HES1 Antibody | Affinity Biosciences LTD|亲科生物官网

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1/5

DF7569 at 1/100 staining Human gastric tissue by IHC-P.

FIGURE 5| The expression of VEGF‐Notch signaling pathway related proteins (VEGF, VEGFR, Notch‐1, Jagged1, Delta‐like1, and hes1)in HUVECs cocultured with VEGF/Notch shRNA transfected BMSCs (n = 3).

Fig.

FIGURE 7 | Hes1 expression is negatively regulated by miR-9 in NSCs.

Figure 6 Minocycline alleviates sevoflurane-induced neuroinflammation via Notch signaling suppression: (A) Cell immunofluorescence was performed to assess Notch signaling-associated proteins in BV2 cells of each group.

产品:	HES1 抗体
货号:	DF7569
描述:	Rabbit polyclonal antibody to HES1
应用:	WB IHC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量:	30 kDa; 30kD(Calculated).
蛋白号:	Q14469
RRID:	AB_2841062

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1500	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(92%)

克隆:

Polyclonal

特异性:

HES1 Antibody detects endogenous levels of total HES1.

RRID:

AB_2841062
引用格式: Affinity Biosciences Cat# DF7569, RRID:AB_2841062.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

bHLHb39; C-HAIRY1; c-hairy1A; Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1 (Drosophila); Hairy and enhancer of split 1; Hairy homolog (Drosophila); Hairy homolog; Hairy like; Hairy, Drosophila, homolog of; Hairy-like protein; Hairy/enhancer of split, Drosophila, homolog of, 1; HAIRY1; HES-1; hes1; Hes1 hairy and enhancer of split 1 (Drosophila); HES1_HUMAN; HHL; HL; HRY; MGC129109; OTTHUMP00000209031; RHL; Transcription factor HES-1;

抗原和靶标

免疫原:

Uniprot:

Q14469(HES1_HUMAN) >>Visit HPA database.

基因/基因ID:

HES1(3280)

序列:

Q14469 HES1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPADIMEKNSSSPVAATPASVNTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSRHSKLEKADILEMTVKHLRNLQRAQMTAALSTDPSVLGKYRAGFSECMNEVTRFLSTCEGVNTEVRTRLLGHLANCMTQINAMTYPGQPHPALQAPPPPPPGPGGPQHAPFAPPPPLVPIPGGAAPPPGGAPCKLGSQAGEAAKVFGGFQVVPAPDGQFAFLIPNGAFAHSGPVIPVYTSNSGTSVGPNAVSPSSGPSLTADSMWRPWRN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Chicken

100

Rabbit

100

Xenopus

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14469 作为底物

Q14469

Site	PTM Type	Enzyme	Source
S10	Phosphorylation		Uniprot
S11	Phosphorylation		Uniprot
S12	Phosphorylation		Uniprot
T24	Phosphorylation		Uniprot
S32	Phosphorylation		Uniprot
S37	Phosphorylation	P17252 (PRKCA)	Uniprot
S38	Phosphorylation	P17252 (PRKCA)	Uniprot
S55	Phosphorylation		Uniprot
T59	Phosphorylation		Uniprot
K77	Ubiquitination		Uniprot
K86	Ubiquitination		Uniprot
T97	Phosphorylation		Uniprot
K109	Ubiquitination		Uniprot

研究背景

Q14469_HES1_HUMAN

功能:

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

细胞定位:

Nucleus.

亚基结构:

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family. Interacts (via WPRW motif) with TLE1, and more weakly with TLE2. Interacts with HES6 (By similarity). Interacts with SIRT1. Interacts with an FA complex, composed of FANCA, FANCF, FANCG and FANCL, but not of FANCC, nor FANCE.

蛋白家族:

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).

The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.

The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.

研究领域

· Environmental Information Processing > Signal transduction > Notch signaling pathway. (View pathway)

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

文献引用

1). Notch1 contributes to TNF-α-induced proliferation and migration of airway smooth muscle cells through regulation of the Hes1/PTEN axis. International Immunopharmacology, 2020 (PubMed: 32871474) [IF=5.6]

Application: WB Species: mouse Sample: ASM cells

Fig. 5.| Notch1 knockdown decreased Hes1 and increased PTEN expression in TNF-α-induced ASM cells. ASM cells were transfected with NC or Notch1 siRNA for 48 h and then stimulated with TNF-α for 24 h. (D, E) Protein expression of Hes1 was examined by western blot.

2). Neural stem cell-derived exosomes regulate neural stem cell differentiation through miR-9-Hes1 axis. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34055767) [IF=5.5]

Application: WB Species: mouse Sample: NSCs

FIGURE 7 | Hes1 expression is negatively regulated by miR-9 in NSCs.(C) Representative western blotting results showing the expression of Hes1 and β-actin proteins in either agomiR-C- or agomiR-9-transfected NSCs.

3). TMT-based quantitative proteomics analysis reveals the role of Notch signaling in FAdV-4-infected LMH cell. Frontiers in Microbiology, 2022 (PubMed: 36187945) [IF=5.2]

4). The regulatory roles of VEGF-Notch signaling pathway on aplastic anemia with kidney deficiency and blood stasis. JOURNAL OF CELLULAR BIOCHEMISTRY, 2019 (PubMed: 30230583) [IF=4.0]

Application: WB Species: human Sample: HUVECs

FIGURE 5| The expression of VEGF‐Notch signaling pathway related proteins (VEGF, VEGFR, Notch‐1, Jagged1, Delta‐like1, and hes1)in HUVECs cocultured with VEGF/Notch shRNA transfected BMSCs (n = 3). *P < 0.05 vs HUVECs (normal control); #P < 0.05 vs BMSC + NC shRNA + HUVEC. BMSC, bone marrow mesenchymal stem cell; HUVEC, human umbilical vein endothelial cell; NC, normal control; shRNA, short hairpin RNA; VEGF, vascular endothelial growth factor; VEGFR, VEGF receptor

5). Minocycline Attenuates Sevoflurane-Induced Postoperative Cognitive Dysfunction in Aged Mice by Suppressing Hippocampal Apoptosis and the Notch Signaling Pathway-Mediated Neuroinflammation. Brain Sciences, 2023 (PubMed: 36979321) [IF=3.3]

Application: WB Species: Mouse Sample: hippocampal tissue

Figure 6 Minocycline alleviates sevoflurane-induced neuroinflammation via Notch signaling suppression: (A) Cell immunofluorescence was performed to assess Notch signaling-associated proteins in BV2 cells of each group. Scale bar, 20 μm; (B–G) Immunoblot detection of Notch1, cleaved Notch1, and Hes1 in BV2 cells and hippocampal tissue specimens. β-actin was utilized for normalizing data quantitated by densitometry with ImageJ. Data are mean ± SEM.

6). Astragalus Polysaccharide Promotes Neuronal Injury Repair via the Notch1/NFκB Signaling Axis in the Ventroposterior Thalamic Nucleus in Rats with Focal Cerebral Ischemia. Journal of integrative neuroscience, 2024 (PubMed: 38419443) [IF=1.8]

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HES1 Antibody - #DF7569

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