(3)
(5)
产品描述
来源:
Rabbit
应用:
WB 1:500-3000, IHC 1:50-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%)
克隆:
Polyclonal
特异性:
TRPM7 Antibody detects endogenous levels of total TRPM7.
RRID:
AB_2841012
引用格式: Affinity Biosciences Cat# DF7513, RRID:AB_2841012.
引用格式: Affinity Biosciences Cat# DF7513, RRID:AB_2841012.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
ALSPDC; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; Transient receptor potential cation channel subfamily M member 7; TRP PLIK; TRPM7; TRPM7_HUMAN;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
序列:
- Q96QT4 TRPM7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLAMKYSDVKLGDHFNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEVILQLLLKEWQMELPKLVISVHGGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVIENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTIKKTFNFGQNEALHLFQTLMECMKRKELITVFHIGSDEHQDIDVAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVAFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLFHLVRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRKSHESFGNRADKKEKMRHNHFIKTAQPYRPKIDTVMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWACLMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPAILLLEYKTKAEMSHIPQSQDAHQMTMDDSENNFQNITEEIPMEVFKEVRILDSNEGKNEMEIQMKSKKLPITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKVREIFMSEAGKVNQKIKVWFSDYFNISDTIAIISFFIGFGLRFGAKWNFANAYDNHVFVAGRLIYCLNIIFWYVRLLDFLAVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEAPSWTLAKDIVFHPYWMIFGEVYAYEIDVCANDSVIPQICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIMAYHEKPVLPPPLIILSHIVSLFCCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFNEKDDKFHSGSEERIRVTFERVEQMCIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKVHNEITRELSISKHLAQNLIDDGPVRPSVWKKHGVVNTLSSSLPQGDLESNNPFHCNILMKDDKDPQCNIFGQDLPAVPQRKEFNFPEAGSSSGALFPSAVSPPELRQRLHGVELLKIFNKNQKLGSSSTSIPHLSSPPTKFFVSTPSQPSCKSHLETGTKDQETVCSKATEGDNTEFGAFVGHRDSMDLQRFKETSNKIKILSNNNTSENTLKRVSSLAGFTDCHRTSIPVHSKQAEKISRRPSTEDTHEVDSKAALIPDWLQDRPSNREMPSEEGTLNGLTSPFKPAMDTNYYYSAVERNNLMRLSQSIPFTPVPPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAKIEFLSKEEMGGGLRRAVKVQCTWSEHDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVLHLCLREIQQQRAAQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLKRNDYTPDKIIFPQDEPSDLNLQPGNSTKESESTNSVRLML
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Rabbit
100
Pig
100
Horse
88
Bovine
88
Sheep
88
Dog
88
Xenopus
75
Chicken
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96QT4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| T12 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| T523 | Phosphorylation | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| K667 | Ubiquitination | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| Y776 | Phosphorylation | Uniprot | |
| T778 | Phosphorylation | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| S907 | Phosphorylation | Uniprot | |
| K1159 | Ubiquitination | Uniprot | |
| T1163 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| K1168 | Acetylation | Uniprot | |
| S1191 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1193 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1230 | Phosphorylation | Uniprot | |
| S1239 | Phosphorylation | Uniprot | |
| K1253 | Ubiquitination | Uniprot | |
| S1255 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1258 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1265 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1269 | Phosphorylation | Uniprot | |
| S1271 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1287 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1358 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1361 | Phosphorylation | Uniprot | |
| S1386 | Phosphorylation | Uniprot | |
| S1387 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1390 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1395 | Phosphorylation | Uniprot | |
| S1396 | Phosphorylation | Uniprot | |
| S1404 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1405 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1407 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1410 | Phosphorylation | Uniprot | |
| S1413 | Phosphorylation | Uniprot | |
| T1417 | Phosphorylation | Uniprot | |
| T1419 | Phosphorylation | Uniprot | |
| T1435 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1446 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1455 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1456 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1463 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1468 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1471 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| K1473 | Methylation | Uniprot | |
| K1473 | Ubiquitination | Uniprot | |
| S1476 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1477 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1482 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1488 | Phosphorylation | Uniprot | |
| S1493 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1500 | Phosphorylation | Uniprot | |
| S1504 | Phosphorylation | Uniprot | |
| T1505 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1508 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1513 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1527 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1533 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1537 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1542 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1543 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1551 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1567 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1569 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1583 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1598 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1615 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1660 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1685 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1695 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1779 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1830 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| K1833 | Ubiquitination | Uniprot | |
| S1851 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1860 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
翻译修饰 - Q96QT4 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O00418 (EEF2K) | S78 | Uniprot |
| P04083 (ANXA1) | S5 | Uniprot |
| P28289 (TMOD1) | S2 | Uniprot |
| P28289 (TMOD1) | T54 | Uniprot |
| P35579 (MYH9) | T1800 | Uniprot |
| P35579 (MYH9) | S1803 | Uniprot |
| P35579 (MYH9) | S1808 | Uniprot |
| Q96QT4 (TRPM7) | T1163 | Uniprot |
| Q96QT4 (TRPM7) | S1191 | Uniprot |
| Q96QT4 (TRPM7) | S1193 | Uniprot |
| Q96QT4 (TRPM7) | S1255 | Uniprot |
| Q96QT4 (TRPM7) | S1258 | Uniprot |
| Q96QT4 (TRPM7) | T1265 | Uniprot |
| Q96QT4 (TRPM7) | S1271 | Uniprot |
| Q96QT4 (TRPM7) | S1287 | Uniprot |
| Q96QT4 (TRPM7) | S1358 | Uniprot |
| Q96QT4 (TRPM7) | S1387 | Uniprot |
| Q96QT4 (TRPM7) | S1390 | Uniprot |
| Q96QT4 (TRPM7) | S1404 | Uniprot |
| Q96QT4 (TRPM7) | T1405 | Uniprot |
| Q96QT4 (TRPM7) | S1407 | Uniprot |
| Q96QT4 (TRPM7) | T1435 | Uniprot |
| Q96QT4 (TRPM7) | S1446 | Uniprot |
| Q96QT4 (TRPM7) | T1455 | Uniprot |
| Q96QT4 (TRPM7) | S1456 | Uniprot |
| Q96QT4 (TRPM7) | S1463 | Uniprot |
| Q96QT4 (TRPM7) | S1468 | Uniprot |
| Q96QT4 (TRPM7) | T1471 | Uniprot |
| Q96QT4 (TRPM7) | S1476 | Uniprot |
| Q96QT4 (TRPM7) | S1477 | Uniprot |
| Q96QT4 (TRPM7) | T1482 | Uniprot |
| Q96QT4 (TRPM7) | S1493 | Uniprot |
| Q96QT4 (TRPM7) | T1505 | Uniprot |
| Q96QT4 (TRPM7) | T1508 | Uniprot |
| Q96QT4 (TRPM7) | S1513 | Uniprot |
| Q96QT4 (TRPM7) | S1527 | Uniprot |
| Q96QT4 (TRPM7) | S1533 | Uniprot |
| Q96QT4 (TRPM7) | T1537 | Uniprot |
| Q96QT4 (TRPM7) | T1542 | Uniprot |
| Q96QT4 (TRPM7) | S1543 | Uniprot |
| Q96QT4 (TRPM7) | T1551 | Uniprot |
| Q96QT4 (TRPM7) | S1567 | Uniprot |
| Q96QT4 (TRPM7) | S1569 | Uniprot |
| Q96QT4 (TRPM7) | T1583 | Uniprot |
| Q96QT4 (TRPM7) | S1598 | Uniprot |
| Q96QT4 (TRPM7) | S1615 | Uniprot |
| Q96QT4 (TRPM7) | S1660 | Uniprot |
| Q96QT4 (TRPM7) | T1685 | Uniprot |
| Q96QT4 (TRPM7) | S1695 | Uniprot |
| Q96QT4 (TRPM7) | S1779 | Uniprot |
| Q96QT4 (TRPM7) | T1830 | Uniprot |
| Q96QT4 (TRPM7) | S1851 | Uniprot |
| Q96QT4 (TRPM7) | S1860 | Uniprot |
研究背景
功能:
Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
翻译修饰:
Autophosphorylated.
细胞定位:
Membrane>Multi-pass membrane protein.
亚基结构:
Homodimer. Interacts with PLCB1 (By similarity). Forms heterodimers with TRPM6.
蛋白家族:
In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Digestive system > Mineral absorption.
文献引用
1). Phosphorus magnesium fiber regulates macrophage polarization through TRPM7 to accelerate wound healing. Applied Materials Today, 2023
[IF=8.3]
Application: WB Species: Mouse Sample:
Fig. 4. PMF promoted wound healing through TRPM7-ERK1/2. (A) The release content and schematic diagram of magnesium ions in the wound site on the 3rd, 7th, and 15th days. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group, n = 3 (B) Western blot analysis of the expression of TRPM7 and phosphorylated ERK1/2 proteins. (C) Quantitative analysis of related protein expression levels in the PMF treatment group compared with the control group. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3. (D) Immunofluorescent staining images of skin tissue sections on day 7. CD68: pan-macrophage marker (red). TRPM7: (green). Scale bar: 40 μm. (E) Statistical summary of fluorescent expressing of TRPM7/CD68 expressing. ns: no significance
Application: IF/ICC Species: Mouse Sample:
Fig. 4. PMF promoted wound healing through TRPM7-ERK1/2. (A) The release content and schematic diagram of magnesium ions in the wound site on the 3rd, 7th, and 15th days. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group, n = 3 (B) Western blot analysis of the expression of TRPM7 and phosphorylated ERK1/2 proteins. (C) Quantitative analysis of related protein expression levels in the PMF treatment group compared with the control group. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3. (D) Immunofluorescent staining images of skin tissue sections on day 7. CD68: pan-macrophage marker (red). TRPM7: (green). Scale bar: 40 μm. (E) Statistical summary of fluorescent expressing of TRPM7/CD68 expressing. ns: no significance
2). Gallium ions promote osteoinduction of human and mouse osteoblasts via the TRPM7/Akt signaling pathway. Molecular Medicine Reports, 2020
(PubMed: 32945378)
[IF=3.4]
Application: WB Species: human Sample: MC3T3 cells
Figure 3. |Effect of TRPM7 siRNAs on TRPM7 protein expression in MC3T3‑E1 and hFOB1.19 cells. (A) Total and (B) membrane TRPM7 protein expression levels in MC3T3 cells following transfection with TRPM7 siRNAs.
3). Novel benzoylurea derivative decreases TRPM7 channel function and inhibits cancer cells migration. Channels (Austin, Tex.), 2024
(PubMed: 39212541)
[IF=3.3]
Application: WB Species: Human Sample: MCF-7 cells
Figure 4. SUD inhibits TRPM7 expression through PI3K/Akt signaling pathway. (a) Effects of different concentrations SUD on the AKT1 and PI3K mRNA expression in MCF-7 cells examined by qPCR. (b) effects of different concentrations SUD on the AKT1 and PI3K mRNA expression in BGC-823 cells examined by qPCR. ***, **** denote significant difference with control group, p
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