LTBP1 Antibody - #DF10191

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产品: LTBP1 抗体
货号: DF10191
描述: Rabbit polyclonal antibody to LTBP1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 187kDa; 187kD(Calculated).
蛋白号: Q14766
RRID: AB_2840770

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(89%), Bovine(89%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
LTBP1 Antibody detects endogenous levels of total LTBP1.
RRID:
AB_2840770
引用格式: Affinity Biosciences Cat# DF10191, RRID:AB_2840770.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

9430031G15Rik; 9830146M04; Latent transforming growth factor beta binding protein 1; Latent-transforming growth factor beta-binding protein 1; Latent-transforming growth factor beta-binding protein, isoform 1L; Latent-transforming growth factor beta-binding protein, isoform 1S; LBT1S; LTBP-1; Ltbp1; LTBP1_HUMAN; MGC163161; TGF-beta1-BP-1; Tgfb; Transforming growth factor beta-1-binding protein 1; Transforming growth factor beta-1-masking protein large subunit;

抗原和靶标

免疫原:
Uniprot:

Q14766(LTBP1_HUMAN) >>Visit HPA database.

基因/基因ID:
LTBP1(4052)
表达:
Q14766 LTBP1_HUMAN:

Expressed in the aorta (at protein level) (PubMed:17293099). Isoform Long: Expressed in fibroblasts (PubMed:17293099).

序列:
MAGAWLRWGLLLWAGLLASSAHGRLRRITYVVHPGPGLAAGALPLSGPPRSRTFNVALNARYSRSSAAAGAPSRASPGVPSERTRRTSKPGGAALQGLRPPPPPPPEPARPAVPGGQLHPNPGGHPAAAPFTKQGRQVVRSKVPQETQSGGGSRLQVHQKQQLQGVNVCGGRCCHGWSKAPGSQRCTKPSCVPPCQNGGMCLRPQLCVCKPGTKGKACETIAAQDTSSPVFGGQSPGAASSWGPPEQAAKHTSSKKADTLPRVSPVAQMTLTLKPKPSVGLPQQIHSQVTPLSSQSVVIHHGQTQEYVLKPKYFPAQKGISGEQSTEGSFPLRYVQDQVAAPFQLSNHTGRIKVVFTPSICKVTCTKGSCQNSCEKGNTTTLISENGHAADTLTATNFRVVICHLPCMNGGQCSSRDKCQCPPNFTGKLCQIPVHGASVPKLYQHSQQPGKALGTHVIHSTHTLPLTVTSQQGVKVKFPPNIVNIHVKHPPEASVQIHQVSRIDGPTGQKTKEAQPGQSQVSYQGLPVQKTQTIHSTYSHQQVIPHVYPVAAKTQLGRCFQETIGSQCGKALPGLSKQEDCCGTVGTSWGFNKCQKCPKKPSYHGYNQMMECLPGYKRVNNTFCQDINECQLQGVCPNGECLNTMGSYRCTCKIGFGPDPTFSSCVPDPPVISEEKGPCYRLVSSGRQCMHPLSVHLTKQLCCCSVGKAWGPHCEKCPLPGTAAFKEICPGGMGYTVSGVHRRRPIHHHVGKGPVFVKPKNTQPVAKSTHPPPLPAKEEPVEALTFSREHGPGVAEPEVATAPPEKEIPSLDQEKTKLEPGQPQLSPGISTIHLHPQFPVVIEKTSPPVPVEVAPEASTSSASQVIAPTQVTEINECTVNPDICGAGHCINLPVRYTCICYEGYRFSEQQRKCVDIDECTQVQHLCSQGRCENTEGSFLCICPAGFMASEEGTNCIDVDECLRPDVCGEGHCVNTVGAFRCEYCDSGYRMTQRGRCEDIDECLNPSTCPDEQCVNSPGSYQCVPCTEGFRGWNGQCLDVDECLEPNVCANGDCSNLEGSYMCSCHKGYTRTPDHKHCRDIDECQQGNLCVNGQCKNTEGSFRCTCGQGYQLSAAKDQCEDIDECQHRHLCAHGQCRNTEGSFQCVCDQGYRASGLGDHCEDINECLEDKSVCQRGDCINTAGSYDCTCPDGFQLDDNKTCQDINECEHPGLCGPQGECLNTEGSFHCVCQQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGEAFCENVEGSFLCVCADENQEYSPMTGQCRSRTSTDLDVDVDQPKEEKKECYYNLNDASLCDNVLAPNVTKQECCCTSGVGWGDNCEIFPCPVLGTAEFTEMCPKGKGFVPAGESSSEAGGENYKDADECLLFGQEICKNGFCLNTRPGYECYCKQGTYYDPVKLQCFDMDECQDPSSCIDGQCVNTEGSYNCFCTHPMVLDASEKRCIRPAESNEQIEETDVYQDLCWEHLSDEYVCSRPLVGKQTTYTECCCLYGEAWGMQCALCPLKDSDDYAQLCNIPVTGRRQPYGRDALVDFSEQYTPEADPYFIQDRFLNSFEELQAEECGILNGCENGRCVRVQEGYTCDCFDGYHLDTAKMTCVDVNECDELNNRMSLCKNAKCINTDGSYKCLCLPGYVPSDKPNYCTPLNTALNLEKDSDLE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Chicken
100
Rabbit
100
Pig
89
Bovine
89
Sheep
89
Xenopus
78
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14766 作为底物

Site PTM Type Enzyme
S66 Phosphorylation
S296 O-Glycosylation
T304 O-Glycosylation
K318 Ubiquitination
S321 Phosphorylation
S325 Phosphorylation
T326 O-Glycosylation
T357 O-Glycosylation
S438 O-Glycosylation
S460 O-Glycosylation
T461 O-Glycosylation
T463 O-Glycosylation
T467 O-Glycosylation
T469 O-Glycosylation
S470 O-Glycosylation
S501 O-Glycosylation
Y523 Phosphorylation
T531 Phosphorylation
T533 O-Glycosylation
S536 O-Glycosylation
T537 O-Glycosylation
S539 O-Glycosylation
S738 Phosphorylation
S768 O-Glycosylation
T769 O-Glycosylation
T785 O-Glycosylation
T801 O-Glycosylation
S810 O-Glycosylation
Y896 Phosphorylation
T897 Phosphorylation
Y901 Phosphorylation
Y904 Phosphorylation
Y988 Phosphorylation
T991 Phosphorylation
T1333 O-Glycosylation
S1413 Phosphorylation
S1414 Phosphorylation
S1415 Phosphorylation
Y1422 Phosphorylation
S1597 Phosphorylation
Y1600 Phosphorylation
T1601 Phosphorylation
S1616 Phosphorylation
Y1688 Phosphorylation

研究背景

功能:

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Outcompeted by LRRC32/GARP for binding to LAP regulatory chain of TGF-beta.

翻译修饰:

Contains hydroxylated asparagine residues.

Isoform Short N-terminus is blocked.

Two intrachain disulfide bonds from the TB3 domain are rearranged upon TGFB1 binding, and form interchain bonds with TGFB1 propeptide, anchoring it to the extracellular matrix.

细胞定位:

Secreted. Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the aorta (at protein level). Isoform Long: Expressed in fibroblasts.

亚基结构:

Interacts with TGFB1; associates via disulfide bonds with the Latency-associated peptide chain (LAP) regulatory chain of TGFB1, leading to regulate activation of TGF-beta-1. LTBP1 does not bind directly to TGF-beta-1, the active chain of TGFB1. Interacts (via C-terminal domain) with FBN1 (via N-terminal domain). Interacts with FBN2. Interacts with ADAMTSL2.

蛋白家族:

The 8-Cys3 region in the third TB domain mediates the interchain disulfide bond interaction with the Latency-associated peptide chain (LAP) regulatory chain of TGFB1.

Belongs to the LTBP family.

研究领域

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

文献引用

1). Slow Interstitial Fluid Flow Activates TGF-β Signaling and Drives Fibrotic Responses in Human Tenon Fibroblasts. Cells, 2023 (PubMed: 37681937) [IF=6.0]

Application: IHC    Species: Human    Sample:

Figure 6 F-actin and LTBP1 under static and flow conditions in the 2D model. Effect of continuous flow (150 µL/h, 72 h) on LTBP1 in the 2D model. Image acquisition and representation settings are identical for all conditions. The figure is representative of three independent experiments.

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