Cytochrome P450 2B6 Antibody - #AF0637

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产品: Cytochrome P450 2B6 抗体
货号: AF0637
描述: Rabbit polyclonal antibody to Cytochrome P450 2B6
应用: WB IHC IF/ICC
反应: Human, Monkey
分子量: 56kDa; 56kD(Calculated).
蛋白号: P20813
RRID: AB_2834379

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Monkey
克隆:
Polyclonal
特异性:
Cytochrome P450 2B6 Antibody detects endogenous levels of total Cytochrome P450 2B6.
RRID:
AB_2834379
引用格式: Affinity Biosciences Cat# AF0637, RRID:AB_2834379.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1,4 cineole 2 exo monooxygenase; 11B1; CP2B6_HUMAN; CPB 6; CPB6; CYP 2B6; CYP IIB6; CYP2B; CYP2B6; CYP2B7; CYP2B7P; CYPIIB6; Cytochrome P450 2B6; Cytochrome P450 family 2 subfamily B; Cytochrome P450 family 2 subfamily B polypeptide 6; Cytochrome P450 IIB1; Cytochrome P450 phenobarbital inducible; Cytochrome P450 subfamily IIB (phenobarbital inducible); Cytochrome P450 subfamily IIB (phenobarbital inducible) polypeptide 6; Cytochrome P450 subfamily IIB polypeptide 6; EFVM; IIB1; P450; P450 IIB1; P450IIB1;

抗原和靶标

免疫原:
Uniprot:

P20813(CP2B6_HUMAN) >>Visit HPA database.

基因/基因ID:
CYP2B6(1555)
表达:
P20813 CP2B6_HUMAN:

Expressed in liver, lung and heart right ventricle.

描述:
This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.
序列:
MELSVLLFLALLTGLLLLLVQRHPNTHDRLPPGPRPLPLLGNLLQMDRRGLLKSFLRFREKYGDVFTVHLGPRPVVMLCGVEAIREALVDKAEAFSGRGKIAMVDPFFRGYGVIFANGNRWKVLRRFSVTTMRDFGMGKRSVEERIQEEAQCLIEELRKSKGALMDPTFLFQSITANIICSIVFGKRFHYQDQEFLKMLNLFYQTFSLISSVFGQLFELFSGFLKYFPGAHRQVYKNLQEINAYIGHSVEKHRETLDPSAPKDLIDTYLLHMEKEKSNAHSEFSHQNLNLNTLSLFFAGTETTSTTLRYGFLLMLKYPHVAERVYREIEQVIGPHRPPELHDRAKMPYTEAVIYEIQRFSDLLPMGVPHIVTQHTSFRGYIIPKDTEVFLILSTALHDPHYFEKPDAFNPDHFLDANGALKKTEAFIPFSLGKRICLGEGIARAELFLFFTTILQNFSMASPVAPEDIDLTPQECGVGKIPPTYQIRFLPR

翻译修饰 - P20813 作为底物

Site PTM Type Enzyme
S128 Phosphorylation P17612 (PRKACA)
K433 Acetylation

研究背景

功能:

A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling. Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2. Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs. Acts as a 1,4-cineole 2-exo-monooxygenase.

Allele 2B6*9: Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.

翻译修饰:

Phosphorylation is accompanied by a decrease in enzyme activity.

细胞定位:

Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in liver, lung and heart right ventricle.

蛋白家族:

Belongs to the cytochrome P450 family.

研究领域

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Constitutive androstane receptor weakens the induction of panaxytriol on CYP3A4 by repressing the activation of pregnane X receptor. BIOCHEMICAL PHARMACOLOGY, 2019 (PubMed: 30414935) [IF=5.8]

Application: WB    Species: human    Sample: HepG2 cells

Fig.2| Effect of PXT on CYP2B6 mRNA and protein expression in HepG2 cells.The effect of PXT on CYP2B6 mRNA and protein in HepG2 cells at a series of concentrations (5, 10, 20, 40, and 80 μM) for 24h was determined by real-time Q-PCR analysis (A) and Western blot analysis (B-C). The determination of mRNA and protein was normalized to GAPDH and β-actin, respectively. CITCO (10 μM) and 0.1% DMSO were used as the positive and negative controls, respectively. Results are presented as fold induction relative to control, and all data are expressed mean ± SD of triplicate independent experiments.

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