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产品:	CALCRL 抗体
货号:	DF10203
描述:	Rabbit polyclonal antibody to CALCRL
应用:	WB
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量:	53 kDa; 53kD(Calculated).
蛋白号:	Q16602
RRID:	AB_2840782

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

联系销售

实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(86%)

克隆:

Polyclonal

特异性:

CALCRL Antibody detects endogenous levels of total CALCRL.

RRID:

AB_2840782
引用格式: Affinity Biosciences Cat# DF10203, RRID:AB_2840782.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Calcitonin gene related peptide type 1 receptor; Calcitonin receptor like; Calcitonin receptor like receptor; CALCRL; CGRP type 1 receptor; CGRPR; CRLR;

抗原和靶标

免疫原:

Uniprot:

Q16602(CALRL_HUMAN) >>Visit HPA database.

基因/基因ID:

CALCRL(10203)

表达:

Q16602 CALRL_HUMAN:

Predominantly expressed in the lung and heart.

序列:

Q16602 CALRL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEKKCTLNFLVLLPFFMILVTAELEESPEDSIQLGVTRNKIMTAQYECYQKIMQDPIQQAEGVYCNRTWDGWLCWNDVAAGTESMQLCPDYFQDFDPSEKVTKICDQDGNWFRHPASNRTWTNYTQCNVNTHEKVKTALNLFYLTIIGHGLSIASLLISLGIFFYFKSLSCQRITLHKNLFFSFVCNSVVTIIHLTAVANNQALVATNPVSCKVSQFIHLYLMGCNYFWMLCEGIYLHTLIVVAVFAEKQHLMWYYFLGWGFPLIPACIHAIARSLYYNDNCWISSDTHLLYIIHGPICAALLVNLFFLLNIVRVLITKLKVTHQAESNLYMKAVRATLILVPLLGIEFVLIPWRPEGKIAEEVYDYIMHILMHFQGLLVSTIFCFFNGEVQAILRRNWNQYKIQFGNSFSNSEALRSASYTVSTISDGPGYSHDCPSEHLNGKSIHDIENVLLKPENLYN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Rabbit

100

Dog

100

Pig

Horse

Bovine

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q16602 作为底物

Q16602

Site	PTM Type	Source
S285	Phosphorylation	Uniprot
K403	Ubiquitination	Uniprot
S409	Phosphorylation	Uniprot
S445	Phosphorylation	Uniprot
Y460	Phosphorylation	Uniprot

研究背景

Q16602_CALRL_HUMAN

功能:

Receptor for calcitonin-gene-related peptide (CGRP) together with RAMP1 and receptor for adrenomedullin together with RAMP3 (By similarity). Receptor for adrenomedullin together with RAMP2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

细胞定位:

Cell membrane>Multi-pass membrane protein.

组织特异性:

Predominantly expressed in the lung and heart.

亚基结构:

Heterodimer of CALCRL and RAMP3 (By similarity). Heterodimer of CALCRL and RAMP1 or CALCRL and RAMP2.

蛋白家族:

Belongs to the G-protein coupled receptor 2 family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

文献引用

1). Intermedin in Paraventricular Nucleus Attenuates Ang II-Induced Sympathoexcitation through the Inhibition of NADPH Oxidase-Dependent ROS Generation in Obese Rats with Hypertension. International Journal of Molecular Sciences, 2019 (PubMed: 31466304) [IF=5.6]

Application: WB Species: rat Sample: paraventricular nucleus

Figure 1. |Plasma norepinephrine (NE) level (A. n = 6 or 7), systolic blood pressure (B. n = 6–7); and relative values of intermedin (IMD), calcitonin receptor–like receptor (CRLR) and receptor activity–modifying protein (RAMP) 1, RAMP2, and RAMP3 protein expression in the paraventricular nucleus (PVN) in control and obesity-related hypertensive (OH) rats (C–H. n = 3–4). Values are mean± SEM. * p < 0.05 versus control.

2). Exogenous CGRP Regulates Apoptosis and Autophagy to Alleviate Traumatic Brain Injury Through Akt/mTOR Signalling Pathway. NEUROCHEMICAL RESEARCH, 2020 (PubMed: 33063244) [IF=4.4]

Application: WB Species: mouse Sample: brain cortical

Fig. 1 CGRP alleviated the brain lesion induced by TBI. a Relative protein expression of CGRP in the brain cortical tissues with or without CGRP administration after TBI over time was detected by western blot (loading volume of CGRP: 15 μg).

3). Adrenomedullin ameliorates palmitic acid-induced insulin resistance through PI3K/Akt pathway in adipocytes. ACTA DIABETOLOGICA, 2022 (PubMed: 34978596) [IF=3.8]

4). Correlation of vascular change with TRPV1, TRPV4, and TRPA1 in a rat model of inferior gluteal artery perforator flap. WOUND REPAIR AND REGENERATION, 2022 (PubMed: 35384152) [IF=2.9]

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CALCRL Antibody - #DF10203

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