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产品:	C3AR1 抗体
货号:	DF10198
描述:	Rabbit polyclonal antibody to C3AR1
应用:	WB
反应:	Human
预测:	Rabbit
分子量:	54 kDa; 54kD(Calculated).
蛋白号:	Q16581
RRID:	AB_2840777

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二抗
封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human

预测:

Rabbit(83%)

克隆:

Polyclonal

特异性:

C3AR1 Antibody detects endogenous levels of total C3AR1.

RRID:

AB_2840777
引用格式: Affinity Biosciences Cat# DF10198, RRID:AB_2840777.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

AZ 3B; AZ3B; C3a anaphylatoxin chemotactic receptor; C3A R; C3A R1; C3a-R; C3AR; C3AR_HUMAN; C3AR1; C3R1; Complement component 3 receptor 1; Complement component 3a receptor 1; HNFAG09;

抗原和靶标

免疫原:

Uniprot:

Q16581(C3AR_HUMAN) >>Visit HPA database.

基因/基因ID:

C3AR1(719)

表达:

Q16581 C3AR_HUMAN:

Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.

序列:

Q16581 C3AR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASFSAETNSTDLLSQPWNEPPVILSMVILSLTFLLGLPGNGLVLWVAGLKMQRTVNTIWFLHLTLADLLCCLSLPFSLAHLALQGQWPYGRFLCKLIPSIIVLNMFASVFLLTAISLDRCLVVFKPIWCQNHRNVGMACSICGCIWVVAFVMCIPVFVYREIFTTDNHNRCGYKFGLSSSLDYPDFYGDPLENRSLENIVQPPGEMNDRLDPSSFQTNDHPWTVPTVFQPQTFQRPSADSLPRGSARLTSQNLYSNVFKPADVVSPKIPSGFPIEDHETSPLDNSDAFLSTHLKLFPSASSNSFYESELPQGFQDYYNLGQFTDDDQVPTPLVAITITRLVVGFLLPSVIMIACYSFIVFRMQRGRFAKSQSKTFRVAVVVVAVFLVCWTPYHIFGVLSLLTDPETPLGKTLMSWDHVCIALASANSCFNPFLYALLGKDFRKKARQSIQGILEAAFSEELTRSTHCPSNNVISERNSTTV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Rabbit

Bovine

Horse

Sheep

Dog

Chicken

Pig

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q16581 作为底物

Q16581

Site	PTM Type	Source
S266	O-Glycosylation	Uniprot
S266	Phosphorylation	Uniprot
S349	Phosphorylation	Uniprot
S459	Phosphorylation	Uniprot
T463	Phosphorylation	Uniprot
S465	Phosphorylation	Uniprot
T466	Phosphorylation	Uniprot
S470	Phosphorylation	Uniprot
S475	Phosphorylation	Uniprot
S479	Phosphorylation	Uniprot
T480	Phosphorylation	Uniprot
T481	Phosphorylation	Uniprot

研究背景

Q16581_C3AR_HUMAN

功能:

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

翻译修饰:

Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.

O-glycosylated.

细胞定位:

Cell membrane>Multi-pass membrane protein.

组织特异性:

亚基结构:

Interacts with VGF-derived peptide TLQP-21 (By similarity).

蛋白家族:

Belongs to the G-protein coupled receptor 1 family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

文献引用

1). Aristolochic acid I promotes the invasion and migration of hepatocellular carcinoma cells by activating the C3a/C3aR complement system. TOXICOLOGY LETTERS, 2020 (PubMed: 32898628) [IF=3.5]

Application: WB Species: mouse Sample: Hepa1-6 cells

Fig 4.| The eff ects of AAI exposure on C3a secretion and C3aR mRNA and protein expression in Hepa1-6 cells. Hepa1-6 cells were exposed to diff erent concentrations of AAI (0, 2.5, 5, or 10 μM) for 24 h. (A) Enzyme-linked immunosorbent assay was performed to measure AAI-induced excess C3a secretion (B) Western blot assays were performed to measure the expression of the C3aR protein.

Application: IF/ICC Species: mouse Sample: Hepa1-6 cells

Fig 4.| The eff ects of AAI exposure on C3a secretion and C3aR mRNA and protein expression in Hepa1-6 cells. Hepa1-6 cells were exposed to diff erent concentrations of AAI (0, 2.5, 5, or 10 μM) for 24 h. (D) Immunofluorescence images stained for C3aR (green) and DAPI (blue) Scale bar, 50 μm. Each bar represents the mean ±SD based on three replicates. P < 0.05 was considered to be statistically significant. ** compared with vehicle control (0 μM), P <0.01; ***compared with vehicle control (0 μM), P < 0.001; ****compared with vehicle control(0 μM), P < 0.0001

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C3AR1 Antibody - #DF10198

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