| 产品: | EPN1 抗体 |
| 货号: | AF0700 |
| 描述: | Rabbit polyclonal antibody to EPN1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Dog |
| 分子量: | 85kDa; 60kD(Calculated). |
| 蛋白号: | Q9Y6I3 |
| RRID: | AB_2834389 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0700, RRID:AB_2834389.
展开/折叠
EH domain binding mitotic phosphoprotein; EH domain-binding mitotic phosphoprotein; EPN 1; Epn1; EPN1_HUMAN; EPS 15 interacting protein 1; EPS-15-interacting protein 1; Epsin 1; Epsin-1;
抗原和靶标
- Q9Y6I3 EPN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTSSLRRQMKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIADLTYNVVAFSEIMSMIWKRLNDHGKNWRHVYKAMTLMEYLIKTGSERVSQQCKENMYAVQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDRLREERAHALKTKEKLAQTATASSAAVGSGPPPEAEQAWPQSSGEEELQLQLALAMSKEEADQPPSCGPEDDAQLQLALSLSREEHDKEERIRRGDDLRLQMAIEESKRETGGKEESSLMDLADVFTAPAPAPTTDPWGGPAPMAAAVPTAAPTSDPWGGPPVPPAADPWGGPAPTPASGDPWRPAAPAGPSVDPWGGTPAPAAGEGPTPDPWGSSDGGVPVSGPSASDPWTPAPAFSDPWGGSPAKPSTNGTTAAGGFDTEPDEFSDFDRLRTALPTSGSSAGELELLAGEVPARSPGAFDMSGVRGSLAEAVGSPPPAATPTPTPPTRKTPESFLGPNAALVDLDSLVSRPGPTPPGAKASNPFLPGGGPATGPSVTNPFQPAPPATLTLNQLRLSPVPPVPGAPPTYISPLGGGPGLPPMMPPGPPAPNTNPFLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y6I3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K11 | Ubiquitination | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| Y101 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| S382 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T412 | Phosphorylation | Uniprot | |
| T416 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| T460 | Phosphorylation | Uniprot | |
| T462 | Phosphorylation | Uniprot | |
| T464 | Phosphorylation | Uniprot | |
| T467 | Phosphorylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| T470 | Phosphorylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| T494 | Phosphorylation | Uniprot | |
| S501 | Phosphorylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| Y548 | Phosphorylation | Uniprot |
研究背景
Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.
Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
Ubiquitinated.
Cytoplasm. Cell membrane>Peripheral membrane protein. Nucleus. Membrane>Clathrin-coated pit.
Note: Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity).
Monomer. Binds clathrin, ZBTB16/ZNF145 and ITSN1 (By similarity). Binds ubiquitinated proteins (By similarity). Binds REPS2, EPS15, AP2A1 and AP2A2. Interacts with RALBP1 in a complex also containing NUMB and TFAP2A during interphase and mitosis. Interacts with AP2B1. Interacts with UBQLN2.
The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP2A2 and clathrin binding.
The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction with the AP-2 complex subunit AP2B1.
Belongs to the epsin family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.