| 产品: | RNF14 抗体 |
| 货号: | AF0819 |
| 描述: | Rabbit polyclonal antibody to RNF14 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 50kDa; 54kD(Calculated). |
| 蛋白号: | Q9UBS8 |
| RRID: | AB_2834384 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0819, RRID:AB_2834384.
展开/折叠
Androgen receptor associated protein 54; Androgen receptor-associated protein 54; ARA 54; ARA54; E3 ubiquitin protein ligase RNF14; E3 ubiquitin-protein ligase RNF14; FLJ26004; HFB 30; HFB30; HRIHFB2038; RING finger protein 14; RNF 14; Rnf14; RNF14_HUMAN; TRIAD 2; TRIAD2; Triad2 protein;
抗原和靶标
- Q9UBS8 RNF14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSEDREAQEDELLALASIYDGDEFRKAESVQGGETRIYLDLPQNFKIFVSGNSNECLQNSGFEYTICFLPPLVLNFELPPDYPSSSPPSFTLSGKWLSPTQLSALCKHLDNLWEEHRGSVVLFAWMQFLKEETLAYLNIVSPFELKIGSQKKVQRRTAQASPNTELDFGGAAGSDVDQEEIVDERAVQDVESLSNLIQEILDFDQAQQIKCFNSKLFLCSICFCEKLGSECMYFLECRHVYCKACLKDYFEIQIRDGQVQCLNCPEPKCPSVATPGQVKELVEAELFARYDRLLLQSSLDLMADVVYCPRPCCQLPVMQEPGCTMGICSSCNFAFCTLCRLTYHGVSPCKVTAEKLMDLRNEYLQADEANKRLLDQRYGKRVIQKALEEMESKEWLEKNSKSCPCCGTPIEKLDGCNKMTCTGCMQYFCWICMGSLSRANPYKHFNDPGSPCFNRLFYAVDVDDDIWEDEVED
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UBS8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K27 | Ubiquitination | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| Y242 | Phosphorylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| Y379 | Phosphorylation | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K399 | Ubiquitination | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| T409 | Phosphorylation | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| S451 | Phosphorylation | Uniprot |
研究背景
Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.
RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.
Cytoplasm. Nucleus.
Widely expressed.
Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2. Interacts with AR/androgen receptor; testosterone- and RNF6-regulated it promotes AR transcriptional activity.
The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
The RING-type zinc finger is essential for the interaction with UBE2E2.
Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.
Belongs to the RBR family. RNF14 subfamily.
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