| 产品: | DVL1 抗体 |
| 货号: | DF7515 |
| 描述: | Rabbit polyclonal antibody to DVL1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat, Monkey |
| 预测: | Pig, Bovine, Horse, Sheep, Dog, Chicken |
| 分子量: | 75 kDa; 75kD(Calculated). |
| 蛋白号: | O14640 |
| RRID: | AB_2841014 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7515, RRID:AB_2841014.
展开/折叠
Dishevelled 1; dishevelled 1 like; Dishevelled; Dishevelled dsh homolog 1; Dishevelled-1; Dishevelled1; DSH homolog 1; Dvl 1; Dvl; Dvl1; DVL1_HUMAN; DVL1L1; MGC54245; Segment polarity protein dishevelled homolog DVL 1; segment polarity protein dishevelled homolog DVL 1 like; Segment polarity protein dishevelled homolog DVL-1; Segment polarity protein dishevelled homolog DVL1;
抗原和靶标
- O14640 DVL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKLPCFNGRVVSWLVLAEGAHSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGTESMVSHRRERARRRNREEAARTNGHPRGDRRRDVGLPPDSASTALSSELESSSFVDSDEDGSTSRLSSSTEQSTSSRLIRKHKRRRRKQRLRQADRASSFSSITDSTMSLNIVTVTLNMERHHFLGISIVGQSNDRGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDVNFENMSNDDAVRVLREIVSQTGPISLTVAKCWDPTPRSYFTVPRADPVRPIDPAAWLSHTAALTGALPRYGTSPCSSAVTRTSSSSLTSSVPGAPQLEEAPLTVKSDMSAVVRVMQLPDSGLEIRDRMWLKITIANAVIGADVVDWLYTHVEGFKERREARKYASSLLKHGFLRHTVNKITFSEQCYYVFGDLCSNLATLNLNSGSSGTSDQDTLAPLPHPAAPWPLGQGYPYQYPGPPPCFPPAYQDPGFSYGSGSTGSQQSEGSKSSGSTRSSRRAPGREKERRAAGAGGSGSESDHTAPSGVGSSWRERPAGQLSRGSSPRSQASATAPGLPPPHPTTKAYTVVGGPPGGPPVRELAAVPPELTGSRQSFQKAMGNPCEFFVDIM
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14640 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| Ubiquitination | Uniprot | ||
| Y8 | Phosphorylation | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| T100 | Phosphorylation | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| S142 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| S188 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| T348 | Phosphorylation | Uniprot | |
| S390 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| S604 | Phosphorylation | Uniprot | |
| S625 | Phosphorylation | Uniprot | |
| R626 | Methylation | Uniprot | |
| Y651 | Phosphorylation | Uniprot | |
| T674 | Phosphorylation | Uniprot | |
| S676 | Phosphorylation | Uniprot | |
| S679 | Phosphorylation | Uniprot |
研究背景
Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).
Ubiquitinated; undergoes both 'Lys-48'-linked ubiquitination, leading to its subsequent degradation by the ubiquitin-proteasome pathway, and 'Lys-63'-linked ubiquitination. The interaction with INVS is required for ubiquitination. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains (By similarity).
Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytosol. Cytoplasmic vesicle.
Note: Localizes at the cell membrane upon interaction with frizzled family members.
Interacts with CXXC4. Interacts (via PDZ domain) with NXN (By similarity). Interacts with BRD7 and INVS. Interacts (via PDZ domain) with VANGL1 and VANGL2 (via C-terminus). Interacts with ARRB1; the interaction is enhanced by phosphorylation of DVL1. Interacts with CYLD (By similarity). Interacts (via PDZ domain) with RYK. Self-associates (via DIX domain) and forms higher homooligomers. Interacts (via PDZ domain) with DACT1 and FZD7, where DACT1 and FZD7 compete for the same binding site (By similarity). Interacts (via DEP domain) with MUSK; the interaction is direct and mediates the formation a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering (By similarity). Interacts (via PDZ domain) with TMEM88. Interacts with DCDC2. Interacts with FOXK2. Interacts with PKD1 (via extracellular domain). Interacts (via PDZ domain) with CCDC88C/DAPLE; competes with CCDC88C for binding to frizzled receptor FZD7 and dissociates from CCDC88C following initiation of non-canonical Wnt signaling when CCDC88C displaces DVL1 from ligand-activated FZD7.
The DIX domain promotes homooligomerization.
The DEP domain mediates interaction with the cell membrane.
Belongs to the DSH family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Notch signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Basal cell carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)
· Organismal Systems > Endocrine system > Melanogenesis.
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