ASC2 Antibody - #DF7540

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产品: ASC2 抗体
货号: DF7540
描述: Rabbit polyclonal antibody to ASC2
应用: WB IHC
反应: Human, Mouse, Rat
分子量: 10 kDa; 10kD(Calculated).
蛋白号: Q8WXC3
RRID: AB_2841039

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
ASC2 Antibody detects endogenous levels of total ASC2.
RRID:
AB_2841039
引用格式: Affinity Biosciences Cat# DF7540, RRID:AB_2841039.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

PAAD domain only protein; PAAD-only protein 1; POP1; PYC1; PYD (pyrin domain) containing 1; PYDC1; PYDC1_HUMAN; Pyrin domain containing 1; Pyrin domain-containing protein 1; Pyrin only protein 1; Pyrin-only protein 1;

抗原和靶标

免疫原:
Uniprot:

Q8WXC3(PYDC1_HUMAN) >>Visit HPA database.

基因/基因ID:
PYDC1(260434)
表达:
Q8WXC3 PYDC1_HUMAN:

Predominantly expressed in monocytes, macrophages and granulocytes.

序列:
MGTKREAILKVLENLTPEELKKFKMKLGTVPLREGFERIPRGALGQLDIVDLTDKLVASYYEDYAAELVVAVLRDMRMLEEAARLQRAA

研究背景

功能:

Associates with PYCARD/ASC and modulates its ability to collaborate with MEFV/pyrin and NLRP3/cryopyrin in NF-kappa-B and pro-caspase-1 activation. Suppresses kinase activity of NF-kappa-B inhibitor kinase (IKK) complex, expression of NF-kappa-B inducible genes and inhibits NF-kappa-B activation by cytokines and LPS.

翻译修饰:

Phosphorylated.

细胞定位:

Cytoplasm.
Note: Recruited to specks formed by PYCARD within the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Predominantly expressed in monocytes, macrophages and granulocytes.

亚基结构:

Interacts with PYCARD/ASC (via pyrin domain).

研究领域

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). lncRNA‑MALAT1 promotes high glucose‑induced H9C2 cardiomyocyte pyroptosis by downregulating miR‑141‑3p expression. Molecular Medicine Reports, 2021 (PubMed: 33576445) [IF=3.4]

Application: WB    Species: Human    Sample: H9C2 cells

Figure 3. Effects of MALAT1 on pyroptosis-associated protein expression levels. ASC, GSDMD-N, caspase-1, NLRP3 and GSDMD protein expression levels were measured via western blotting. *P<0.05 and **P<0.01 vs. NG; #P<0.05 and ##P<0.01 vs. HG; ^P<0.05 and ^^P<0.01 vs. NC1; %P<0.05 vs. NC2. MALAT1, metastasis-associated lung adenocarcinoma transcript-1; ASC, apoptosis-associated speck-like protein; GSDMD, gasdermin D; NLRP3, nucleotide oligomerization domain-like receptor protein 3; NG, normal glucose; HG, high glucose; NC, negative control; NC1, MALAT1 overexpression NC group; NC2, MALAT1 knockdown NC group; si, small interfering RNA.

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