MCP1 Antibody | Affinity Biosciences LTD|亲科生物官网

产品:	MCP1 抗体
货号:	DF7577
描述:	Rabbit polyclonal antibody to MCP1
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Horse
分子量:	14 kDa; 11kD(Calculated).
蛋白号:	P13500
RRID:	AB_2841069

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二抗
封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

联系销售

实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Horse(83%)

克隆:

Polyclonal

特异性:

MCP1 Antibody detects endogenous levels of total MCP1.

RRID:

AB_2841069
引用格式: Affinity Biosciences Cat# DF7577, RRID:AB_2841069.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

C-C motif chemokine 2; CCL2; CCL2_HUMAN; Chemokine (C C motif) ligand 2; GDCF 2; GDCF-2; GDCF2; HC11; HSMCR30; JE; MCAF; MCP 1; MCP-1; MCP1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je); Small inducible cytokine A2; Small inducible cytokine subfamily A (Cys Cys), member 2; Small-inducible cytokine A2; SMC CF; SMC-CF; SMCCF;

抗原和靶标

免疫原:

A synthesized peptide derived from human MCP1, corresponding to a region within the internal amino acids.

Uniprot:

P13500(CCL2_HUMAN) >>Visit HPA database.

基因/基因ID:

CCL2(6347)

表达:

P13500 CCL2_HUMAN:

Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level) (PubMed:23765988). Expressed in monocytes (PubMed:2513477).

序列:

P13500 CCL2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKVSAALLCLLLIAATFIPQGLAQPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Horse

Pig

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

P13500_CCL2_HUMAN

功能:

Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

翻译修饰:

Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

N-Glycosylated.

细胞定位:

Secreted.

组织特异性:

Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level). Expressed in monocytes.

亚基结构:

Monomer or homodimer; in equilibrium. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.

蛋白家族:

Belongs to the intercrine beta (chemokine CC) family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

文献引用

1). Outer membrane vesicles derived from gut microbiota mediate tubulointerstitial inflammation: a potential new mechanism for diabetic kidney disease. Theranostics, 2023 (PubMed: 37554279) [IF=12.4]

Application: IHC Species: Rat Sample:

Figure 1 Gut microbiota depletion attenuates systemic and tubulointerstitial inflammation in diabetic rats. Gut microbiota of DM rats was depleted by treatment with broad-spectrum antibiotics administered in the drinking water (DM+AB). (A) Serum inflammatory cytokines, including IL-1β, TNF-α, MCP-1 and IL-6, were measured by ELISA (n=4). (B) Tubulointerstitial inflammation was detected by immunohistochemical staining (scale bar, 500 μm and 100 μm, original magnification × 200). (C-D) Expression levels of inflammatory proteins in renal cortex were detected by Western blot analysis and quantified (n=4-5). (E-F) Histopathological injuries of tubulointerstitium were evaluated by PAS staining (scale bar, 500 μm and 100 μm, original magnification × 200) and quantified (n=5).

Application: WB Species: Rat Sample:

2). Micheliolide Ameliorates Diabetic Kidney Disease by Inhibiting Mtdh-mediated Renal Inflammation in Type 2 Diabetic db/db Mice. PHARMACOLOGICAL RESEARCH, 2019 (PubMed: 31669149) [IF=9.3]

3). Inhibition of miR-155-5p Exerts Anti-Fibrotic Effects in Silicotic Rats by Regulating Meprin α. Molecular Therapy-Nucleic Acids, 2019 (PubMed: 31877411) [IF=8.8]

Application: WB Species: Rat Sample: RAW264.7 cells

Figure 4. miR-155-5p Negatively Regulates Mep1a (A) Protein expression of meprin a, pro-COL I, TGF-b1, a-SMA, and MCP-1 was detected by western blotting and quantified. (B) Levels of miR-155-5p and Mep1a in rat lungs. Data are presented as the mean ± SD. n = 5 per group. (C and D) Protein (C) and (D) mRNA (D) levels of meprin a in RAW264.7 cells treated with agomiR-155-5p or antamiR-155-5p. (E) Luciferase reporter assay demonstrating Mep1a was a target of miR-155-5p. Data are presented as the mean ± SD. n = 3 per group

4). Interfering microRNA-410 attenuates atherosclerosis via the HDAC1/KLF5/IKBα/NF-κB axis. Molecular Therapy - Nucleic Acids, 2021 (PubMed: 33981482) [IF=8.8]

Application: WB Species: Mouse Sample: aorta tissues

Figure 2 miR-40 inhibition suppresses the initiation of atherosclerosis in mice (A) Detection of miR-410 expression level in aorta tissues. (B) Arterial impairment and inflammation. (C) The area of aorta impairment. (D) Serum TNF-α, IL-1, and IL-6 levels in mice. (E) VCAM-1, ICAM-1, and MCP-1 expression in aorta tissues; ∗p < 0.05. Measured data are described as mean ± standard deviation, analyzed using independent samples t test between two groups; n = 10.

5). HIF-1α/JMJD1A signaling regulates inflammation and oxidative stress following hyperglycemia and hypoxia-induced vascular cell injury. CELLULAR & MOLECULAR BIOLOGY LETTERS, 2021 (PubMed: 34479471) [IF=8.3]

Application: WB Species: human Sample: HUVECs

Fig. 3 | Efects of inhibition of HIF-1α on expression of infammation after hyperglycemic and hypoxia.b Cells were treated with KC7F2 (10 µM) or si-HIF-1α for 48 h following combined stimulation, and IL-6, IL-8, ICAM-1, MCP-1, and c HIF-1α levels were analyzed. The relative density of IL-6, IL-8,ICAM-1, and MCP-1 (d) was normalized according to GAPDH expression.

Application: WB Species: Human Sample: HUVECs

Fig. 3 Effects of inhibition of HIF-1α on expression of inflammation after hyperglycemic and hypoxia. a Cells were treated with glucose (25 mM) or with combined exposure to high glucose and hypoxia for 6, 12, 24, and 48 h. Exposure to glucose alone or combined stimulation with hypoxia significantly increased gene expression of HIF-1α. b Cells were treated with KC7F2 (10 µM) or si-HIF-1α for 48 h following combined stimulation, and IL-6, IL-8, ICAM-1, MCP-1, and c HIF-1α levels were analyzed. The relative density of IL-6, IL-8, ICAM-1, and MCP-1 (d) was normalized according to GAPDH expression. Importantly, the downregulation of HIF-1α decreased the secretion of IL-6, IL-8, ICAM-1 and MCP-1 based on ELISA and qRT-PCR assays (e–f). n = 3; *p < 0.05 and **p < 0.01 vs. DMSO. DMSO-treated cells, DMSO; HIF-1α inhibitors KC7F2 (10 µM)-treated cells, KC7F2 (10 µM); si-HIF-1α, small interfering RNA HIF-1α; HG, high glucose; HG + Hypoxia, combined stimulus with high glucose and hypoxia; NG, control

6). Acetyl-CoA synthetase 2 promotes diabetic renal tubular injury in mice by rewiring fatty acid metabolism through SIRT1/ChREBP pathway. Acta Pharmacologica Sinica, 2023 (PubMed: 37770579) [IF=8.2]

7). A bioactive xyloglucan polysaccharide hydrogel mechanically enhanced by Pluronic F127 micelles for promoting chronic wound healing. International journal of biological macromolecules, 2024 (PubMed: 39047998) [IF=8.2]

8). Natural flavonol fisetin attenuated hyperuricemic nephropathy via inhibiting IL-6/JAK2/STAT3 and TGF-β/SMAD3 signaling. PHYTOMEDICINE, 2021 (PubMed: 33994251) [IF=7.9]

Application: WB Species: Mice Sample: kidneys

Fig. 3. Effects of fisetin on proinflammatory production in the kidneys of HN mice. (A) The mRNA expression levels of IL-6, TNF-α, and MCP-1 in renal tissue were analyzed by RT-PCR. (B) The protein expression levels of IL-6, TNF-α, and MCP-1were detected by western blot. (C-E) The ratio of IL-6, TNF-α, and MCP-1 to GAPDH was calculated. Data are expressed as mean ± SEM (n = 6). **** p < 0.0001 vs. control; #### p < 0.0001 vs. model. The ns. means no significance.

9). Kaempferol alleviates calcium oxalate crystal-induced renal injury and crystal deposition via regulation of the AR/NOX2 signaling pathway. PHYTOMEDICINE, 2021 (PubMed: 33852977) [IF=7.9]

Application: WB Species: Human Sample: HK-2 cells

Fig. 4. Kaempferol inhibited renal oxidative stress and inflammation via regulating NOX2 expression in vitro. (a-b) The intracellular ROS level was detected by flow cytometry after loading with DCFH-DA. Moreover, (c) H2O2 expression, as well as (d) GSH, SOD and MDA levels were examined. Afterwards, (e-l) the mRNA levels of IL-1β, IL-6, TNF-α, IL-10, IL-4, Arg1, OPN and CD44 were measured by RT-qPCR, and (m) The RT-qPCR of NOX subunits was analyzed after the exposure of COM crystals (400 μg/ml) or 0.5 mM oxalate with or without kaempferol (40 μM) for 12 h. (n, o) The NOX2, iNOS, p-NFκB-p65 and MCP-1 expressions in HK-2 cells were measured by WB. Data were expressed as the fold changes of the experimental group to the NC group (except part b) and were represented as means ± SD of three independent experiments with different cell passages on different days. ∆ p < 0.05; ∆∆ p < 0.01; * p < 0.05 vs. NC group; # p < 0.05 vs. COM group; ** p < 0.05 as pairwise comparisons of three groups among COM + Kae (10 μM), COM + Kae (20 μM), and COM + Kae (40 μM).

10). Pyrogallol enhances therapeutic effect of human umbilical cord mesenchymal stem cells against LPS-mediated inflammation and lung injury via activation of Nrf2/HO-1 signaling. Free radical biology & medicine, 2022 (PubMed: 36028178) [IF=7.4]

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MCP1 Antibody - #DF7577

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