Collagen XIV alpha 1 Antibody - #AF0573

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产品: Collagen XIV alpha 1 抗体
货号: AF0573
描述: Rabbit polyclonal antibody to Collagen XIV alpha 1
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Bovine, Rabbit, Dog, Xenopus
分子量: 193kDa; 194kD(Calculated).
蛋白号: Q05707
RRID: AB_2834370

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MS Report
HPLC Report
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COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Bovine(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Collagen XIV alpha 1 Antibody detects endogenous levels of total Collagen XIV alpha 1.
RRID:
AB_2834370
引用格式: Affinity Biosciences Cat# AF0573, RRID:AB_2834370.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Collagen alpha 1(XIV) chain; Collagen type XIV alpha 1 (undulin); Collagen type XIV alpha 1; UND; Undulin (fibronectin tenascin related); Undulin; COL14A1;

抗原和靶标

免疫原:
Uniprot:

Q05707(COEA1_HUMAN) >>Visit HPA database.

基因/基因ID:
COL14A1(7373)
描述:
COL14A1 Plays an adhesive role by integrating collagen bundles. It is probably associated with the surface of interstitial collagen fibrils via COL1. The COL2 domain may then serve as a rigid arm which sticks out from the fibril and protrudes the large N-terminal globular domain into the extracellular space, where it might interact with other matrix molecules or cell surface receptors. Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
序列:
MKIFQRKMRYWLLPPFLAIVYFCTIVQGQVAPPTRLRYNVISHDSIQISWKAPRGKFGGYKLLVTPTSGGKTNQLNLQNTATKAIIQGLMPDQNYTVQIIAYNKDKESKPAQGQFRIKDLEKRKDPKPRVKVVDRGNGSRPSSPEEVKFVCQTPAIADIVILVDGSWSIGRFNFRLVRHFLENLVTAFDVGSEKTRIGLAQYSGDPRIEWHLNAFSTKDEVIEAVRNLPYKGGNTLTGLALNYIFENSFKPEAGSRTGVSKIGILITDGKSQDDIIPPSRNLRESGVELFAIGVKNADVNELQEIASEPDSTHVYNVAEFDLMHTVVESLTRTLCSRVEEQDREIKASAHAITGPPTELITSEVTARSFMVNWTHAPGNVEKYRVVYYPTRGGKPDEVVVDGTVSSTVLKNLMSLTEYQIAVFAIYAHTASEGLRGTETTLALPMASDLLLYDVTENSMRVKWDAVPGASGYLILYAPLTEGLAGDEKEMKIGETHTDIELSGLLPNTEYTVTVYAMFGEEASDPVTGQETTLALSPPRNLRISNVGSNSARLTWDPTSRQINGYRIVYNNADGTEINEVEVDPITTFPLKGLTPLTEYTIAIFSIYDEGQSEPLTGVFTTEEVPAQQYLEIDEVTTDSFRVTWHPLSADEGLHKLMWIPVYGGKTEEVVLKEEQDSHVIEGLEPGTEYEVSLLAVLDDGSESEVVTAVGTTLDSFWTEPATTIVPTTSVTSVFQTGIRNLVVGDETTSSLRVKWDISDSDVQQFRVTYMTAQGDPEEEVIGTVMVPGSQNNLLLKPLLPDTEYKVTVTPIYTDGEGVSVSAPGKTLPSSGPQNLRVSEEWYNRLRITWDPPSSPVKGYRIVYKPVSVPGPTLETFVGADINTILITNLLSGMDYNVKIFASQASGFSDALTGMVKTLFLGVTNLQAKHVEMTSLCAHWQVHRHATAYRVVIESLQDRQKQESTVGGGTTRHCFYGLQPDSEYKISVYTKLQEIEGPSVSIMEKTQSLPTRPPTFPPTIPPAKEVCKAAKADLVFMVDGSWSIGDENFNKIISFLYSTVGALNKIGTDGTQVAMVQFTDDPRTEFKLNAYKTKETLLDAIKHISYKGGNTKTGKAIKYVRDTLFTAESGTRRGIPKVIVVITDGRSQDDVNKISREMQLDGYSIFAIGVADADYSELVSIGSKPSARHVFFVDDFDAFKKIEDELITFVCETASATCPVVHKDGIDLAGFKMMEMFGLVEKDFSSVEGVSMEPGTFNVFPCYQLHKDALVSQPTRYLHPEGLPSDYTISFLFRILPDTPQEPFALWEILNKNSDPLVGVILDNGGKTLTYFNYDQSGDFQTVTFEGPEIRKIFYGSFHKLHIVVSETLVKVVIDCKQVGEKAMNASANITSDGVEVLGKMVRSRGPGGNSAPFQLQMFDIVCSTSWANTDKCCELPGLRDDESCPDLPHSCSCSETNEVALGPAGPPGGPGLRGPKGQQGEPGPKGPDGPRGEIGLPGPQGPPGPQGPSGLSIQGMPGMPGEKGEKGDTGLPGPQGIPGGVGSPGRDGSPGQRGLPGKDGSSGPPGPPGPIGIPGTPGVPGITGSMGPQGALGPPGVPGAKGERGERGDLQSQAMVRSVARQVCEQLIQSHMARYTAILNQIPSHSSSIRTVQGPPGEPGRPGSPGAPGEQGPPGTPGFPGNAGVPGTPGERGLTGIKGEKGNPGVGTQGPRGPPGPAGPSGESRPGSPGPPGSPGPRGPPGHLGVPGPQGPSGQPGYCDPSSCSAYGVRAPHPDQPEFTPVQDELEAMELWGPGV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
78
Pig
0
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q05707 作为底物

Site PTM Type Enzyme
N94 N-Glycosylation
N372 N-Glycosylation
T439 Phosphorylation
T643 O-Glycosylation
S648 Phosphorylation
Y662 Phosphorylation
S963 Phosphorylation
T970 Phosphorylation
Y988 Phosphorylation
T989 Phosphorylation
T1067 Phosphorylation
T1070 Phosphorylation
T1078 Phosphorylation
Y1118 Phosphorylation
T1122 Phosphorylation
T1125 Phosphorylation
S1128 Phosphorylation
N1384 N-Glycosylation
N1388 N-Glycosylation
S1648 Phosphorylation
S1664 Phosphorylation
S1728 Phosphorylation
S1734 Phosphorylation
Y1758 Phosphorylation
Y1767 Phosphorylation

研究背景

功能:

Plays an adhesive role by integrating collagen bundles. It is probably associated with the surface of interstitial collagen fibrils via COL1. The COL2 domain may then serve as a rigid arm which sticks out from the fibril and protrudes the large N-terminal globular domain into the extracellular space, where it might interact with other matrix molecules or cell surface receptors (By similarity).

翻译修饰:

Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

May contain numerous cysteine residues involved in inter- and intramolecular disulfide bonding.

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homotrimer.

蛋白家族:

Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.

研究领域

· Organismal Systems > Digestive system > Protein digestion and absorption.

文献引用

1). Whole-exome mutational landscape and molecular marker study in mucinous and clear cell ovarian cancer cell lines 3AO and ES2. BMC Cancer, 2023 [IF=3.8]

Application: WB    Species: Human    Sample:

Fig. 6 Differential expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells. (A) Western blotting was used to detect the expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells. In order to improve the clarity and conciseness of the presentation, we cropped the blots. The original, untreated blots images can be found in Additional file 6. (B-D) Statistical analysis of Western blotting results of CCDC170, COL14A1 and THBS2 proteins. (E-G) The expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells was detected by immunofluorescence assay. Original magnification: 40×. (H-J) Statistical analysis of mean fluorescence density of CCDC170, COL14A1 and THBS2 proteins. The gray values of protein bands and the fluorescence intensity of immunofluorescence were analyzed using Image J software (National Institutes of Health, Bethesda, MD, USA).

Application: IF/ICC    Species: Human    Sample:

Fig. 6 Differential expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells. (A) Western blotting was used to detect the expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells. In order to improve the clarity and conciseness of the presentation, we cropped the blots. The original, untreated blots images can be found in Additional file 6. (B-D) Statistical analysis of Western blotting results of CCDC170, COL14A1 and THBS2 proteins. (E-G) The expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells was detected by immunofluorescence assay. Original magnification: 40×. (H-J) Statistical analysis of mean fluorescence density of CCDC170, COL14A1 and THBS2 proteins. The gray values of protein bands and the fluorescence intensity of immunofluorescence were analyzed using Image J software (National Institutes of Health, Bethesda, MD, USA).
2). Identification of Underlying Hub Genes Associated with Hypertrophic Cardiomyopathy by Integrated Bioinformatics Analysis. Pharmacogenomics and Personalized Medicine, 2021 (PubMed: 34285551)

Application: WB    Species: Mouse    Sample: myocardium

Figure 8 Western blotting tests representing protein levels of hub genes (FRZB, COL14A1, CRISPLD1, LUM, sFRP4) and remodeling gene RCAN in myocardium of sham and TAC mice. Abbreviations: FRZB, frizzled related protein; COL14A1, collagen type XIV alpha 1 chain; CRISPLD1, cysteine rich secretory protein LCCL domain containing 1; LUM, lumican; sFRP4, secreted frizzled related protein 4; RCAN, calcipressin regulatory protein.

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