| 产品: | CCDC99 抗体 |
| 货号: | AF0628 |
| 描述: | Rabbit polyclonal antibody to CCDC99 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 70kDa; 70kD(Calculated). |
| 蛋白号: | Q96EA4 |
| RRID: | AB_2834382 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0628, RRID:AB_2834382.
展开/折叠
AA409762; Arsenite related gene 1 protein; Arsenite-related gene 1 protein; CCDC 99; Ccdc99; Coiled coil domain containing 99; Coiled-coil domain-containing protein 99; FLJ20364; FLJ40690; hSpindly; MGC124977; Protein Spindly; rhabdomyosarcoma antigen MU RMS 40.4A; Rhabdomyosarcoma antigen MU-RMS-40.4A; Rrhabdomyosarcoma antigen protein MU RMS 40.4A; SPDL1; SPDLY_HUMAN; Spindle apparatus coiled coil domain containing protein 1; Spindle apparatus coiled coil protein 1; Spindly; Spindly homolog;
抗原和靶标
- Q96EA4 SPDLY_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEADIITNLRCRLKEAEEERLKAAQYGLQLVESQNELQNQLDKCRNEMMTMTESYEQEKYTLQREVELKSRMLESLSCECEAIKQQQKMHLEKLEEQLSRSHGQEVNELKTKIEKLKVELDEARLSEKQLKHQVDHQKELLSCKSEELRVMSERVQESMSSEMLALQIELTEMESMKTTLKEEVNELQYRQEQLELLITNLMRQVDRLKEEKEEREKEAVSYYNALEKARVANQDLQVQLDQALQQALDPNSKGNSLFAEVEDRRAAMERQLISMKVKYQSLKKQNVFNREQMQRMKLQIATLLQMKGSQTEFEQQERLLAMLEQKNGEIKHLLGEIRNLEKFKNLYDSMESKPSVDSGTLEDNTYYTDLLQMKLDNLNKEIESTKGELSIQRMKALFESQRALDIERKLFANERCLQLSESENMKLRAKLDELKLKYEPEETVEVPVLKKRREVLPVDITTAKDACVNNSALGGEVYRLPPQKEETQSCPNSLEDNNLQLEKSVSIYTPVVSLSPHKNLPVDMQLKKEKKCVKLIGVPADAEALSERSGNTPNSPRLAAESKLQTEVKEGKETSSKLEKETCKKLHPILYVSSKSTPETQCPQQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96EA4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| R154 | Methylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| Y347 | Phosphorylation | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| T461 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| T487 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| T509 | Phosphorylation | Uniprot | |
| S513 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S515 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S546 | Phosphorylation | Uniprot | |
| S555 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| T566 | Phosphorylation | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| T600 | Phosphorylation | Uniprot |
研究背景
Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track).
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Chromosome>Centromere>Kinetochore. Nucleus. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
Interacts with KNTC1 and ZW10. These interactions appear weak and may be transient or indirect. Interacts with dynein intermediate chain and dynactin (DCTN1).
Belongs to the Spindly family.
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