDQALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P38646 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| T22 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| Y46 | Phosphorylation | Uniprot | |
| T62 | Phosphorylation | P33981 (TTK) | Uniprot |
| S65 | Phosphorylation | P33981 (TTK) | Uniprot |
| K76 | Ubiquitination | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| T87 | Phosphorylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| T111 | Phosphorylation | Uniprot | |
| Y118 | Phosphorylation | Uniprot | |
| T120 | Phosphorylation | Uniprot | |
| K121 | Acetylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| Y128 | Phosphorylation | Uniprot | |
| K135 | Acetylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K138 | Acetylation | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| K143 | Acetylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K159 | Acetylation | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| Y181 | Phosphorylation | Uniprot | |
| T185 | Phosphorylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| Y196 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| T205 | Phosphorylation | Uniprot | |
| K206 | Acetylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| R218 | Methylation | Uniprot | |
| Y230 | Phosphorylation | Uniprot | |
| K234 | Acetylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| K288 | Acetylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K291 | Acetylation | Uniprot | |
| K300 | Acetylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| R307 | Methylation | Uniprot | |
| T333 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K345 | Acetylation | Uniprot | |
| T356 | Phosphorylation | Uniprot | |
| R360 | Methylation | Uniprot | |
| T362 | Phosphorylation | Uniprot | |
| K368 | Acetylation | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| K377 | Acetylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| K394 | Acetylation | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| T398 | Phosphorylation | Uniprot | |
| R405 | Methylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| T462 | Phosphorylation | Uniprot | |
| T480 | Phosphorylation | Uniprot | |
| C487 | S-Nitrosylation | Uniprot | |
| K498 | Acetylation | Uniprot | |
| T504 | Phosphorylation | Uniprot | |
| R513 | Methylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| K567 | Acetylation | Uniprot | |
| Y568 | Phosphorylation | Uniprot | |
| K595 | Acetylation | Uniprot | |
| K600 | Acetylation | Uniprot | |
| C608 | S-Nitrosylation | Uniprot | |
| K610 | Acetylation | Uniprot | |
| K612 | Acetylation | Uniprot | |
| K612 | Ubiquitination | Uniprot | |
| S616 | Phosphorylation | Uniprot | |
| R624 | Methylation | Uniprot | |
| S627 | Phosphorylation | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| S639 | Phosphorylation | Uniprot | |
| K646 | Acetylation | Uniprot | |
| K646 | Ubiquitination | Uniprot | |
| Y652 | Phosphorylation | Uniprot | |
| K653 | Methylation | Uniprot | |
| K653 | Ubiquitination | Uniprot | |
| S662 | Phosphorylation | Uniprot | |
| K671 | Acetylation | Uniprot | |
| K675 | Acetylation | Uniprot | |
| K678 | Acetylation | Uniprot |
研究背景
Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU. Regulates erythropoiesis via stabilization of ISC assembly. May play a role in the control of cell proliferation and cellular aging (By similarity).
Mitochondrion. Nucleus>Nucleolus.
Interacts strongly with the intermediate form of FXN and weakly with its mature form. Interacts with HSCB. Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1, MTX2 and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. Interacts with DNLZ, the interaction is required to prevent self-aggregation. Interacts with TESPA1. Interacts with PDPN. Interacts with NFU1, NFS1 and ISCU.
Belongs to the heat shock protein 70 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
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