产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0657, RRID:AB_2834385.
展开/折叠
KIAA0161; Probable E3 ubiquitin protein ligase RNF144A; Probable E3 ubiquitin-protein ligase RNF144A; R144A_HUMAN; Ring finger protein 144; RING finger protein 144A; RNF 144; RNF 144A; rnf144a; UBCE7IP4; UbcM4 interacting protein 4; UbcM4-interacting protein 4; Ubiquitin conjugating enzyme 7 interacting protein 4; Ubiquitin-conjugating enzyme 7-interacting protein 4;
抗原和靶标
- P50876 R144A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTTRYRPTWDLALDPLVSCKLCLGEYPVEQMTTIAQCQCIFCTLCLKQYVELLIKEGLETAISCPDAACPKQGHLQENEIECMVAAEIMQRYKKLQFEREVLFDPCRTWCPASTCQAVCQLQDVGLQTPQPVQCKACRMEFCSTCKASWHPGQGCPETMPITFLPGETSAAFKMEEDDAPIKRCPKCKVYIERDEGCAQMMCKNCKHAFCWYCLESLDDDFLLIHYDKGPCRNKLGHSRASVIWHRTQVVGIFAGFGLLLLVASPFLLLATPFVLCCKCKCSKGDDDPLPT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P50876 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K183 | Ubiquitination | Uniprot | |
| K187 | Sumoylation | Uniprot | |
| K189 | Sumoylation | Uniprot |
研究背景
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC.
Autoubiquitinated.
Cell membrane>Single-pass membrane protein. Cytoplasmic vesicle membrane.
Interacts with UBE2L3.
Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.
Belongs to the RBR family. RNF144 subfamily.
限制条款
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