产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2913, RRID:AB_2840902.
展开/折叠
180 kDa bullous pemphigoid antigen 2; Alpha 1 type XVII collagen; BA16H23.2; BP 180; BP180; BPA 2; BPAG 2; BPAG2; Bullous pemphigoid antigen 2; COL17A1; Collagen 17; Collagen alpha 1 XVII chain; Collagen alpha 1(XVII) chain; Collagen alpha1 XVII chain; Collagen type XVII alpha 1; Collagen XVII alpha 1 polypeptide; CollagenXVII; Epidermolysis bullosa junctional localisata variant; FLJ60881; KIAA0204; LAD 1; LAD1;
抗原和靶标
Detected in skin (PubMed:8618013). In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells (at protein level) (PubMed:25676728). Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.
- Q9UMD9 COHA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVTKKNKRDGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTHGSSGYINSTGSTRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTRHAYEGSSSGNSSPEYPRKEFASSSTRGRSQTRESEIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSSNTLPIPKKGTVETKIVTASSQSVSGTYDATILDANLPSHVWSSTLPAGSSMGTYHNNMTTQSSSLLNTNAYSAGSVFGVPNNMASCSPTLHPGLSTSSSVFGMQNNLAPSLTTLSHGTTTTSTAYGVKKNMPQSPAAVNTGVSTSAACTTSVQSDDLLHKDCKFLILEKDNTPAKKEMELLIMTKDSGKVFTASPASIAATSFSEDTLKKEKQAAYNADSGLKAEANGDLKTVSTKGKTTTADIHSYGSSGGGGSGGGGGVGGAGGGPWGPAPAWCPCGSCCSWWKWLLGLLLTWLLLLGLLFGLIALAEEVRKLKARVDELERIRRSILPYGDSMDRIEKDRLQGMAPAAGADLDKIGLHSDSQEELWMFVRKKLMMEQENGNLRGSPGPKGDMGSPGPKGDRGFPGTPGIPGPLGHPGPQGPKGQKGSVGDPGMEGPMGQRGREGPMGPRGEAGPPGSGEKGERGAAGEPGPHGPPGVPGSVGPKGSSGSPGPQGPPGPVGLQGLRGEVGLPGVKGDKGPMGPPGPKGDQGEKGPRGLTGEPGMRGLPGAVGEPGAKGAMGPAGPDGHQGPRGEQGLTGMPGIRGPPGPSGDPGKPGLTGPQGPQGLPGTPGRPGIKGEPGAPGKIVTSEGSSMLTVPGPPGPPGAMGPPGPPGAPGPAGPAGLPGHQEVLNLQGPPGPPGPRGPPGPSIPGPPGPRGPPGEGLPGPPGPPGSFLSNSETFLSGPPGPPGPPGPKGDQGPPGPRGHQGEQGLPGFSTSGSSSFGLNLQGPPGPPGPQGPKGDKGDPGVPGALGIPSGPSEGGSSSTMYVSGPPGPPGPPGPPGSISSSGQEIQQYISEYMQSDSIRSYLSGVQGPPGPPGPPGPVTTITGETFDYSELASHVVSYLRTSGYGVSLFSSSISSEDILAVLQRDDVRQYLRQYLMGPRGPPGPPGASGDGSLLSLDYAELSSRILSYMSSSGISIGLPGPPGPPGLPGTSYEELLSLLRGSEFRGIVGPPGPPGPPGIPGNVWSSISVEDLSSYLHTAGLSFIPGPPGPPGPPGPRGPPGVSGALATYAAENSDSFRSELISYLTSPDVRSFIVGPPGPPGPQGPPGDSRLLSTDASHSRGSSSSSHSSSVRRGSSYSSSMSTGGGGAGSLGAGGAFGEAAGDRGPYGTDIGPGGGYGAAAEGGMYAGNGGLLGADFAGDLDYNELAVRVSESMQRQGLLQGMAYTVQGPPGQPGPQGPPGISKVFSAYSNVTADLMDFFQTYGAIQGPPGQKGEMGTPGPKGDRGPAGPPGHPGPPGPRGHKGEKGDKGDQVYAGRRRRRSIAVKP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UMD9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y40 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| Y80 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| T105 | Phosphorylation | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| T153 | Phosphorylation | Uniprot | |
| T156 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| Y305 | Phosphorylation | Uniprot | |
| K365 | Acetylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K369 | Acetylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| Y396 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| Y512 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S544 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S640 | Phosphorylation | Uniprot | |
| S1124 | Phosphorylation | Uniprot | |
| S1131 | Phosphorylation | Uniprot | |
| T1284 | O-Glycosylation | Uniprot | |
| S1287 | Phosphorylation | Uniprot | |
| S1296 | Phosphorylation | Uniprot | |
| S1299 | Phosphorylation | Uniprot | |
| S1300 | Phosphorylation | Uniprot | |
| Y1395 | Phosphorylation | Uniprot | |
| N1421 | N-Glycosylation | Uniprot |
研究背景
May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.
The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies.
The intracellular/endo domain is disulfide-linked.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
The ectodomain is shedded from the surface of keratinocytes resulting in a 120-kDa soluble form, also named as 120 kDa linear IgA disease antigen. The shedding is mediated by membrane-bound metalloproteases. This cleavage is inhibited by phosphorylation at Ser-544.
Cell junction>Hemidesmosome. Membrane>Single-pass type II membrane protein.
Note: Localized along the plasma membrane of the hemidesmosome.
Secreted>Extracellular space>Extracellular matrix>Basement membrane.
Note: Exclusively localized to anchoring filaments. Localized to the epidermal side of split skin.
Secreted>Extracellular space>Extracellular matrix>Basement membrane.
Note: Localized in the lamina lucida beneath the hemidesmosomes.
Detected in skin. In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells (at protein level). Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.
Homotrimers of alpha 1(XVII)chains. Interacts (via cytoplasmic region) with ITGB4 (via cytoplasmic region). Interacts (via cytoplasmic region) with DST isoform 3 (via N-terminus). Interacts (via N-terminus) with PLEC. Interacts (via cytoplasmic region) with DSP.
研究领域
· Organismal Systems > Digestive system > Protein digestion and absorption.
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