Occludin Antibody | Affinity Biosciences LTD|亲科生物官网

产品:	Occludin 抗体
货号:	DF7504
描述:	Rabbit polyclonal antibody to Occludin
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat, Pig
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量:	23~30kD, 52~65kD; 59kD(Calculated).
蛋白号:	Q16625
RRID:	AB_2841004

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat,Pig

预测:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)

克隆:

Polyclonal

特异性:

Occludin Antibody detects endogenous levels of total Occludin.

RRID:

AB_2841004
引用格式: Affinity Biosciences Cat# DF7504, RRID:AB_2841004.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; Ocln; OCLN_HUMAN; Tight junction protein occludin;

抗原和靶标

免疫原:

Uniprot:

Q16625(OCLN_HUMAN) >>Visit HPA database.

基因/基因ID:

OCLN(100506658)

表达:

Q16625 OCLN_HUMAN:

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

序列:

Q16625 OCLN_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGGSVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFIAALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPTAQSSGSLYGSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q16625 作为底物

Q16625

Site	PTM Type	Enzyme	Source
S8	Phosphorylation		Uniprot
K18	Ubiquitination		Uniprot
S40	Phosphorylation		Uniprot
Y44	Phosphorylation		Uniprot
S45	Phosphorylation		Uniprot
K276	Ubiquitination		Uniprot
S277	Phosphorylation		Uniprot
K283	Ubiquitination		Uniprot
Y287	Phosphorylation		Uniprot
K299	Ubiquitination		Uniprot
S302	Phosphorylation		Uniprot
T305	Phosphorylation		Uniprot
S310	Phosphorylation		Uniprot
S313	Phosphorylation		Uniprot
Y315	Phosphorylation		Uniprot
S321	Phosphorylation		Uniprot
Y325	Phosphorylation		Uniprot
S326	Phosphorylation		Uniprot
S327	Phosphorylation		Uniprot
K330	Ubiquitination		Uniprot
Y337	Phosphorylation		Uniprot
S340	Phosphorylation		Uniprot
S341	Phosphorylation		Uniprot
Y342	Phosphorylation		Uniprot
K343	Ubiquitination		Uniprot
S344	Phosphorylation		Uniprot
T345	Phosphorylation		Uniprot
S358	Phosphorylation		Uniprot
Y368	Phosphorylation		Uniprot
S369	Phosphorylation		Uniprot
S370	Phosphorylation		Uniprot
T376	Phosphorylation		Uniprot
S378	Phosphorylation		Uniprot
K379	Ubiquitination		Uniprot
T393	Phosphorylation		Uniprot
Y398	Phosphorylation	P12931 (SRC)	Uniprot
T400	Phosphorylation	P68400 (CSNK2A1)	Uniprot
Y402	Phosphorylation	P12931 (SRC)	Uniprot
T403	Phosphorylation	Q02156 (PRKCE) , P24723 (PRKCH) , Q05513 (PRKCZ)	Uniprot
T404	Phosphorylation	Q02156 (PRKCE) , Q05513 (PRKCZ) , P24723 (PRKCH) , P68400 (CSNK2A1) , P67870 (CSNK2B)	Uniprot
S408	Phosphorylation	P67870 (CSNK2B) , P68400 (CSNK2A1)	Uniprot
T424	Phosphorylation	Q02156 (PRKCE) , Q05513 (PRKCZ)	Uniprot
T438	Phosphorylation	Q02156 (PRKCE) , Q05513 (PRKCZ)	Uniprot
Y443	Phosphorylation		Uniprot
S445	Phosphorylation		Uniprot
S448	Phosphorylation		Uniprot
S458	Phosphorylation		Uniprot
Y467	Phosphorylation		Uniprot
S471	Phosphorylation		Uniprot
Y474	Phosphorylation		Uniprot
Y481	Phosphorylation		Uniprot
K488	Ubiquitination		Uniprot
S490	Phosphorylation	P05771 (PRKCB)	Uniprot

研究背景

Q16625_OCLN_HUMAN

功能:

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.

翻译修饰:

Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.

细胞定位:

Cell membrane>Multi-pass membrane protein. Cell junction>Tight junction.

组织特异性:

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

亚基结构:

Interacts with TJP1/ZO1. Interacts with VAPA. Interacts with CLDN1, CLDN6, CLDN9, CLDN11, CLDN12 and CLDN17.

蛋白家族:

The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.

Belongs to the ELL/occludin family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

文献引用

1). Gingerenone A Attenuates Ulcerative Colitis via Targeting IL-17RA to Inhibit Inflammation and Restore Intestinal Barrier Function. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38639442) [IF=15.1]

2). Extracellular vesicles of Fusobacterium nucleatum compromise intestinal barrier through targeting RIPK1-mediated cell death pathway. Gut Microbes, 2021 (PubMed: 33769187) [IF=12.2]

Application: IHC Species: mouse Sample: colon

Figure 5. |FnEVs increase gut barrier leakage in experimental colitis models. (a) Representative images and ex vivo imaging with the intestine, liver, heart, spleen and kidney of mice. (b) Relative fluorescence intensity of translocated EGFP-labeled E.coli in every tissues. (c) Representative images of immunohistochemical stainings of ZO-1, claudin-1 and occludin in the colon on day 3 after colitis induction. Scale bar = 50 um.

3). Water-extracted Lonicera japonica polysaccharide attenuates allergic rhinitis by regulating NLRP3-IL-17 signaling axis. Carbohydrate Polymers, 2022 (PubMed: 36184153) [IF=11.2]

4). Low dose of arsenic exacerbates toxicity to mice and IPEC-J2 cells exposed with deoxynivalenol: Aryl hydrocarbon receptor and autophagy might be novel therapeutic targets. Science of The Total Environment, 2022 (PubMed: 35381244) [IF=9.8]

5). Multistage-Responsive Nanocomplexes Attenuate Ulcerative Colitis by Improving the Accumulation and Distribution of Oral Nucleic Acid Drugs in the Colon. ACS Applied Materials & Interfaces, 2022 (PubMed: 34978415) [IF=9.5]

6). Fourteen composite probiotics alleviate type 2 diabetes through modulating gut microbiota and modifying M1/M2 phenotype macrophage in db/ db mice. PHARMACOLOGICAL RESEARCH, 2020 (PubMed: 32818655) [IF=9.3]

Application: IHC Species: mouse Sample: colon

Fig. 4.| Effects of the composite probiotics on intestinal barrier function.The protein expression of claudin-1 (G), occludin-1 (H), ZO-1 (I) and mucin-2 (J)determined by immunohistochemistry (original magnification 200×) (n = 4 images/group).

7). Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect: impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway. PHARMACOLOGICAL RESEARCH, 2020 (PubMed: 31863867) [IF=9.3]

Application: WB Species: Mice Sample: colonic tissues

Fig. 4. OBB protected intestinal epithelial barrier by modulating TJs proteins. Effect of OBB on the mRNA levels of mucin-1(A) and mucin-2 (B). (C) Representative Western blotting images of TJs protein, and the relative protein expressions were normalized to β-actin. (D-H) Changes in the relative protein expression levels of ZO-1, ZO-2, occludin, JAM-A, and claudin-1 were measured respectively. Data are shown as mean ± SEM (n = 3). # P < 0.05, ## P < 0.01 vs. Control group, * P < 0.05, ** P < 0.01 vs. DSS group, & P < 0.05, && P < 0.01 vs. BBR group.

8). Gut microbiota-mediated secondary bile acid alleviates Staphylococcus aureus-induced mastitis through the TGR5-cAMP-PKA-NF-κB/NLRP3 pathways in mice. npj Biofilms and Microbiomes, 2023 (PubMed: 36755021) [IF=9.2]

9). Gut microbiome determines therapeutic effects of OCA on NAFLD by modulating bile acid metabolism. npj Biofilms and Microbiomes, 2023 (PubMed: 37258543) [IF=9.2]

10). Vitamin K1 ameliorates lipopolysaccharide-triggered skeletal muscle damage revealed by faecal bacteria transplantation. Journal of cachexia, sarcopenia and muscle, 2024 (PubMed: 38018317) [IF=8.9]

Application: WB Species: Mouse Sample:

Figure 2 Faecal bacteria transplantation from S‐res mice alleviates LPS‐triggered muscle damage and intestinal barrier disruption. (A) Description of the steps of FMT experiment. (B) The score for Severity of sepsis in Sen and Res. (C) H&E staining in the GA muscle. Representative histological images on day 9 postoperatively (400× magnification). Scale bar = 100 μm. Comparison of muscle fibre diameter and CSA of muscle fibre in mice from each group. (D) Western blot analysis of MuRF‐1 and MAFbx protein levels in TA tissue in Sen and Res groups. The results were normalized to the expression of GAPDH. (E) Western blot analysis of ZO‐1 and occludin protein levels in colon tissue in Sen and Res groups. The results were normalized to the expression of GAPDH. Data are means ± SEM. P values determined through Student's t test and ordinary one‐way ANOVA. Sen versus Res: # P < 0.05, ## P < 0.01, ### P < 0.001. Sen and Res versus Ctrl: **P < 0.01, ***P < 0.001. Sen versus ABX: $ P < 0.05.

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Occludin Antibody - #DF7504

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